Effect of Mycoplasma Infection on Esterase Activity of Human FL Amnion and WI-38 Cells

Rose, Noel R.; Milisauskas, Vita K.; Kite, Joseph H.

doi:10.3181/00379727-140-36465

Cited by 7 publications

(2 citation statements)

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“…Twelve isozyme systems were easily detected in some or all mycoplasmas, whereas nine enzymes were not detected in extracts of 20 mycoplasmas tested ( Table 1). Of the 12 enzymes found in mycoplasmas, 9 (purine nucleoside phosphorylase, adenylate kinase, dipeptidase, esterase, glyceraldehyde-3-phosphate dehydrogenase, glucose phosphate isomerase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and superoxide dismutase) have been reported previously for selected species (16,22,32,34,43; M. Gerwitz and P. J. Van DeMark, Bacteriol.…”

Section: Discussionmentioning

confidence: 97%

Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma

O’Brien¹,

Simonson²,

Grabowski³

et al. 1981

J Bacteriol

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Crude extracts of triple-cloned, purified cultures of 22 species of Mycoplasma and Acholeplasma were examined for expression of 21 isozyme systems routinely used to type mammalian cells. Nine previously described enzymes (purine nucleoside phosphorylase, adenylate kinase, dipeptidase, esterase, glyceraldehyde-3-phosphate dehydrogenase, glucose phosphate isomerase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and superoxide dismutase) and three enzymes not previously reported in mycoplasma (triose phosphate isomerase, inorganic pyrophosphatase, and acid phosphatase) were detected in some or all of the species examined. These findings provide new information on the enzymatic expressions of these organisms. Three of the isozyme systems (superoxide dismutase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase) were present in Acholeplasma species but not in any Mycoplasma species. The characteristic pattern of electrophoretic mobility of the 12 isozyme systems also provides a useful biochemical property for identification, characterization, and classification of these mycoplasmas. Mycoplasma isozyme expression for seven of the enzymes were readily detected in various infected-cell culture lines by using either cell extracts or concentrated cell culture fluids. Mycoplasma-specific enzymes found in infected-cell extracts had the same electrophoretic mobility patterns as enzymes obtained from broth-grown mycoplasmas of the same species. Expression of homologous mammalian enzymes was not detectably altered by infection with mycoplasmas.

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Section: Discussionmentioning

confidence: 97%

Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma

O’Brien¹,

Simonson²,

Grabowski³

et al. 1981

J Bacteriol

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show abstract

“…Studies in our laboratory on a primate-specific carboxylesterase isoenzyme have led to its purification (Munjal and Rose, 1972 ;Therrien et al ., 1971) and characterization (Bartholomew et al ., 1971 ;Bartholomew et al ., 1972 ;Munjal and Rose, 1973 ;Vladutiu and Rose, 1973) . Changes in the esterase activity in cultured cells have also been studied during mycoplasmal infection (Rose et al ., 1972) . SV40 transformation (Bartholomew et al ., 1969), and in cell hybridization (Bartholomew et al ., 1971) .…”

Section: Introductionmentioning

confidence: 99%