Effect of modification of membrane phospholipid composition on the activity of phosphatidylethanolamine N-methyltransferase of Tetrahymena

Smith, Joseph D.

doi:10.1016/0003-9861(83)90585-4

Cited by 15 publications

(1 citation statement)

References 34 publications

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“…Attempts to alter the lipids of cultured animal cells have been carried out by supplementing the g!owth medium with either fatty acids (Sundler and Akesson, 1974;Engelhard et al, 1976;Robert et al, 1977) or analogues of ethanolamine (EA) and choline (Chj (Glaser et al, 1974;Smith, 1983;Kaiho and Mizuno, 1984;Yavin, 1985). This approach has been used for modification of the phospholipid composition in yeast (Ratcliffe et al, 1973), protozoa (Smith, 1983), Friend cells (Kaiho and Mizuno, 1984), rat hepdtocytes (Akesson, 19771, neuroblastoma cells (Yavin, 1977j, M, cells (Honma et al, 1982, LM cells (Gilmore et al, 1979;Schroeder, 19821, and monolayer nerve cell cultures (Robert et al, 1978;Yavin, 1985). Changes o f t h e polar moiety of phospholipids can affect the phase transition temperature (Boyle and Dean, 1982) and fluidity of cell membranes (Gilmore et al, 1979;Hirata and Axelrod, 1980;Schroeder, 1982).…”

Section: Modifications Of Membrane Lipid Compositionmentioning

confidence: 99%

Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Dainous¹,

Kanfer²

1986

Enzymes of Lipid Metabolism II

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Section: Modifications Of Membrane Lipid Compositionmentioning

confidence: 99%

Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Dainous¹,

Kanfer²

1986

Enzymes of Lipid Metabolism II

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Modification of Cellular Phospholipid Composition and Consequences for Membrane Structure and Function

Smith

1993

New Developments in Lipid—Protein Interactions and Receptor Function

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Partial purification and kinetic characterization of the microsomal phospholipase A2 from thermally acclimated rainbow trout (Salmo garidneri)

Neas

Hazel

1985

J Comp Physiol B

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Phospholipase A2 (PLA2) was extracted from liver microsomal membranes of both 5 and 20 degrees C-acclimated rainbow trout (Salmo gairdneri), using the non-ionic detergent, Triton X-100. Further purification was achieved by precipitation with 35-65% ammonium sulfate followed by gel filtration chromatography in the presence of 0.1% Triton X-100 on Sephadex G-200. These procedures resulted in a 30-fold purification and the removal of all traces of phospholipid from the enzyme of both warm- and cold-acclimated trout. Column elution profiles were similar for both acclimation groups, yielding a molecular weight estimate for the trout liver enzyme of 73,000. Comparisons of activity levels and kinetic parameters of PLA2 from warm- and cold-acclimated fish indicated that compensation for temperature at non-saturating substrate concentrations was an attribute of both the particulate (microsomal) enzyme and the lipid-free protein. Cold acclimation resulted in higher activity below Vmax due primarily to decreased apparent Km values. These adaptations to temperature could not be attributed to the interaction of the enzyme with the membrane lipids, but were due to qualitative changes in the enzyme that resulted from acclimation. Other adaptive qualities of PLA2, such as reduced Km in response to acute decreases in temperature in warm-acclimated fish, were only apparent in particulate preparations, and thus were a function of the protein-lipid complex. These data suggest that an acclimation-induced increase in the activity of PLA2 may result in the activation of a deacylation-reacylation cycle at cold temperatures.

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Effect of modification of membrane phospholipid composition on the activity of phosphatidylethanolamine N-methyltransferase of Tetrahymena

Cited by 15 publications

References 34 publications

Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Effect of Modification of Membrane Phospholipid Composition on Phospholipid Methylation in Aggregating Cell Culture

Modification of Cellular Phospholipid Composition and Consequences for Membrane Structure and Function

Partial purification and kinetic characterization of the microsomal phospholipase A2 from thermally acclimated rainbow trout (Salmo garidneri)

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