Effect of Mn2+, Co2+ and H2O2 on biomass and lipids of the green microalga Chlorella vulgaris as a potential candidate for biodiesel production

Battah, Mohammed; El-Ayoty, Yassin; Abomohra, Abd El‐Fatah; El-Ghany, Salah Abd; Esmael, Ahmed

doi:10.1007/s13213-014-0846-7

Cited by 77 publications

(35 citation statements)

References 47 publications

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“…Previous studies have demonstrated that lipid accumulation in microalgae could be modified by various growth conditions other than the EDTA and P. The biomass yield and lipid accumulation in microalgal cells were also enhanced by nitrogen [5,8,9,38], sulphur limitation [39,40], silicon deficiency [12], high salinity [4,29,34], calcium [34,41], magnesium [34,42], and heavy metal such as cadmium [ 15,38] and iron [43]. It has also been found that some microelement and superoxide radical, namely, manganese, cobalt, and hydrogen peroxide, can induced lipid biosynthesis in microalgae [6]. Very few reports were available on biomass and lipid accumulation in Chlorella ellipsoidea and Chlorococcum infusionum.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Satpati

Gorain

Pal

2016

Advances in Biology

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Chlorella ellipsoidea and Chlorococcum infusionum, promising microalgae for biodiesel feedstock production, were treated with ethylenediaminetetraacetate (EDTA) and phosphorous to induce stress which was then followed by flow cytometry to study the enhanced intracellular neutral lipid content. Treatment resulted in up to a threefold increase in total lipid content of Chlorella (41.8±1.9% at 16 days of incubation period) and more than twofold increases in Chlorococcum (31.3±1.0% at 18 days of incubation period) under phosphorous starvation in the culture. It was observed that maximum biomass yields in Chlorella and Chlorococcum were 1.56±0.06 and 2.17±0.12 g/L at 1.5 g/L of phosphorous after 20 and 18 days of incubation periods, respectively. The qualitative analyses of neutral lipid bodies under stress conditions were performed by confocal microscopy and revealed bright golden-yellow lipid droplets in stress exposed cells. Significant increase of monounsaturated fatty acids under the nutrient limited conditions was suitable to produce biodiesel. The maximum biomass (g/L) and lipid content (% dry cell weight) at different stresses showed significant results (p<0.05) by single-factor Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT).

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Section: Discussionmentioning

confidence: 99%

“…Additionally, some conditions of cultivation should be strategically changed to enhance microalgal lipid. Most recent studies have found that the lipid accumulation in microalgae could be increased through altering parameters such as temperature, light, CO 2 , salinity, and nutrient concentration [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Satpati

Gorain

Pal

2016

Advances in Biology

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“…In fact, there are just a few studies using chemical oxidants as stress to microalgae. An example is a study by Battah et al (2015) where hydrogen peroxide was used as an oxidative stress to Chlorella vulgaris. The authors obtained a 29% significant increase in the lipid productivity when 4 mM hydrogen peroxide was present in the culture medium.…”

Section: Nannochloropsis Epa and Chemical Oxidantsmentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

131

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Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

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“…The cell densities of the cultures were determined by plotting a calibration curve of OD readings against various cell numbers as previously described in [71].…”

Section: Determination Of Growth Of C Pyrenoidosa Zf Culturesmentioning

confidence: 99%

The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

Ahmed

Lin

et al. 2017

Energies

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Abstract:In the present study, eight kinds plant growth regulators-salicylic acid (SA), 1-naphthaleneacetic acid (NAA), gibberellic acid (GA 3 ), 6-benzylaminopurine (6-BA), 2, 4-epi-brassinolide (EBR), abscisic acid (ABA), ethephon (ETH), and spermidine (SPD)-were used to investigate the impact on microalgal biomass, lipid, total soluble protein, carotenoids, and polyunsaturated fatty acids (PUFAS) production of Chlorella pyrenoidosa ZF strain. The results showed the quickest biomass enhancement was induced by 50 mg·L −1 NAA, with a 6.3-fold increase over the control; the highest protein content was increased by 0.005 mg·L −1 ETH, which produced 3.5-fold over the control; total carotenoids content was induced most effectively by 1 mg·L −1 NAA with 3.6-fold higher production than the control; the most efficient elicitor for lipid production was 5 mg·L −1 GA 3 at 1.9-fold of the control; 0.2 mg·L −1 ETH induced the abundant production of 1.82 ± 0.23% linoleic acid; 0.65 ± 0.01% linolenic acid was induced by 1 mg·L −1 NAA; 2.53 ± 0.15% arachidonic acid and 0.44 ± 0.05% docosahexaenoic acid were induced by 5 mg·L −1 GA 3 . Transcriptional expression levels of seven lipid-related genes, including ACP, BC, FAD, FATA, KAS, MCTK, and SAD, were studied by real-time RT-q-PCR. 5 mg·L −1 GA 3 was the most effective regulator for transcriptional expressions of these seven genes, producing 23-fold ACP, 31-fold BC, 25-fold FAD, 6-fold KAS, 12-fold MCTK compared with the controls, respectively.

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Effect of Mn2+, Co2+ and H2O2 on biomass and lipids of the green microalga Chlorella vulgaris as a potential candidate for biodiesel production

Cited by 77 publications

References 47 publications

Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

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