Effect of mutagenesis on germination, plant survival and pollen sterility in M<sub>1</sub>generation of in cowpea [<i>Vigna unguiculata</i>(L.) Walp]

Bind, Devmani; Dwivedi, V. K.

doi:10.5958/0976-058x.2014.01322.5

Cited by 10 publications

(12 citation statements)

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“…In comparison to the wild type, the percent germination was low in all mutant populations (M 1 and M 2 populations). Similar results were reported by Ramya et al (2014) , Bind and Dwivedi (2014) , and Uma and Salimath (2001) . Significantly reduced germination percentage due to EMS treatment has also been reported in study by Shah et al (2008) in chick pea.…”

Section: Discussionsupporting

confidence: 91%

“…These outcomes are in close agreement with the earlier reports of Nawale et al (2006) , Ugorji et al (2012) , and Dhanavel and Girija (2009) . Bind and Dwivedi (2014) also reported similar findings in cowpea. The decrease in plant survival within the M 1 population may be due to the effect of the EMS.…”

Section: Discussionsupporting

confidence: 72%

“…The percentage seed germination decrease in EMS treated populations may be resulted from physiological and acute chromosomal damage ( Gaur et al, 2003 ; Nawale et al, 2006 ). According to Bind and Dwivedi (2014) , delay in the one set of mitosis and chromosomal aberration induced enzyme activity such as catalase, lipase and hormonal activity results in reduced germination. Also, the reduction in germination may be due to the seeds absorbing the mutagen, which subsequently reaches the meristemic region and affects the germ cell ( Serrat et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Induced mutation has an effect on the lethality and germination rates, chlorophyll content, viability, and other factors adding up to the improvement on the morpho-agronomic nature of cowpea plant ( Dhanavel et al, 2008 ; Dhanavel and Girija, 2009 ). Bind and Dwivedi (2014) , in their study reported decrease in percentage germination, percentage plant survival and increase in pollen sterility in cowpea treated with doses of EMS.…”

Section: Introductionmentioning

confidence: 93%

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Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

et al. 2022

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Unique variants are desired in the development of genetically improved crops to meet farmer and market needs hence ethyl methane sulfonate (EMS) was used to induce genetic variability in cowpea (Vigna unguiculata cv. Asontem). The main objective of this research was to characterize induced variations in EMS chemically mutagenized population of cowpea (Vigna unguiculata L. Walp Var. Asontem) in the M1 and M2 generations. The optimum concentration (LD50) of EMS for generating the mutagenized population was determined by treating seeds with different concentrations of EMS (0.0, 0.2, 0.4, 0.6, and 0.8% v/v) and observing the germination count after 5 days of planting the seeds in Petri dishes. Three thousand cowpea seeds were treated with the 0.4% EMS to generate the M1 and M2 populations that were evaluated for agronomic and morphological traits with untreated seeds serving as control. Data analysis involved distribution of qualitative and quantitative traits. Germination was significantly reduced in the mutagenized population (17.8%) and compared with that of the wild type (61.6%). Percentage survival was significantly higher in wild type (98.38%) as compared with the M1 population (78.46%). Percentage germination in the M2 population (74.03%) was lower than the wild type (80%). A wide spectrum of agro-morphological abnormalities was observed in the M2 population. Wide variations and uniquely different phenotypic classes were observed in leaf color, leaf shape, growth habit, plant pigmentation, twining tendency, pod curvature, seed shape, and seed coat color. M2 individuals were widely distributed for days to flowering, number of pods per plant, number of seeds per pod, number of locules per pods, percentage seed set, pod length and number of seeds per plant. In conclusion, the EMS mutagenesis was effective in inducing the unique variations that will be useful for breeding and development of new farmer preferred varieties.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

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Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

et al. 2022

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“…Some traits had lower mean values than the control plants, while the others did not differ. Similar results in variation by comparing mean values for some quantitative traits between mutant populations and control plants in the M2 and M3 generation of lentils (Laskar and Khan 2017), in Mungbam (Dewanjee and Sarkar 2018), in cowpea (Nair and Mehta 2014;Bind and Dwivedi 2014) and in grasspea (Singh and Sadhukhan 2019) induced by gamma radiation.…”

Section: Quantitative Trait Variationssupporting

confidence: 55%

High Quantitative Trait Variability in Faba Bean Mutagenized Population for High-yielding Breeding Program in Saudi Arabia

Nurmansyah

Alghamdi

Migdadi

2021

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Background: The narrow genetic base and environmental stresses are behind the low rate of faba bean yield in the past two decades. Drought stress is one of the most destructive abiotic stresses. Using induced mutagenesis on locally adapted landrace cultivar is proposed to cope with this problem. Methods: This study was conducted on faba bean landrace cultivar of Saudi Arabia, namely Hassawi 2, treated by 25 and 50 Gray gamma radiation. The genetic diversity assessment of M2 mutant populations was based on seven quantitative traits and nine Amplified Fragment Length Polymorphism (AFLP) primer combinations. Result: A total of 3419 M2 seeds were planted, of which 2782 (81.4%) seeds germinated and 2658 plants survived. A 5 to a 10-fold range of quantitative traits studied among mutant plants compared to control plants showed high variability. The number of pods per plant and seeds per plant was a valid selection criterion for a high-yielding faba bean breeding program. Nine AFLP primer combinations generated 1079 polymorphic alleles from 88 samples that comprised mutant and control plants. Shannon index (I) and expected heterozygosity (He) were 0.337 and 0.206, respectively. The AFLP results validated high variability in M2 populations. These findings will assist faba bean breeders in developing high-yielding cultivars with drought stress tolerance.

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Optimising the dosage of ethyl methanesulphonate mutagenesis in selected wheat genotypes

OlaOlorun

Shimelis

Mathew

et al. 2019

South African Journal of Plant and Soil

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Effect of mutagenesis on germination, plant survival and pollen sterility in M₁generation of in cowpea [Vigna unguiculata(L.) Walp]

Cited by 10 publications

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Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

High Quantitative Trait Variability in Faba Bean Mutagenized Population for High-yielding Breeding Program in Saudi Arabia

Optimising the dosage of ethyl methanesulphonate mutagenesis in selected wheat genotypes

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Effect of mutagenesis on germination, plant survival and pollen sterility in M1generation of in cowpea [Vigna unguiculata(L.) Walp]

Cited by 10 publications

References 0 publications

Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants

High Quantitative Trait Variability in Faba Bean Mutagenized Population for High-yielding Breeding Program in Saudi Arabia

Optimising the dosage of ethyl methanesulphonate mutagenesis in selected wheat genotypes

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Effect of mutagenesis on germination, plant survival and pollen sterility in M₁generation of in cowpea [Vigna unguiculata(L.) Walp]