Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

Souza, Beatriz Dulcinéia Mendes de; Alves, Ana Maria Hecke; Ribeiro, Dayane Machado; Santos, Luciane Geanini Pena dos; Simões, Claudia Maria de Oliveira; Felippe, Wilson Tadeu; Felippe, Mara Cristina Santos

doi:10.1590/0103-6440201601164

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…For the cells stored in milk, their viability levels at 30 minutes and 1 hour were also similar, but there was decline in the cell viability between 1 and 3 hours (p<0.05). The latter observation may be due to a decline in the pH of milk over time 31 . However, the cells stored in egg white had a sequential decline in their viability, similar to the findings of Badakhsh et al 20 (2014), and this accounted for a sequential decline in the nutrients in egg white over time.…”

Section: Discussionmentioning

confidence: 99%

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Williams,

Denloye,

Popoola

et al. 2023

Braz. J. Oral Sci.

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To compare the viability of periodontal ligament (PDL) cells stored in Hanks Balanced Salt Solution (HBSS) with those in readily available transport media over a variable period of time. Methods: Periodontal ligament cells harvested from premolars freshly extracted for orthodontic reasons were cultured for exponential growth. The cells were exposed to egg white, evaporated milk, water and Hanks Balanced Salt Solution (HBSS) at room temperature. Their viability was evaluated after 30 minutes, 1 hour and 3 hours with the tetrazolium salt-based colorimetric assay (MTT assay). Statistical analysis was done using the IBM® SPSS version 23.0 software. Comparison between the Mean Optical Densities (MODs) of the cells stored in HBSS and other media at each time interval was done using the independent t test. Repeated measure ANOVA and Tukey post-hoc test were also carried out to compare the MOD of cells within each medium over time. The level of significance was set at p <0.05. Result: The PDL cells stored in egg white had higher MODs than those in HBSS at 30 minutes and 1 hour. Conversely, the MODs of the cells stored in milk and water were lower than those in HBSS at all the studied points. There was a significant difference in the viability of the cells stored in HBSS and water at all the time points (p<0.05). Conclusion: For up to an hour, egg white was found to perform better than HBSS in supporting the viability of PDL cells.

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Section: Discussionmentioning

confidence: 99%

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Williams,

Denloye,

Popoola

et al. 2023

Braz. J. Oral Sci.

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“…Este produto, possui propriedades fisiológicas, incluindo o pH e a osmolaridade, compatíveis com as células do ligamento periodontal. Além disso, é de fácil disponibilidade, e são livres de bactérias 16,17 . Embora 75% dos professores e 86,11% dos alunos soubessem que tempo e meio de armazenagem são fatores cruciais em casos de avulsão, os resultados mostraram que poucos professores tinham conhecimento sobre o tempo crítico para o reimplante e meio de armazenagem ideal.…”

Section: Discussionunclassified

Avaliação do conhecimento de professores e estudantes de graduação no manejo da avulsão

Andrade

Reis

2021

Rev Odontol Bras Central

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Objetivo: Avaliar o conhecimento e nível de experiência quanto ao manejo de dentes avulsionados dos docentes e acadêmicos de Odontologia da Universidade de Rio Verde-Goiás. Material e método: Um questionário impresso foi aplicado presencialmente pelos autores para 20 professores e 180 alunos, composto por informações sociodemográficas e questões específicas relacionadas à avulsão. Os dados foram tabulados e submetidos à análise estatística pelo teste Qui-quadrado. Resultados: Mais de 60% dos professores e alunos receberam treinamento em primeiros socorros para tratamento de traumatismos dentários, e sabem como proceder nesses casos. Quanto ao meio de armazenamento para dentes avulsionados, 35% dos professores e 44,44% dos estudantes responderam leite. Considerando que houve diferença significativa no grupo dos acadêmicos (p=0.007). Apenas 26,31% dos docentes e 21,11% dos acadêmicos conheciam sobre o tempo extra-alveolar para reimplante. Sobre o uso de contenção semirrígida em casos de avulsão, 75% dos professores e 61,66% dos estudantes responderam corretamente. O período de manutenção por 15 dias dessa contenção foi respondido por 57,89% dos professores e 48,33% dos estudantes. Neste ponto houve diferença estatisticamente no grupo dos alunos (p=0.006). Em relação ao tempo para realização do tratamento endodôntico, apenas 36,84% dos docentes e 31,66% dos estudantes, possuíam esse conhecimento. Considerando que houve diferença significativa no grupo dos acadêmicos (p=0.005). Sobre o tempo de acompanhamento radiográfico, 50% dos professores e 32,77% dos estudantes, responderam corretamente. Conclusão: O nível de conhecimento e experiência dos professores e estudantes foi insuficiente quanto à avulsão, necessitando-se de capacitação para o sucesso do prognóstico desses dentes.

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“…Then DMSO (Dimethylsulfoxide) (100 mL per well) was used to dissolve the precipitated formazan. Finally, the absorbance was measured by a micro-plate reader (Hercules, CA, USA) at 450 nm and the cell viability was determined using the following equation [51]:…”

Section: Cytocompatibilitymentioning

confidence: 99%

Layer-by-Layer Self-Assembly Composite Coatings for Improved Corrosion and Wear Resistance of Mg Alloy for Biomedical Applications

Tong-fang¹,

Sheng²,

Sheng³

2021

Coatings

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Mg alloys are promising biomedical metal due to their natural degradability, good processability, and favorable mechanical properties. However, the poor corrosion resistance limits their further clinical applications. In this study, the combined strategies of surface chemical treatment and layer-by-layer self-assembly were used to prepare composite coatings on Mg alloys to improve the biocorrosion resistance. Specially, alkalized AZ91 Mg alloy generated chemical linkage with silane via Si–O–Mg covalent bond at the interface. Subsequently, Si–OH group from silane formed a crosslinked silane layer by Si–O–Si network. Further chemical assembly with graphene oxide (GO), lengthened the diffusion pathway of corrosive medium. The chemically assembled composite coatings could firmly bond to Mg alloy substrate, which persistently and effectively acted as compact barriers against corrosion propagation. Improved biocorrosion resistance of AZ91 Mg alloy with self-assembly composite coatings of silane/GO was subsequently confirmed by immersion tests. Besides, the Mg alloy exhibited good wear resistance due to outside layer of GO with a lubricant effect. Cell viability of higher than 75% had also been found for the alloy with self-assembly composite coatings, which showed good cytocompatibility.

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Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

Cited by 5 publications

References 23 publications

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Avaliação do conhecimento de professores e estudantes de graduação no manejo da avulsão

Layer-by-Layer Self-Assembly Composite Coatings for Improved Corrosion and Wear Resistance of Mg Alloy for Biomedical Applications

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