Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC

Abstract

The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C18 column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF4] enabled the optimum separation of the four target compounds.

Eco‐friendly separation of catechins using cyclodextrins as mobile phase additives in RP‐HPLC

“…In other words, the changeable retention order of the catechins is due to their different sensitivities to β‐CD. Theoretically, non‐polar compounds are eluted later than polar compounds in RP‐HPLC (Li et al ., ). So, as seen in Table , the order is EGC, C, EGCG, EC and CG without additives.…”

Simultaneous Identification and Quantification of Hydroquinone, Tretinoin and Betamethasone in Cosmetic Products by Isocratic Reversed Phase High Performance Liquid Chromatography

“…pH was adjusted to pH 5 by adding glacial acetic acid P to generates sharper peak. Selection of glacial acetic acid P as a pH modifier due to its better performance than other types of acids and is often used with acetonitrile and methanol [20]. In addition, glacial acetic acid resulted in better efficiency to increase the resolution between three compounds and sharpen the peak [20].…”

Structure–Performance Correlation of Nanocellulose‐Based Polymer Electrolytes for Efficient Quasi‐solid DSSCs

“…Fortunately, the single‐peak feature of the recovery spectrum allowed us to analyze the resolution using resolution Eq. (4) [30, 31]. For ease of calculation, the half‐peak widths were chosen instead of the peak widths in Eq.…”