Effect of multi-enzymes supplementation on growth performance, meat quality, ileal digestibility, digestive enzyme activity and caecal microbiota in broilers fed low-metabolizable energy diet

Abstract

Objectives: This study was conducted to evaluate the effect of using low energy diet with multienzymes supplementation on different biological parameters in broilers.Methods: Three hundred Arbor Acres broiler chicks were randomly divided into three groups [Cont (Standard ME), L-ME (ME reduced by 50 kcal/kg without enzyme) and L-ME-MES (L-ME diet was supplemented with multi-enzymes)] with five replicates per group (20 chicks per replicate) at the start of second week. Grower and finisher diets were formulated according to breed specific guide and offered with free access in respective phase (two weeks for grower (8-21d); two weeks for finisher (22-35d)). External marker method was used to measure the nutrient digestibility. After feeding trial, fifteen birds (one birds per replicate) were selected randomly and slaughtered for samples collection. Results:The results exhibited no effect (P>0.05) of dietary treatments on all parameters of growth performance, carcass traits, relative weight of internal organs except bursa and overall parameters of thigh meat quality. Relative weight of bursa was significantly (P<0.05) higher in L-ME than Control. Multi-enzymes supplementation in low-ME diet significantly (P<0.05) improved the breast meat pH 24h, digestibility of crude protein, duodenum weight and length, jejunal morphology, counts of Lactobacillus spp. and Bifidobacterium spp., lipase and protease activities than Control. Jejunum length was increased in both L-ME and L-ME-MES treatments than that of the control (P<0.05). Breast meat cooking loss and color lightness was lower in L-ME (P<0.05) than control. A c c e p t e dA r t i c l e 3 Conclusion: It can therefore be concluded that broilers could be reared on low energy diet with supplementation of multi-enzymes without compromising the growth performance. In addition, it is beneficial for other biological parameters of broilers.

Impact of Dietary Supplementation of Probiotic or Enzymes on Carcass Traits and Intestinal Microflora of Broiler

“…The present results are also in accordance with those of several studies which reported that the relative weights of bursa and spleen for broilers were not significantly affected by multi-enzymes (Vahid et al, 2012;Metwally et al, 2020 andYaqoob et al, 2022) or probiotics supplemented (Sugiharto et al, 2018;Hidayat et al, 2020).…”

“…Our findings agree with the findings of Midilli and Tuncer (2001), who reported that the best carcass and cut up yields were recorded for the enzymes and probiotics supplemented groups of broilers compared with their control. Similarly, Yaqoob et al, (2022) found that enzymes supplementation did not significantly affect liver, gizzard, heart and giblets percentages of broilers. Moreover, Hajati (2010) showed that enzyme supplementation to broiler ' s diet increased carcass, thighs drumsticks percentage significantly (P<0.05).…”

“…Moreover, Eltrefi et al, (2017) showed that using of probiotic in diets of broilers had insignificant effects on crude protein, ether extract% in breast meat. Yaqoob et al, (2022) showed that multi-enzymes supplementation significantly (P<0.05) decreased cocking loss % of breast meat of broilers.…”

“…In addition, Tang et al, (2021) proved that probiotic had insignificant effects on dressed carcass and abdominal fat % of broilers. Also, Yaqoob et al, (2022) showed that multi-enzymes supplementation did not significantly impact on relative weights of dressed carcass, breast, thigh, drumsticks and abdominal fat for broilers.…”

Effect of Natural Multi-Enzyme Supplementation on Growth Performance, Gut Microflora, Carcass Characteristics, and Meat Quality of Broilers Reared in Tropical Climates

“…The endogenous enzymes of broiler chickens cannot adequately digest the dietary non-starch polysaccharides (NSP), and a high level of ingestion of these NSP by carbohydrases may exert anti-nutritional effects in broilers by increasing the digesta viscosity and decreasing the nutrient digestibility (Zeng et al, 2015). Exogenous enzymes are supplemented into corn-soya based poultry diets to reduce the effects of differing raw material quality, improve homogeneity and enhance nutrient digestibility (Yaqoob et al, 2022;Kutlu et al, 2019).…”