Effect of miR-155 as a molecular adjuvant of DNA vaccine against VHSV in olive flounder (Paralichthys olivaceus)

Lim, Hyun Ju; Abdellaoui, Najib; Kim, Ki Hong

doi:10.1016/j.fsi.2019.02.056

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…The miRNAs miR‐155 has been used as a molecular adjuvant of DNA vaccine against VHSV infection in olive flounder (Lim et al . 2019). This shows the prominent role of miRNA in virus control, but the underlying mechanism is still unknown.…”

Section: Other Aspectsmentioning

confidence: 99%

“…When co‐immunizing fish with the DNA vaccine encoding VHSV G protein and miR‐155 as a molecular adjuvant, the level of antibody in the fish serum was greatly increased and the antiviral activity was more potent (Lim et al . 2019). This also illustrates the ability of microRNA in the antiviral field in fish aquaculture.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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MicroRNAs (miRNAs) are single-stranded non-coding RNA molecules. In addition to participating in various biological processes of eukaryotes, such as cell proliferation, development, differentiation, metabolism, tumorigenesis, and apoptosis, miRNAs also play an important role in the regulation of virus replication. Here, we review the current knowledge on high-throughput miRNA sequencing of teleost fish or fish cells in response to virus infection, the role of teleost fish miRNAs in regulating virus replication via targeting either fish or virus genes and the function of teleost fish virus-encoded miRNAs during virus infection. Understanding the interaction between the miRNAs and the host or virus genes will lead to the identification of novel strategy to prevent virus infection in teleost fish.

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Section: Other Aspectsmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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“…Additionally, miRNAs as a molecular adjuvant of vaccine have been recognized as a useful way to protect cultured fish from pathogen infection. In olive flounder, Lim et al (2019) examined the effect of miR‐155 on the antiviral activity and on the vaccine efficacy and found the miRNA acts as a molecular adjuvant of DNA vaccine against VHSV. The research will provide a new direction for the application of miRNAs in fish aquaculture farms.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

MicroRNA regulation of Toll‐like receptor, RIG‐I‐like receptor and Nod‐like receptor pathways in teleost fish

Chu

2020

Reviews in Aquaculture

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The innate immune system is the first‐line defence against invading pathogens, and the main molecules involved in recognizing invading pathogens are the pattern recognition receptors. Accumulating evidences have demonstrated that activation and termination of the immune responses are regulated by various layers of regulators and mechanisms. MicroRNAs (miRNAs) are a group of small non‐coding RNAs that emerged as critical component of the complex regulatory networks in fish immune responses. In this review, we have summarized miRNA regulation in teleost fish, which can provide new insights into the regulation of innate immune receptor pathways in fish. Meanwhile, we comprehensively discuss the recent researches on how teleost miRNAs respond to the signalling pathways of innate immune receptors, including Toll‐like receptors, retinoic acid‐inducible gene‐I‐like receptors and Nod‐like receptors.

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“…Finally, high-throughput small-RNA sequencing (sRNA-seq) analysis of the gill tissue of Larimichthys crocea infected with Cryptocaryon irritans identified 122 differentially expressed miRNAs (DEmiRNAs) associated with the Toll-like receptor signaling pathway, RIG-I receptor signaling pathway, phagosome, and Jak-STAT signaling pathway [11]. These results prompted further investigation of miRNA functions and target genes in fish [12][13][14]. In zebrafish, dre-miR-142a-5p was shown to target the IL-6 signal transducer (il-6st), enhancing the IL-6 signaling pathway to protect the host against Staphylococcus epidermidis infection [12].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomics Reveals the microRNA-Mediated Immune Response of Large Yellow Croaker (Larimichthys crocea) to Pseudomonas plecoglossicida Infection

Chen

Zhang

Shao

et al. 2022

Fishes

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Visceral white nodules disease (VWND), caused by Pseudomonas plecoglossicida, is a common disease among cage-farmed large yellow croaker (Larimichthys crocea) in China. However, comprehensive investigations of the molecular defensive mechanisms used by L. crocea in response to P. plecoglossicida infection remain relatively rare. Here, we constructed transcriptomes of the L. crocea spleen at 12 h and 24 h after P. plecoglossicida challenge. We identified 518 novel miRNAs and 823 known miRNAs in the spleen of L. crocea. Between the challenge and control groups, 32 differentially expressed miRNAs (DEmiRNAs), predicted to target 356 genes, and 1152 differentially expressed mRNAs (DEmRNAs) were identified at 12 h post-infection, while 33 DEmiRNAs, predicted to target 278 genes, and 1067 DEmRNAs were identified at 24 h post-infection. Gene ontology (GO) analysis showed that 146 and 126 GO terms were significantly enriched in the target genes at 12 h and 24 h, respectively. Twenty-eight and four immune-associated Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched in the target genes at 12 h and 24 h, respectively. Three immune-associated pathways were among those most enriched in the target genes: Toll-like receptor signaling, endocytosis, and C-type lectin receptor signaling. Network analysis identified 47 DEmRNA-DEmiRNA pairs. In particular, the immune-related genes TLR5S and PIGR were targeted by the miRNAs lcr-miR-7132c and dre-miR-183-5p, respectively. Dual-luciferase assays verified that lcr-miR-7132c downregulated TLR5S, suggesting that this miRNA may participate in regulating the immune response of L. crocea to P. plecoglossicida infection through the TLR5S-mediated signaling pathway. Our results help to clarify the miRNA-mediated immune response of L. crocea to P. plecoglossicida infection.

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Effect of miR-155 as a molecular adjuvant of DNA vaccine against VHSV in olive flounder (Paralichthys olivaceus)

Cited by 12 publications

References 35 publications

MicroRNA regulation of viral replication in teleost fish: a review

MicroRNA regulation of viral replication in teleost fish: a review

MicroRNA regulation of Toll‐like receptor, RIG‐I‐like receptor and Nod‐like receptor pathways in teleost fish

Comparative Transcriptomics Reveals the microRNA-Mediated Immune Response of Large Yellow Croaker (Larimichthys crocea) to Pseudomonas plecoglossicida Infection

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