Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures

Belhadj, Assia; Télef, Nadège; Saigne, Cassandrine; Cluzet, Stéphanie; Barrieu, François; Hamdi, Saı̈d; Mérillon, Jean‐Michel

doi:10.1016/j.plaphy.2007.12.001

Cited by 195 publications

(171 citation statements)

References 35 publications

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“…A vast range of abiotic stress treatments lead to stilbene biosynthesis, including mechanical damage (Chiron et al, 2000;Pezet et al, 2003), exposure to UV-C light (Bais et al, 2000), treatment with chemicals, such as aluminum ions, cyclodextrins, and ozone (Rosemann et al, 1991;Adrian et al, 1996;Zamboni et al, 2009), and the application of plant hormones like ethylene and jasmonates (Belhadj et al, 2008a(Belhadj et al, , 2008bD'Onofrio et al, 2009). In terms of biotic stresses, the synthesis of stilbenes is particularly well documented in grapevine, where it has been shown to be induced upon infection with various pathogens, including Plasmopara viticola (Langcake and Pryce, 1976), Erisyphe necator (Fung et al, 2008;Schnee et al, 2008), and Botrytis cinerea .…”

Section: Introductionmentioning

confidence: 99%

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

et al. 2013

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Plant stilbenes are phytoalexins that accumulate in a small number of plant species, including grapevine (Vitis vinifera), in response to biotic and abiotic stresses and have been implicated in many beneficial effects on human health. In particular, resveratrol, the basic unit of all other complex stilbenes, has received widespread attention because of its cardio-protective, anticarcinogenic, and antioxidant properties. Although stilbene synthases (STSs), the key enzymes responsible for resveratrol biosynthesis, have been isolated and characterized from several plant species, the transcriptional regulation underlying stilbene biosynthesis is unknown. Here, we report the identification and functional characterization of two R2R3-MYB-type transcription factors (TFs) from grapevine, which regulate the stilbene biosynthetic pathway. These TFs, designated MYB14 and MYB15, strongly coexpress with STS genes, both in leaf tissues under biotic and abiotic stress and in the skin and seed of healthy developing berries during maturation. In transient gene reporter assays, MYB14 and MYB15 were demonstrated to specifically activate the promoters of STS genes, and the ectopic expression of MYB15 in grapevine hairy roots resulted in increased STS expression and in the accumulation of glycosylated stilbenes in planta. These results demonstrate the involvement of MYB14 and MYB15 in the transcriptional regulation of stilbene biosynthesis in grapevine.

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Section: Introductionmentioning

confidence: 99%

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

et al. 2013

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“…Loivamäki et al (2004) also reported that jasmonate induction led to an increase in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuate. Moreover, the impacts of MeJA on the biosynthesis of volatile compounds in climacteric and non-climacteric fruits, such as apples and strawberries, respectively, have also been reported (Ayala-Zavala et al, 2005;Kim et al, 2006;de la Peña Moreno et al, 2010a;2010b;Belhadj et al, 2008;Gohain et al, 2012). However, investigations toward the effects of exogenous MeJA on tea aroma have been scarcely reported.…”

Section: Introductionmentioning

confidence: 99%

Aroma changes of black tea prepared from methyl jasmonate treated tea plants

Shi

Wang²,

et al. 2014

J. Zhejiang Univ. Sci. B

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Methyl jasmonate (MeJA) was widely applied in promoting food quality. Aroma is one of the key indicators in judging the quality of tea. This study examined the effect of exogenous MeJA treatment on tea aroma. The aroma components in black tea prepared from MeJA-treated fresh tea leaves were extracted using headspace solid-phase microextraction (HS-SPME) and were analyzed using gas chromatography-mass spectrometry (GC-MS) and GC-olfactometry (GC-O). Forty-five volatile compounds were identified. The results revealed that the MeJA-treated black tea had higher levels of terpene alcohols and hexenyl esters than the untreated tea. Moreover, several newly components, including copaene, cubenol, and indole, were induced by the MeJA treatment. The activities of polyphenol oxidase and β-glucosidase in fresh tea leaves changed after the MeJA treatment. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the gene expression levels of polyphenol oxidase and β-primeverosidase were upregulated by two and three folds, respectively, by the MeJA treatment (P<0.01); however, the gene expression of β-glucosidase was downregulated to a half level. In general, the aroma quality of the MeJAtreated black tea was clearly improved.

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“…In this case, the endogenous trans-resveratrol accumulation was around 24 µg/g dry weight (DW) and the transresveratrol secreted to the medium was over 8 µg/g DW. In a similar way, Belhadj et al, (2008) described the production of 0.6 mg trans-resveratrol/g DW in V. vinifera cv Gamay elicited with MJ in a culture medium with the sugar concentration increased. However, the most significant success in increasing trans-resveratrol content in grapevine cell cultures has been reached using CDs alone or in combination with MJ .…”

Section: Wwwintechopencommentioning

confidence: 68%

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

Almagro¹,

Sabater-Jara²,

Belchí-Navarro³

et al. 2011

Plants and Environment

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Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures

Cited by 195 publications

References 35 publications

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

Aroma changes of black tea prepared from methyl jasmonate treated tea plants

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

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