Effect of molybdenum on the acute toxicity of mercuric chloride. IV. Effect of molybdenum on mercury-mediated metallothionein mRNA induction

Koizumi, Toshiaki; Saito, Seiko; Yamane, Yasuhiro

doi:10.1016/0009-2797(84)90031-0

Cited by 7 publications

(2 citation statements)

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“…Jakobsen et al [ 84 ] reported MT induction in the liver of rats implanted with cobalt-chromium-molybdenum alloys, yet the authors speculated the induction as a response to the presence of cobalt, chromium, manganese, iron, and/or nickel but not to Mo. Koizumi et al [ 85 ] demonstrated that Mo did not elevate levels of MT mRNA; however, increases in mRNA are not always concomitant with increases in protein. This study is the first to suggest that Mo does not stimulate MT protein expression.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

2015

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To determine if molybdenum (Mo) is a chemical stressor, fingerling and juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne sodium molybdate (0, 2, 20, or 1,000 mg l-1 of Mo) and components of the physiological (plasma cortisol, blood glucose, and hematocrit) and cellular (heat shock protein [hsp] 72, hsp73, and hsp90 in the liver, gills, heart, and erythrocytes and metallothionein [MT] in the liver and gills) stress responses were measured prior to initiation of exposure and at 8, 24, and 96 h. During the acute exposure, plasma cortisol, blood glucose, and hematocrit levels remained unchanged in all treatments. Heat shock protein 72 was not induced as a result of exposure and there were no detectable changes in total hsp70 (72 and 73), hsp90, and MT levels in any of the tissues relative to controls. Both fingerling and juvenile fish responded with similar lack of apparent sensitivity to Mo exposure. These experiments demonstrate that exposure to waterborne Mo of up to 1,000 mg l-1 did not activate a physiological or cellular stress response in fish. Information from this study suggests that Mo water quality guidelines for the protection of aquatic life are highly protective of freshwater fish, namely rainbow trout.

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Section: Discussionmentioning

confidence: 99%

The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

2015

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“…Molybdenum, however reduces the ability of rats to respond to temperature stress through unknown mechanism (Winston et al, 1973) Recently, we found that pretreatment of rats with Na2MoO4 protected them against the acute toxicity of CdC12 as well as against that of I-IgC12 (Yamane and Koizumi, 1982;Yamane et al, 1990). The mechanism by which molybdenum pretreatment alleviated the acute toxicity of those metals has not been explained fully, although it is evident that it, in part, involves the prevention of deregulation of inorganic cation metabolism such as cellular potassium loss and calcium increase and stimulation of metaUothionein synthesis in the liver and kidney (Yamane et al, 1990;Koizumi and Yamane, 1984;Koizumi and Yamane, 1984;Koizumi and Yamane, 1988). Considering the fact, however, that cadmium-or mercuryinduced irreversible cell damage seems to attribute to the alteration of cell membrane function such as disturbance of ion transports occurring in the liver and kidney within 2 hr after exposure to those metals (McDoweU et al, 1976;Doudley et al, 1984) and the fact that the rate of synthesis of metallothionein, being capable of detoxifying those metals by sequestration, increases to a maximum 4-8 hr after exposure to those metals Hildebrand et al, 1982), it is speculated that molybdenum pretreatment may reduce cadmiumor mercury-induced toxicity by ameliorating the harmful action of those metals on the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Protective mechanism of sodium molybdate against the acute toxicity of cadmium in rats. II. Prevention of cytoplasmic acidification

et al. 1991

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In order to clarify the protective mechanism of sodium molybdate against the acute toxicity of cadmium chloride in rat, the effect of in vivo sodium molybdate pretreatment on the cytotoxic action of cadmium in isolated hepatocytes was studied. The cytosolic pH of hepatocytes isolated from untreated rats immediately decreased with incubation in either neutral Hank's balanced salt solution (HBS), pH 7.4, containing 5 microM cadmium chloride minimum or acidic HBS (pH 7.1, 6.8, 6.5, and 6.2). The presence of 5 microM cadmium in HBS adjusted to pH 7.1 aggravated cytosolic acidification induced by the acidic medium alone. Cell viability of hepatocytes incubated in HBS at pH 6.2 was significantly reduced as compared to that of control cells in HBS at pH 7.4, but the presence of cadmium in the acidic HBS had no aggravating action against such a toxic action of the acidic medium although cellular uptake of the metal in the medium increased, as compared to that in HBS at pH 7.4. Molybdenum pretreatment alleviated cytoplasmic acidification induced by the treatment with HBS at pH 7.4 or 7.1 containing cadmium or by extracellular acid load without cadmium. This pretreatment also prevented the loss of cell viability induced by the treatment with HBS at pH 6.2 but could not attenuate that when cadmium was present in the medium. These facts suggest that molybdenum pretreatment alleviated the acute toxicity of cadmium in rat by preventing cytoplasmic acidification caused by the harmful metal.

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Molybdenum

Parker

1986

Anthropogenic Compounds

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Effect of molybdenum on the acute toxicity of mercuric chloride. IV. Effect of molybdenum on mercury-mediated metallothionein mRNA induction

Cited by 7 publications

References 42 publications

The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

Protective mechanism of sodium molybdate against the acute toxicity of cadmium in rats. II. Prevention of cytoplasmic acidification

Molybdenum

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