Effect of monensin withdrawal on rumen fermentation, methanogenesis and microbial populations in cattle

Abrar, Arfan; Tsukahara, Takamitsu; Kondo, Makoto; Ban‐Tokuda, Tomomi; Wang, Chao; Matsui, Hiroki

doi:10.1111/asj.12368

Cited by 6 publications

(9 citation statements)

References 22 publications

(56 reference statements)

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“…There have been a number of experiments with monensin as a rumen modifier in various production systems, where CH 4 production was studied as a main objective either from a mitigation or from an energy loss perspective. [33][34][35][36] Although some studies reported a long-term mitigating effect of monensin on CH 4 production, overall the effect of the ionophore on methane production appears to be inconsistent, transient and short-lived indicating that microbial adaptation occurs.…”

Section: Bacteriocins and Ionophoresmentioning

confidence: 99%

Use of feed additives for reducing greenhouse gas emissions from dairy farms

Kataria¹

2016

Microbiol Res (Pavia)

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This review analyses methane emissions from dairy farms due to enteric fermentations and use of different feed additives as a strategy to control them. CH4 is a product that forms during the fermentation of food in the rumen of ruminants and on average represents a 7% loss of the energy ingested by the animal. CH4 is also a potent greenhouse gas. Various approaches have been studied in many countries with the aim of reducing methane emissions of digestive origin like the use of biotechnologies to modify the microbial ecosystem. This include selection of rumen micro-organisms through the elimination of protozoans or the inoculation of exogenous bacterial strains, vaccination against methanogenic micro-organisms, etc. or use of new food additives like plant extracts, organic acids, etc. and are theoretically promising paths. Their application is however still not known because trials are being performed mainly <em>in vitro</em>. This article focuses on reducing methanogenesis by adjusting the composition of the feed distributed to animals.

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Section: Bacteriocins and Ionophoresmentioning

confidence: 99%

Use of feed additives for reducing greenhouse gas emissions from dairy farms

Kataria¹

2016

Microbiol Res (Pavia)

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“…After 24 h of incubation, all bottles were cooled on ice to stop fermentation and sampled to determine headspace gas composition, dry matter degradability, short-chain fatty acids (SCFAs), methane generation, and the gene (mcrA). Analyses were conducted as described previously (Denman et al, 2007;Abrar et al, 2015). Real-time PCR for the quantification of mcrA copy number was performed on a StepOnePlus Real-Time PCR Systems (Applied Biosystems, Foster City, CA USA).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Changes in in vitro Rumen Fermentation Characteristics of Different Compositions of Total Mixed Rations (TMR) and the Ensiled TMRs

Wang¹

2016

Adv. Anim. Vet. Sci.

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| To evaluate the effects of the composition of total mixed rations (TMR) and ensiling of the TMR on rumen fermentation properties and methane production, we compared two types of TMRs, which were optimized for dairy cattle and beef cattle, and their ensiled TMRs (eTMR). To make eTMRs, TMRs were wrapped and fermented for 40 days. These eTMRs and TMRs were used for in vitro ruminal incubation experiment. The type of TMR and ensiling both affected total short chain fatty acids, the amount of methane production, and relative proportions of acetate and butyrate in the in vitro rumen cultures of tested TMRs. The relative abundance methanogenic archaea in respective cultures determined by quantifying a gene involved in methane production (mcrA: α-subunit of methyl co-enzyme M reductase) was also affected by both the type and the ensiling, which was higher in the eTMRs than the TMRs (p<0.001). The results of the present study suggest that not only ensiling TMR but also the composition of TMR may affect in vitro rumen fermentation patterns, and that changes in the degree of methane generation due to ensiling TMR may also depend on the fermentation kinetics.

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“…MSS was supplemented at 0, 40, 80 mg/kg (dry matter of substrate); DF was added into serum bottles in vitro, and the final treatment concentrations were 0, 7, and 14 mmol/L (Table 1). The concentrations of MSS and DF were slightly adjusted according to previous studies [10,12,14,17]. Incubation substrate (450 mg) was dispensed into a 100 mL serum bottle with an appropriate amount of MSS and/or DF, and then 45 mL artificial rumen inoculum was transferred into each bottle under anaerobic conditions.…”

Section: In Vitro Rumen Incubationmentioning

confidence: 99%

“…There are two main mechanisms to effectively abate CH 4 formation this way [8]: the first is to maintain a low H 2 pressure by inhibiting H 2 liberating reactions. Monensin is an ionophore that can effectively inhibit hydrogen-producing microorganisms (Gram-positive bacteria and protozoa), resulting in more propionate, which means less acetate, formate, butyrate and hydrogen are produced in the rumen [9,10]. Monensin has been shown to decrease the methane emissions of rumen [11,12].…”

Section: Introductionmentioning

confidence: 99%

Methane Emissions Regulated by Microbial Community Response to the Addition of Monensin and Fumarate in Different Substrates

2021

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Ruminants contribute significantly to global methane (CH4) emissions. This study aimed to evaluate the in vitro effects of monensin sodium salt (MSS) and disodium fumarate (DF) on CH4 production, rumen fermentation, and microbial community, with different substrates. The addition of MSS and DF, alone and in combination, significantly reduced the concentration and production of CH4 (p < 0.05), and while with vinasse as substrate, the CH4 production was higher for forage. The highest propionate production and lowest acetate and propionic ratio (A:P) values were all observed in cultures added to the combination of 14 mmol/L DF and 80 mg/kg MSS in both substrates, suggesting that these additives improved the rumen fermentation efficiency. The diversity indexes of prokaryotic microbiota with forage as the substrate were significantly higher than vinasse, and there were different effects on diversity indexes with the addition of MSS and DF depending on the incubated substrate. Supplementation with MSS and DF increased the number of starch degradation and fumarate reducing bacteria, decreased the number of methanogens, but had no significant effect on the number of fibrolytic bacteria. pH, NH3-N, and rumen volatile fatty acids (VFA) were the main factors influencing prokaryotic community structure. In conclusion, basal substrates (forage and vinasse) and CH4 mitigation additives (MSS and DF) have interactions on the in vitro rumen fermentation and microbial composition.

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Effect of monensin withdrawal on rumen fermentation, methanogenesis and microbial populations in cattle

Cited by 6 publications

References 22 publications

Use of feed additives for reducing greenhouse gas emissions from dairy farms

Use of feed additives for reducing greenhouse gas emissions from dairy farms

Changes in in vitro Rumen Fermentation Characteristics of Different Compositions of Total Mixed Rations (TMR) and the Ensiled TMRs

Methane Emissions Regulated by Microbial Community Response to the Addition of Monensin and Fumarate in Different Substrates

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