Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Dainous, Francine; Kanfer, Julian N.

doi:10.1007/978-1-4684-5212-9_12

Cited by 2 publications

(2 citation statements)

References 25 publications

(21 reference statements)

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“…The partial phospholipid composition for rat brain aggregating cultures containing a large number of neurons has been reported (Dainous and Kanfer, 1986). In these cells, the PtdEtn accounted for 21.6 * 0.9% of the total phospholipids compared to 25.9% in the mixed spinal cord cultures.…”

Section: Discussionmentioning

confidence: 98%

Phospholipid and phospholipid fatty acid composition of mixed murine spinal cord neuronal cultures

Murphy

Anderson

Horrocks

1993

J of Neuroscience Research

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The phospholipid and phospholipid fatty acid compositions of mixed murine spinal cord neuronal cultures are reported. The phospholipid composition was primarily comprised of ethanolamine glycerophospholipids (44.8%) and choline glycerophospholipids (43.5%). Plasmalogens made up 29.1% of the ethanolamine glycerophospholipids (13.0% of the total phospholipids) and 4.5% of the choline glycerophospholipids (1.9% of the total phospholipids). Other phospholipids ranged from 2.9% for sphingomyelin to 1.0% for phosphatidylinositol 4-phosphate. The fatty acid compositions of the ethanolamine glycerophospholipids, choline glycerophospholipids, phosphatidylserine, and phosphatidylinositol were also determined. The choline glycerophospholipids were the most saturated and contained the smallest amount of polyunsaturated fatty acids. The ethanolamine glycerophospholipids were the most unsaturated and contained the highest amount of polyunsaturated fatty acids. The phospholipids contained minimal amounts of 20:3 n-9 (Mead acid) and are not considered polyunsaturated fatty acid deficient. Thus, for the mixed neuronal spinal cord cultures, the phospholipid fatty acid compositions were not polyunsaturated fatty acid deficient and contained a large amount of polyenoic fatty acids of both the n-3 and n-6 series.

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Section: Discussionmentioning

confidence: 98%

Phospholipid and phospholipid fatty acid composition of mixed murine spinal cord neuronal cultures

Murphy

Anderson

Horrocks

1993

J of Neuroscience Research

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“…Treatment with the same concentration of Cho instead did not increase the relative proportion of CPG (35%) but rather maintained it under steady-state levels, most likely because EPG synthesis was not impaired. Enrichment of the dEPG analogue was noticed also in C6 glioma cells (McKenzie and Brophy, 1984;Volpe et al, 1986) and fetal rat brain aggregating cell cultures (Dainous and Kanfer, 1986), suggesting that dEa is a universal, although not a common, precursor.…”

Section: Figmentioning

confidence: 97%

N‐Methyl Bases of Ethanolamine Prevent Apoptotic Cell Death Induced by Oxidative Stress in Cells of Oligodendroglia Origin

Brand

Gil

Yavin

2000

Journal of Neurochemistry

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A major reason for brain tissue vulnerability to oxidative damage is the high content of polyunsaturated fatty acids (PUFAs). Oligodendroglia-like OLN 93 cells lack PUFAs and are relatively insensitive to oxidative stress. When grown in serum-free defined medium in the presence of 0.1 mM docosahexaenoic acid (DHA; 22:6 n-3) for 3 days, OLN 93 cells release in the medium 2.6-fold more thiobarbituric acid-reactive substances ( TBARS) after a 30-min exposure to 0.1 mM H 2 O 2 and 50 M Fe 2ϩ . Release of TBARS was substantially decreased by ϳ20 and 30% on coincubation with either 1 mM N-monomethylethanolamine or N,NЈ-dimethylethanolamine (dEa), respectively. The protective effect of dEa was concentration-and time-dependent and was still visible after dEa removal, suggesting a long-lasting mechanism of protection. After 24 h following H 2 O 2 -induced stress, cell death monitored by cell sorting showed 16% of the cells in the sub-G 1 area, indicative of apoptotic cell death. DHA-supplemented cultures showed 35% cell death, whereas cosupplements with dEa reduced cell death to 12%, indicating cell rescue. Although the exact mechanism for this protection is not known, the nature of the polar head group and the degree of unsaturation may determine the ultimate resistance of nerve cells to oxidative stress.

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Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Abstract: The effect of the presence of nitrogenous bases in the growth medium of fetal rat brain aggregating cell cultures was investigated. The presence of either Nmethylethanolamine (MME) o r N,N-dimethylethanol-

Cited by 2 publications

References 25 publications

Phospholipid and phospholipid fatty acid composition of mixed murine spinal cord neuronal cultures

Phospholipid and phospholipid fatty acid composition of mixed murine spinal cord neuronal cultures

N‐Methyl Bases of Ethanolamine Prevent Apoptotic Cell Death Induced by Oxidative Stress in Cells of Oligodendroglia Origin

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