This study investigated the effect of fumarate (FUM) and rice bran (RB), alone and together, on in vitro rumen fermentation, methanogenesis and methanogens. In vitro incubation was performed with six media that were either unsupplemented (control) or supplemented with 10% RB, 5 mmol/L FUM, 10% RB + 5 mmol/L FUM, 10 mmol/L FUM, or 10% RB + 10 mmol/L FUM. Methane (CH4 ) production, dry matter digestibility, CH4 per digested dry matter, total short-chain fatty acid (SCFA) production, proportion of SCFA, acetate : proprionate ratio, production of NH3 -N, and population density of rumen microbes were determined. Supplementation with 10% RB + 10 mmol/L FUM yielded a 36% decrease in CH4 production compared to the control. Supplementation of FUM, in the presence or absence of RB, provided increases in total SCFA production and propionate proportion up to 61% and 31%, respectively. Total bacteria, methanogens and protozoa populations were significantly (P < 0.05) decreased with the 10% RB + 10 mmol/L FUM supplementation. The effect of anti-methanogenesis of FUM was enhanced by the addition of RB. Notably, the CH4 production attenuation was achieved by 10% RB + 10 mmol/L FUM without reduction of digestibility or of ruminal fermentation.

The objective of the research was to evaluate and characterize of a Lactic Acid Bacteria isolated frombroiler's small intestine. The research was done by three steps : (1) Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria. The isolation of lactic acid bacteria was proceed by clone from broiler's small intestine with MRS Broth for 24 hours, thinned until 10 ̄ 9 with serial number, invested in MRS jell, purify by streak plate method and storage the lactic acid bacteria in an MRS angle jell. Gram colourize was done with crystal violet liquid, Iodium, Alkohol 95% and Safranin, and also microscopic observation was done by electron microscope with 40 zooming. Inhibitory test with pathogen bacteria was evaluated with Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 dan 48 hours incubation period.The isolation result showed two sample of isolate (A1 dan A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate A1 the colonies had coccus and white colour. The isolate of A2 had sarcinar. After that, gram colouring was done and microscopic observation were identified into gram-negative bacteria became bacill and bacill cocco. Inhibitory test with pathogen bacteria showed that it produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactobacillus sp as the genera of Lactic Acid Bacteria.

This study was designed to obtain information on the residual influence of dietary monensin on ruminant fermentation, methanogenesis and bacterial population. Three ruminally cannulated crossbreed heifers (14 months old, 363 ± 11 kg) were fed Italian ryegrass straw and concentrate supplemented with monensin for 21 days before sampling. Rumen fluid samples were collected for analysis of short chain fatty acid (SCFA) profiles, monensin concentration, methanogens and rumen bacterial density. Post-feeding rumen fluid was also collected to determine in vitro gas production. Monensin was eliminated from the rumen fluid within 3 days. The composition of SCFA varied after elimination of monensin, while total production of SCFA was 1.78 times higher than on the first day. Methane production increased 7 days after monensin administration ceased, whereas hydrogen production decreased. The methanogens and rumen bacterial copy numbers were unaffected by the withdrawal of monensin.

The purpose of this study was to investigate the diversity and fluctuation in the ciliate protozoan population in the rumen of cattle. DNA was extracted from the rumen of three ruminally cannulated, crossbred cattle and a polymerase chain reaction (PCR)-derived clone library was constructed, using a specific primer set targeting 18S ribosomal RNA genes of ciliate protozoa. DNA fragments of seven selected clones were validated for standard DNA of the protozoa-specific real-time PCR assay. Furthermore, population fluctuation of ciliate protozoa and methanogens in the cattle rumen was determined by real-time PCR. A total of 60 clones were sequenced, phylogenetically analyzed, and classified into 24 operational taxonomic units (OTUs) based on a 99% similarity criterion. More than 80% sequences were phylogenetically placed in the genus Entodinium. The rest of the sequences were placed in the genus Diploplastron (5%), Dasytricha (8.3%) and Isotricha (3.3%). The results suggest that Entodinium was the dominant group in the rumen of cattle used in this study. The ciliate protozoan population showed no significant change in numbers during the monitoring period and reached a peak at 3 h after feeding. Changes in the protozoa population were lower than those of the methanogens.

Penelitian ini dilakukan untuk mempelajari aktivitas proporsi berbagai cairan rumen dalam mengatasi tannin melalui teknik in vitro. Penelitian ini dilakukan selama 2 bulan di Laboratorium Nutrisi dan Makanan Ternak Fakultas Pertanian Universitas Sriwijaya. Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan 3 proporsi cairan rumen dari berbagai ternak ruminansia (sapi, kambing, dan kerbau). Perlakuan terdiri atas P1 (25:50:25); P2 (25:25:50) dan P3 (50:25:25). Masing-masing perlakuan diulang sebanyak 4 kali. Parameter yang diamati adalah total populasi bakteri (cfu/ml), konsentrasi tannin (mg/ml), dan konsentrasi N-NH3 (mM). Semua perlakuan menunjukkan hasil yang tidak berbeda nyata, akan tetapi total populasi bakteri meningkat pada setiap perlakuan. Konsentrasi tannin tidak berbeda nyata kecuali untuk P3 (0.078 mg/ml menjadi 0.060 mg/ml). N-NH3 pada semua perlakuan tidak berbeda nyata (0.05 mM), akan tetapi ada aktivitas mikroba pada semua perlakuan. Dapat disimpulkan bahwa terdapat bakteri di berbagai cairan rumen yang mampu mencerna dan toleran terhadap tannin.Kata kunci : Aktifitas proporsi, berbagai cairan rumen, tannin, teknik in vitro