2015
DOI: 10.1111/asj.12431
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Effect of supplementation of rice bran and fumarate alone or in combination on in vitro rumen fermentation, methanogenesis and methanogens

Abstract: This study investigated the effect of fumarate (FUM) and rice bran (RB), alone and together, on in vitro rumen fermentation, methanogenesis and methanogens. In vitro incubation was performed with six media that were either unsupplemented (control) or supplemented with 10% RB, 5 mmol/L FUM, 10% RB + 5 mmol/L FUM, 10 mmol/L FUM, or 10% RB + 10 mmol/L FUM. Methane (CH4 ) production, dry matter digestibility, CH4 per digested dry matter, total short-chain fatty acid (SCFA) production, proportion of SCFA, acetate :… Show more

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Cited by 18 publications
(14 citation statements)
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“…Headspace gas production was determined by cumulating periodical gas removal. Proportion of methane was analyzed by a gas chromatography system (Shimadzu GC-8A) following previous reports [16], using a steel column (2 m, 3 mm, Shincarbon-ST; Shinwa Chemical Industries Ltd., Kyoto, Japan) and a thermal conductivity detector (TCD, 210 • C). Argon gas was used as the carrier gas at a 50 mL /min flow rate.…”
Section: Experiments 2 Batch Culture Trialmentioning
confidence: 99%
“…Headspace gas production was determined by cumulating periodical gas removal. Proportion of methane was analyzed by a gas chromatography system (Shimadzu GC-8A) following previous reports [16], using a steel column (2 m, 3 mm, Shincarbon-ST; Shinwa Chemical Industries Ltd., Kyoto, Japan) and a thermal conductivity detector (TCD, 210 • C). Argon gas was used as the carrier gas at a 50 mL /min flow rate.…”
Section: Experiments 2 Batch Culture Trialmentioning
confidence: 99%
“…SCFAs in culture fluid were analysed by high-performance liquid chromatography (HPLC) as described by Uddin et al (2010). NH 3 -N in the fluid was determined using the phenolhypochlorite method as described by Abrar et al (2016).…”
Section: In Vitro Incubationmentioning
confidence: 99%
“…Rice bran contains approximately 9.5-12.1% crude protein, 10.6-23.5% crude fiber, and 3165-3563 kcal/kg gross energy (Supriyati et al, 2015;Hardini, 2010;Warren & Farrell, 1990;Oliveira et al, 2011). Several studies have been carried out to evaluate rice bran as cattle feed (Abrar et al, 2016;Wakabayashi et al, 2013;Gadberry et al, 2004).…”
Section: Introductionmentioning
confidence: 99%