Effect of Mobile Phase Components on the Separation of Polypeptides Using Carbon Dioxide-Based Mobile Phases

Blackwell, John A.; Stringham, Rodger W.

doi:10.1002/(sici)1521-4168(19990201)22:2<74::aid-jhrc74>3.0.co;2-9

Cited by 29 publications

(14 citation statements)

References 10 publications

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“…3C. Although the beneficial effects of acidic and/or basic additives on enantioselectivity in SFC is well documented with other CSPs, 19,31,32 its role in the enantiorecognition process on this CSP is not clearly understood at this stage. It is likely that the trifluoroacetic acid is contributing to greater ionisation of the ristocetin A functional groups resulting in higher resolution values.…”

Section: Ristocetin a Csp Using Packed Column Sfcmentioning

confidence: 96%

Enantioselective supercritical fluid chromatography using Ristocetin A chiral stationary phases

Svensson

Owens

2000

Analyst

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Racemic mixtures of five acidic drugs have been successfully separated by supercritical fluid chromatography (SFC) using macrocyclic antibiotic chiral stationary phases (CSPs). A ristocetin A CSP has been prepared 'in-house' and effectively applied in packed capillary SFC to separate the enantiomers of dichlorprop (R(s) = 1.4), ketoprofen (R(s) = 0.9) and warfarin (R(s) = 0.9). The commercial ristocetin A CSP (Chirobiotic R) was subsequently studied in packed column SFC with similar results where the enantiomers of warfarin (R(s) = 2.2), coumachlor (R(s) = 2.5) and thalidomide (R(s) = 0.6) were separated. Interestingly, differences were observed between the two differently immobilised CSPs where the enantiomers of dichlorprop and ketoprofen, which were separated on the 'in-house' CSP, could not be separated on the commercial phase.

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Section: Ristocetin a Csp Using Packed Column Sfcmentioning

confidence: 96%

Enantioselective supercritical fluid chromatography using Ristocetin A chiral stationary phases

Svensson

Owens

2000

Analyst

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“…Blackwell and Stringham (1999) found that elution of polypeptides in SFC depended on the acidity of the mobile phase additive. According to their study, a strong acid was needed for elution and improved peak shape.…”

Section: Sfc Applications For Active Pharmaceuticals and Metabolitesmentioning

confidence: 99%

Supercritical Fluid Chromatography

Dai

Zhang

Wang‐Iverson

et al. 2012

ADME‐Enabling Technologies in Drug Design and Development

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“…In addition, SFC has demonstrated better performance and enantioresolution in chiral separations. ,, However, much less has been published on SFC separations of large biomolecules. Moreover, these papers most often concern the separations of smaller peptides rather than proteins. − The challenge in analysis of biomolecules is to solubilize the analyte in a solvent compatible with a SFC system and to maintain the solubility throughout the run. Failure to achieve these criteria can lead to inadequate retention and peak shapes, carry-overs, and reduced recovery.…”

mentioning

confidence: 99%

Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography–Mass Spectrometry Analysis of Polypeptide

2020

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The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography–mass spectrometry (SFC–MS). We demonstrate for the first time the SFC–MS separation of a mixture of polypeptides carried out using poly(styrene-co-divinylbenzene) monolithic columns and carbon dioxide/methanol mobile phase. A gradient from 2 to 40% methanol modifier containing 0.1% TFA as an acidic additive was applied for the optimized elution and the separation was achieved in less than 3 min. Selected ion monitoring enabled detection of selected masses characteristic of three ionophoric pentadecapeptide antibiotics gramicidin A, B, and C and their two corresponding isoforms. Furthermore, their identity was confirmed through determination of their [M + 2H]2+, [M + 2Na]2+, and [M + H + Na]2+ ions acquired by positive-ion electrospray ionization-mass spectrometry (ESI-MS).

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Effect of Mobile Phase Components on the Separation of Polypeptides Using Carbon Dioxide-Based Mobile Phases

Cited by 29 publications

References 10 publications

Enantioselective supercritical fluid chromatography using Ristocetin A chiral stationary phases

Enantioselective supercritical fluid chromatography using Ristocetin A chiral stationary phases

Supercritical Fluid Chromatography

Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography–Mass Spectrometry Analysis of Polypeptide

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