Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes in Mentha spicata hairy root cultures

Yousefian, Shirin; Lohrasebi, Tahmineh; Farhadpour, Mohsen; Haghbeen, Kamahldin

doi:10.1007/s11240-020-01856-9

Cited by 47 publications

(23 citation statements)

References 47 publications

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“…Biotechnological platforms based on plant cell cultures have been successfully developed for several medicinal plant species [ 30 , 82 , 127 , 128 ]. Among the methods used to improve the biotechnological production of SMs are screening and selection of highly productive cell lines, as well as the optimization of culture conditions and induction of secondary metabolism by the use of elicitors [ 54 , 55 , 128 ]. When undifferentiated cultures such as calli and cell suspensions are used to produce the target compounds, results are often poor.…”

Section: Approaches To the Biotechnological Production Of Rosmarinmentioning

confidence: 99%

“…These in vitro techniques have been widely used for the production of several SMs, notably taxol [ 31 , 32 , 33 ], podophyllotoxin [ 34 , 35 , 36 ], withanolides [ 37 ], centellosides [ 38 , 39 ], and rosmarinic acid (RA) [ 40 , 41 , 42 , 43 ], as well as for mass propagation [ 44 ], in vitro cloning [ 45 , 46 , 47 ], and polyploidization [ 48 , 49 ]. Many RA-producing biotechnological platforms have been established, based on shoots [ 50 , 51 , 52 ], callus [ 53 ], cells [ 41 , 42 , 54 ], and hairy root cultures [ 55 , 56 , 57 , 58 ] of numerous species of the Boraginaceae, Anthocerotaceae, and Lamiaceae.…”

Section: Introductionmentioning

confidence: 99%

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Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

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Modern lifestyle factors, such as physical inactivity, obesity, smoking, and exposure to environmental pollution, induce excessive generation of free radicals and reactive oxygen species (ROS) in the body. These by-products of oxygen metabolism play a key role in the development of various human diseases such as cancer, diabetes, heart failure, brain damage, muscle problems, premature aging, eye injuries, and a weakened immune system. Synthetic and natural antioxidants, which act as free radical scavengers, are widely used in the food and beverage industries. The toxicity and carcinogenic effects of some synthetic antioxidants have generated interest in natural alternatives, especially plant-derived polyphenols (e.g., phenolic acids, flavonoids, stilbenes, tannins, coumarins, lignins, lignans, quinines, curcuminoids, chalcones, and essential oil terpenoids). This review focuses on the well-known phenolic antioxidant rosmarinic acid (RA), an ester of caffeic acid and (R)-(+)-3-(3,4-dihydroxyphenyl) lactic acid, describing its wide distribution in thirty-nine plant families and the potential productivity of plant sources. A botanical and phytochemical description is provided of a new rich source of RA, Satureja khuzistanica Jamzad (Lamiaceae). Recently reported approaches to the biotechnological production of RA are summarized, highlighting the establishment of cell suspension cultures of S. khuzistanica as an RA chemical biofactory.

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Section: Approaches To the Biotechnological Production Of Rosmarinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

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“…JA and MeJA are plant signaling compounds that affect plant growth and gene expression [28]. Several studies have reported that MeJA is a potent elicitor of resveratrol production [29][30][31]. Ho et al [32] reported that elicitation by MeJA begins in the plasma membrane, where the specific receptor receives the signal and switches on the defense mechanism in the plant; however, the signal transduction process elicited by MeJA is currently unclear.…”

Section: Role Of Meja In Rs Gene Expression Relative To Total Resveratrol Productionmentioning

confidence: 99%

Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension

Kantayos

Kim

Baek

2021

Plants

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Resveratrol, a secondary plant metabolite, and its derivatives, including piceid, show several potential health-related biological activities. However, resveratrol production is uncommon in plants; thus, resveratrol-enriched rice (DJ526) is produced for its nutritional and therapeutic value. Here, a DJ526 cell suspension was treated with various elicitors to determine its resveratrol-production potential and elicit its biological activity. Treatments with most elicitors produced more piceid than resveratrol; as elicitation periods increased, the average piceid levels were 75-fold higher than resveratrol levels. This increase is associated with glycosylation during growth and development. The duration of exposure and concentrations of elicitors were crucial factors affecting resveratrol synthase expression. Of all the elicitors tested, jasmonic acid and methyl jasmonate (MeJA) were strong elicitors; they increased resveratrol production to ≤115.1 μg g−1 (total resveratrol and piceid content). Moreover, 5 μM of MeJA increased total resveratrol production by >96.4% relative to the control production. In addition, the extract of cell suspension treated with 5 μM of MeJA significantly reduced melanin content and cellular tyrosinase activity (24.2% and 21.5% relative to the control, respectively) in melan-a cells without disturbing cell viability. Overall, elicitation can enhance resveratrol production and elicit the biological activity of the compound, in this case, its anti-melanogenic activities, in DJ526 cell suspension.

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“…The well known elicitor methyl jasmonate (MeJA) is a phytohormone that contributes to the hyperproduction of secondary plant metabolites, as it plays an essential role in the regulation of specific genes, involved in plant development and defense mechanisms against different biotic and abiotic stresses (Ji et al, 2019;Baek et al, 2020). Although previous studies have examined the effects of MeJA on the production of UA, OA and RA in Lamiaceae family (Ruffoni et al, 2016;Li et al, 2020;Yousefian et al, 2020), few studies, with the exception of Ocimum basilicum suspension cultures (Pandey, Singh & Banerjee, 2019), have been designed to explore the feasibility of up-scaling the production of these three bioactive metabolites, using an in vitro culture system. Here, we describe the first protocol for the establishment of hairy root culture of L. caulescens and present a comparative analysis of the effects of different concentrations and times of exposure to MeJA on the production of UA, OA, and RA.…”

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

Vergara-Martínez

Estrada‐Soto

Valencia-Díaz

et al. 2021

PeerJ

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Background Ursolic (UA), oleanolic (OA) and rosmarinic (RA) acids are bioactive metabolites found in Lepechinia caulescens that have generated interest for their health benefits, which include antimicrobial, antioxidant, antimutagenic, gastroprotective, antidiabetic, antihypertensive and anti-inflammatory properties, among others. To date, very few attempts have been made to evaluate the potential for simultaneous production of these bioactive compounds, using a biotechnological approach. Hairy root cultures offer a biotechnology approach that can be used to study the factors affecting the biosynthesis and the production of UA, OA and RA. In the current study, we established hairy root cultures of L. caulescens and evaluated the effect of sucrose on biomass accumulation, and the effect of different concentrations and times of exposure of methyl jasmonate (MeJA), on the accumulation of UA, OA and RA. Methods Leaves from plants of L. caulescens were inoculated with Agrobacterium rhizogenes strain ATCC 15834. PCR of rolB gene confirmed the transgenic nature of hairy roots. Hairy roots were subcultured in semisolid MSB5 medium, supplemented with 15, 30, 45 or 60 g/L sucrose and after 4 weeks, dry weight was determined. The accumulation of UA, OA and RA of wild plants and hairy roots were determined by HPLC. Finally, the hairy roots were treated with 0, 100, 200 and 300 µM of MeJA and the content of bioactive compounds was analyzed, after 24, 48 and 72 h. Results High frequency transformation (75%) was achieved, using leaf explants from axenic seedlings, infected with A. rhizogenes. The hairy roots showed an enhanced linear biomass accumulation, in response to the increase in sucrose concentration. The hairy root cultures in MSB5 medium, supplemented with 45 g/L sucrose, were capable to synthesizing UA (0.29 ± 0.00 mg/g DW), OA (0.57 ± 0.00 mg/g DW) and RA (41.66 ± 0.31 mg/g DW), about two, seven and three times more, respectively, than in roots from wild plants. Elicitation time and concentration of MeJA resulted in significant enhancement in the production of UA, OA and RA, with treatments elicited for 24 h, with a concentration of 300 µM of MeJA, exhibiting greatest accumulation. Conclusion This is the first report on development of hairy root cultures of L. caulescens. Future studies should aim towards further improving triterpenes and polyphenolic compound production in hairy roots of L. caulescens, for use in the pharmaceutical and biotechnological industry.

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Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes in Mentha spicata hairy root cultures

Cited by 47 publications

References 47 publications

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

Enhancing Resveratrol Bioproduction and Anti-Melanogenic Activities through Elicitation in DJ526 Cell Suspension

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

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