Effect of micropore/microsphere topography and a silicon-incorporating modified titanium plate surface on the adhesion and osteogenic differentiation of BMSCs

Abstract

Good biological properties for titanium implants will shorten the treatment cycle and improve patient comfort, which are also the main goals of dentistry and orthopaedics. At present, the biological properties of titanium implants are mainly enhanced in two aspects: their surface chemistry and surface morphology. In this study, a surface modification strategy combining bioactive trace elements with surface micromorphology modification was used to enhance the biological properties of pure titanium. A new coating incorporating silicon micropore/microsphere topography was prepared on a titanium plate by micro-arc oxidation (MAO) technology. The properties of the coating and its effects on the adhesion and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were further analyzed. The experimental results show that a coating doped with amorphous silicon with micropore/ microsphere topography was incorporated onto the titanium surface and the surface roughness in the treated groups was obviously higher than that in the Ti group. In vitro, the presence of a silicon-incorporating coating with a micropore/microsphere topography on the titanium surface significantly enhanced the initial adhesion, proliferation and osteogenic differentiation of BMSCs. These results indicate that the silicon-incorporating coating with micropore/microsphere topography has potential applications in dentistry and orthopaedics.

Inhibition of Inflammatory Response and Promotion of Osteogenic Activity of Zinc-Doped Micro-Arc Titanium Oxide Coatings

“…Meanwhile, studies have shown that microporous coatings significantly enhance the initial bonding, proliferation, and osteogenic differentiation of BMSCs. 39 In most cases, a change in one aspect of the surface of a material is always accompanied by a significant difference in the properties of the other, making it difficult to determine the main factors that influence the response of the cell. 40 Therefore, the better physical properties of the MAO group, Zn1 group, and Zn2 group are also the result of various influences.…”

“…ALP staining was used to observe the effect of different treatment groups of titanium sheets on the early osteogenic effect of BMSCs. ALP was used as an indicator of early osteogenesis, 39 and the staining results showed that the Zn2 group had the darkest ALP staining, followed by the Zn1 and MAO groups, and finally by the pure titanium group ( Figure 6 a). The protein quantification results showed that the ALP protein expression was higher in the Zn1 and Zn2 groups than in the Ti group, but the Zn2 group also had a statistically significant increase relative to the MAO group ( Figure 6 b).…”

Biofunctional Elements Incorporated Nano/Microstructured Coatings on Titanium Implants with Enhanced Osteogenic and Antibacterial Performance

“…Si is mainly distributed in mitochondria and other intracellular organelles, which acts as an initiator for perosseous tissue mineralization by crosslinking collagen and proteoglycans and maintaining the stability of collagen in the early stage of bone formation. [151][152][153] Addition of Si into the coatings enhances the osteogenic differentiation of osteoblast and BMSCs [11,34,[154][155][156] and the angiogenic activity of HUVECs. [24,157] Moreover, the inflammatory response is demonstrated to reduce on the SiO 2 coatings.…”

Strontium‐Doped Hydroxyapatite Promotes Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporotic Rats through the CaSR‐JAK2/STAT3 Signaling Pathway

“…[ 10 ] A previous study reported that microcarriers could provide high surface areas for cell attachment, growth, and cell differentiation phenotype maintenance. [ 11 ] The study also showed that microcarrier topography was beneficial to the osteogenic differentiation of BMSCs. Besides, microcarriers are suitable for the delivery of bioactive molecules.…”