Effect of monogastric and ruminant gastrointestinal conditions onin vitroaflatoxin B₁adsorption ability by a montmorillonite

Abstract

The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of aflatoxin B₁ adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant animals. Sodium bentonite showed a high aflatoxin B₁ affinity with all of the assays. Langmuir or sigmoid isotherms were found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B₁ maximum adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B₁, different substances present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B₁ adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.

Cross-linked chitosan polymers as generic adsorbents for simultaneous adsorption of multiple mycotoxins

“…The results indicated that the presence of gastrointestinal components decreased the adsorption capability of adsorbent for coexisted multiple mycotoxins. This was similar to a previous study (Magnoli, Alonso, Cavaglieri, Dalcero, & Chiacchiera, 2013), where the adsorption capability of sodium bentonite for AFB 1 was decreased by the rumen fluid components. In spite of this, the synthetic adsorbent still presented high adsorption capability for the simultaneous presence of OTA, ZEN and FB 1 (removal percentages, >50%).…”

Negligible effects of tryptophan on the aflatoxin adsorption of sodium bentonite

“…This displacement is probably the main process responsible for the adsorption in the salt saturated interface. A decrease in  max under ionic strength control was previously also observed for AFB 1 adsorption (Magnoli et al 2013). Tessis et al, (1999); Franchi et al, (2003); Gao et al, (2008) have shown that Na + , Ca 2+ , and Mg 2+ ions affect the adsorption of biomolecules (ATP, nucleic acids, amino acids) on montmorillonite among other minerals.…”

Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1adsorbents

“…,b; Magnoli et al . , ). They were composed of bentonites, lactic acid bacteria, clay, clay plus live yeast cells, clay plus dried yeast cells, yeast cell wall components, and have demonstrated different efficiencies in reducing AFs among them (Ramos et al .…”

“…has been extensively studied in recent years (Magnoli et al . ). Although these products are widely and commercially available as feed additives for mycotoxin binding, they need to be incorporated at high levels and have side effects on some dietary nutrients, thus reducing the nutritional value of animal diets (Zain ).…”