The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

Ricketts, Chelsea D.; Bates, William; Reid, Scott D.

doi:10.1371/journal.pone.0115334

Cited by 9 publications

(7 citation statements)

References 80 publications

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“…Molybdenum is an important micronutrient because it acts as a catalytic centre for more than 50 enzymes (Ricketts et al, 2015). Molybdenum was eluted in two clear peaks (Fig.…”

Section: Molybdenummentioning

confidence: 99%

Cytosolic distributions of highly toxic metals Cd and Tl and several essential elements in the liver of brown trout (Salmo trutta L.) analyzed by size exclusion chromatography and inductively coupled plasma mass spectrometry

et al. 2018

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Cytosolic distributions of nonessential metals Cd and Tl and seven essential elements among compounds of different molecular masses were studied in the liver of brown trout (Salmo trutta) from the karstic Krka River in Croatia. Analyses were done by size exclusion high performance liquid chromatography and high resolution inductively coupled plasma mass spectrometry. Common feature of Cd and Tl, as highly toxic elements, was their distribution within only two narrow peaks. The increase of cytosolic Cd concentrations was reflected in marked increase of Cd elution within low molecular mass peak (maximum at ∼15 kDa), presumably containing metallothioneins (MTs), which indicated successful Cd detoxification in brown trout liver under studied exposure conditions. Contrary, the increase of cytosolic Tl concentrations was reflected in marked increase of Tl elution within high molecular mass peak (maximum at 140 kDa), which probably indicated incomplete Tl detoxification. Common feature of the majority of studied essential elements was their distribution within more peaks, often broad and not well resolved, which is consistent with their numerous physiological functions. Among observed associations of essential metals/nonmetal to proteins, the following could be singled out: Cu and Zn association to MTs, Fe association to storage protein ferritin, and Se association to compounds of very low molecular masses (<5 kDa). The obtained results present the first step towards identification of metal-binding compounds in hepatic cytosol of brown trout, and thus a significant contribution to better understanding of metal fate in the liver of that important bioindicator species.

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“…Molybdenum is an important micronutrient because it acts as a catalytic centre for more than 50 enzymes (Ricketts et al, 2015). Molybdenum was eluted in two clear peaks (Fig.…”

Section: Molybdenummentioning

confidence: 99%

Cytosolic distributions of highly toxic metals Cd and Tl and several essential elements in the liver of brown trout (Salmo trutta L.) analyzed by size exclusion chromatography and inductively coupled plasma mass spectrometry

et al. 2018

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“…Possibly, the exposure to the effluent might pose constraint on the underlying variability in the real-world situation (Tyler et al 1998). Since mta was not affected, heavy metal stress seems to be unlikely (Ricketts et al 2015. In previous work, we observed a clear increase in the expression of mt in response to zinc in carp liver, with no concomitant significant change of vg expression.…”

Section: Discussionmentioning

confidence: 69%

Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss)

Nieto

Leal

Schwaiger³

et al. 2021

Austral j. vet. sci.

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With the aim to characterise the biological impact of anthropogenic discharge in a river, gene expression in fish was evaluated as a biomarker for mixture effects of potential toxic compounds in the aquatic environment. Adult male rainbow trout (Oncorhynchus mykiss) were used as monitoring organisms. Within the German experimental set up, trout were exposed actively for 28 days in biologically purified sewage as well as in river water up-and downstream of the sewage treatment plant (STP) effluent. In a different approach, wild trout were captured in southern Chile near anthropogenic discharges. Fish were taken from river Maullin receiving sewage from a settlement and from river Pescado influenced by effluents from a fish farm. In vivo effects were assessed by RT-qPCR analyses of biomarker gene expression, vitellogenin (vg), metallothionein (mt) and cytochrome 1A (cyp1A) in liver samples with primers amplifying specific sequences previously confirmed by cloning and sequencing. The modulation of expression of marker genes involved in metal stress, reproduction and detoxifying systems in the liver of male rainbow trout revealed organismal response to anthropogenic contamination in two different study areas, such as Chile and Germany, thereby indicating a potential risk on the aquatic ecosystems.

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“…MOCS2 , a gene encoding the eukaryotic molybdoenzyme, has been widely reported to contribute to molybdenum cofactor synthesis [ 67 ]. For its expression pattern, this gene has been confirmed to be predominantly expressed in heart and skeletal muscle, thus contributing to the identification of muscle and heart cells [ 67 , 68 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

A Computational Method for Classifying Different Human Tissues with Quantitatively Tissue-Specific Expressed Genes

Lei

Zhang

et al. 2018

Genes

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Tissue-specific gene expression has long been recognized as a crucial key for understanding tissue development and function. Efforts have been made in the past decade to identify tissue-specific expression profiles, such as the Human Proteome Atlas and FANTOM5. However, these studies mainly focused on “qualitatively tissue-specific expressed genes” which are highly enriched in one or a group of tissues but paid less attention to “quantitatively tissue-specific expressed genes”, which are expressed in all or most tissues but with differential expression levels. In this study, we applied machine learning algorithms to build a computational method for identifying “quantitatively tissue-specific expressed genes” capable of distinguishing 25 human tissues from their expression patterns. Our results uncovered the expression of 432 genes as optimal features for tissue classification, which were obtained with a Matthews Correlation Coefficient (MCC) of more than 0.99 yielded by a support vector machine (SVM). This constructed model was superior to the SVM model using tissue enriched genes and yielded MCC of 0.985 on an independent test dataset, indicating its good generalization ability. These 432 genes were proven to be widely expressed in multiple tissues and a literature review of the top 23 genes found that most of them support their discriminating powers. As a complement to previous studies, our discovery of these quantitatively tissue-specific genes provides insights into the detailed understanding of tissue development and function.

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The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss

Cited by 9 publications

References 80 publications

Cytosolic distributions of highly toxic metals Cd and Tl and several essential elements in the liver of brown trout (Salmo trutta L.) analyzed by size exclusion chromatography and inductively coupled plasma mass spectrometry

Cytosolic distributions of highly toxic metals Cd and Tl and several essential elements in the liver of brown trout (Salmo trutta L.) analyzed by size exclusion chromatography and inductively coupled plasma mass spectrometry

Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss)

A Computational Method for Classifying Different Human Tissues with Quantitatively Tissue-Specific Expressed Genes

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