Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites

Abstract

Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20-30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

“…Regarding bioactive glasses, particularly those of nominally Bioglass w 45S5 composition, the effects of their ionic dissolution products on the stimulation of endothelial cell proliferation, such as HUVECs, have been widely reported [13,20]. In addition, a recent work has confirmed the effects on the promotion of blood vessel growth by bioactive glass nanoparticles with the same composition as that previously mentioned, when they are incorporated in polymer-based scaffolds for BTE [81]. These considerations are in agreement with the results of our study, where the 2D scaffolds containing Nbg showed a higher proliferation of HUVECs and a better capability of forming tubes in Matrigel in comparison with those without Nbg.…”

Physical and biological characteristics of nanohydroxyapatite and bioactive glasses used for bone tissue engineering

“…Such a composite matrix, when seeded with human-like osteoblast cells, has shown better osteoconductive properties compared to monolithic HA and has resulted in calcification of an identical bone matrix [160]. In another study, Vargas et al [161] investigated the effect of nano-sized Bioglass ® 45S5 (n-BG) particles, 20-30 nm in size, on the angiogenic properties of bovine type I collagen/n-BG composites. Two concentrations of n-BG were considered in this study (i.e., 10 wt% and 20 wt%).…”

“…Two concentrations of n-BG were considered in this study (i.e., 10 wt% and 20 wt%). The addition of a limited concentration (10 wt%) of n-BG to collagen films induced an early angiogenic response, suggesting that this collagen/n-BG composition could be an attractive matrix for tissue engineering applications [161].…”

Tailoring the viscoelastic properties of injectable biocomposites: A spectroscopic assessment of the interactions between organic carriers and bioactive glass particles

“…Interestingly, so far only few investigations have been focused on the effects of the organic-BG interactions on the visco-elastic properties and structural recovery of composites. The influence of the BG particle size on the dynamic rheological changes over time (viscosity, storage and loss moduli) was recently investigated [6][7][8]. The main efforts aimed at tailoring the compositions and the textural properties of BG-organic carriers in order to improve the biomineralisation kinetics, i.e., the deposition of a surface apatite layer [9][10][11][12].…”