Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

Almagro, Lorena; Sabater-Jara, Ana Belén; Belchí-Navarro, Sarai; Fernández-Pérez, Francisco; Bru, Roque; Pedreño, María A.

doi:10.5772/24231

Cited by 12 publications

(14 citation statements)

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“…Both abiotic and biotic elicitors have been extensively used for improving TIA production in C. roseus in vitro cultures since the beginning of the 1990s [ 105 , 107 , 108 , 109 ]. In fact, abiotic elicitors like metal ions and UV light, as well as biotic elicitors such as fungal preparations and yeast extracts, hydrolytic enzymes, chemicals (cyclodextrins (CDs) and signalling molecules like nitric oxide, jasmonic acid and derived compounds such as methyl jasmonate (MeJA) and acetylsalicylic acid were commonly used [ 73 , 84 , 85 , 110 , 111 , 112 ]. In this way, the treatment of C. roseus hairy root cultures with 0.1 mM sodium nitroprusside (NO donor) caused a significant increase of serpentine, catharanthine, ajmalicine, hörhammericine, lochnericine and tabersonine [ 78 ] ( Table 1 ).…”

Section: Empirical Strategies For Improving Tia Productionmentioning

confidence: 99%

“…In the same way, Lee-Parsons et al [ 110 ] showed that the addition of 100 µM MeJA to C. roseus cell cultures increased the ajmalicine extracellular production (10.2 mg/L) in a dose and time-dependent manner. MeJA also triggered the production of ajmalicine (137.2 mg/L) and catharanthine (55.6 mg/L) in C. roseus cell cultures [ 73 ]. This elicitor was also effective in accumulating ajmalicine (6.34 mg/g DW) serpentine (1.71 mg/g DW), ajmaline (12 mg/g DW) and catharanthine (4.34 mg/g DW) in C. roseus hairy roots elicited with 250 μM MeJA [ 81 ] ( Table 1 ).…”

Section: Empirical Strategies For Improving Tia Productionmentioning

confidence: 99%

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Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

2015

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Catharanthus roseus is a medicinal plant belonging to the family Apocynaceae which produces terpenoid indole alkaloids (TIAs) of high medicinal importance. Indeed, a number of activities like antidiabetic, bactericide and antihypertensive are linked to C. roseus. Nevertheless, the high added value of this plant is based on its enormous pharmaceutical interest, producing more than 130 TIAs, some of which exhibit strong pharmacological activities. The most striking biological activity investigated has been the antitumour effect of dimeric alkaloids such as anhydrovinblastine, vinblastine and vincristine which are already in pre-, clinical or in use. The great pharmacological importance of these indole alkaloids, contrasts with the small amounts of them found in this plant, making their extraction a very expensive process. To overcome this problem, researches have looked for alternative sources and strategies to produce them in higher amounts. In this sense, intensive research on the biosynthesis of TIAs and the regulation of their pathways has been developed with the aim to increase by biotechnological approaches, the production of these high added value compounds. This review is focused on the different strategies which improve TIA production, and in the analysis of the beneficial effects that these compounds exert on human health.

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Section: Empirical Strategies For Improving Tia Productionmentioning

confidence: 99%

Section: Empirical Strategies For Improving Tia Productionmentioning

confidence: 99%

Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

2015

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“…In addition, the high levels of trans-R whih is secreted and accumulated in the culture medium have no toxic effect on the cell lines, allowing successful subcultures. In fact, CD act not only as inducers of trans-R biosynthesis but also as promoters of adducts that remove trans-R from the medium, reducing feedback inhibition and trans-R degradation and allowing its accumulation in high concentrations (Almagro et al, 2011). Interestingly, Lijavetzky et al (2008) demonstrated that the combined use of CD and methyl jasmonate enhanced the production of trans-R, which was strongly associated to an increased expression of STS, PAL, C4H, 4CL genes in grapevine SCC.…”

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confidence: 99%

Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine

Almagro

Belchí-Navarro

Martínez-Márquez

et al. 2015

Plant Physiology and Biochemistry

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In the present work the effect of cyclodextrin and coronatine on both trans-resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans-resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans-resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans-resveratrol in the spent medium was not detected. Therefore, despite the fact that trans-resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans-R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.

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“…Light of short wavelengths had been reported to be environment stress and accelerate accumulation of many secondary metabolic compounds (Almagro et al 2011). So blue light or light of short wavelengths should be used in the cultivation of medicinal fungi rather than only traditional daylight.…”

Section: Discussionmentioning

confidence: 99%

“…Daylight is traditionally used to stimulate fruit body development in the cultivation of C. militaris. However, in plant research, light of different wavelengths, especially red light and blue light, had been reported to regulate some metabolic pathways to enhance some active components accumulation (Almagro et al 2011;Lin 2002). There had been several articles on blue light regulating physiological process, body formation and gene expression of fungi (Idnurm et al 2006;Cheng et al 2002;Velayos et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Light Wavelengths Regulate Growth and Active Components of Cordyceps militaris Fruit Bodies

Dong

Lei

Zheng

et al. 2012

Journal of Food Biochemistry

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Cordyceps militaris is widely cultivated for functional food in China and Southeast Asia. Daylight was commonly used for development of C. militaris fruit bodies. In this study, effects of light wavelengths (pink light = 1/3,450–460 nm + 2/3,620–630 nm; red light = 620–630 nm; blue light = 450–460 nm) on fruit body's production and contents of bioactive components were investigated. The results are: pink light increased dried matter contents and bioefficiency of fruit bodies to the highest (40.06/6.77%) compared with blue light (24.44/4.86%), daylight (22.69/4.55%) and red light (22.06/4.17%), respectively. Pink light significantly enhanced accumulation of carotenoids. Red light obtained the highest contents of adenosine. Pink light is the most optimal for cordycepin accumulation. In order to get the highest production of adenosine, cordycepin, carotenoids and fruit body at the same time, pink light rather than only traditional daylight should be used in the fruit body development, or light wavelengths were regulated according to target compounds of the fruit bodies. Practical Applications This study indicated that different light wavelengths have significant influences on fruit body development and accumulation of the main active compounds as cordycepin, adenosine and carotenoids. Pink light (1/3,450–460 nm + 2/3,620–630 nm) was optimal for production of adenosine, cordycepin and carotenoids. While the traditionally used daylight proved not suitable for cultivation of Cordyceps militaris. These results provided technological basis for cultivation of C. militaris and a insight to light reaction study on light wavelengths regulating accumulation of secondary metabolites.

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Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

Cited by 12 publications

References 88 publications

Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine

Light Wavelengths Regulate Growth and Active Components of Cordyceps militaris Fruit Bodies

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