Effect of nano-structured bioceramic surface on osteogenic differentiation of adipose derived stem cells

Xia, Lunguo; Lin, Kaili; Jiang, Xinquan; Fang, Bing; Xu, Yinghong; Liu, Jiaqiang; Zeng, Deliang; Zhang, Maolin; Zhang, Xiuli; Chang, Jiang; Zhang, Zhiyuan

doi:10.1016/j.biomaterials.2014.06.028

Cited by 175 publications

(131 citation statements)

References 59 publications

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“…However, recent findings show that mechanotransduction is not restricted to cell surface receptors and adhesion, but can occur directly through the nucleus via phosphorylation of emerin [38]. In addition to stiffness, stem cell differentiation can be regulated by topographical cues [39][40][41][42]. Integrins also play a critical role in this process by mediating cell adhesion to micro-or nanoscale topographic features, resulting in the contraction of the cytoskeleton and activation of biochemical signalling pathways [43].…”

Section: Sensing and Integrating Mechanical Cues In Cellsmentioning

confidence: 99%

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos¹,

Reis²,

Gomes³

2015

Trends in Biotechnology

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Section: Sensing and Integrating Mechanical Cues In Cellsmentioning

confidence: 99%

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos¹,

Reis²,

Gomes³

2015

Trends in Biotechnology

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“…The three pieces of cell/ceramic constructs in each group were assessed for the cell vitality by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich) assay as previously reported. 18 Then 400 μL α-MEM supple mented with 40 μL of 5 mg/mL MTT solution was added into each well followed by sample incubation at 37°C to allow formazan formation. Four hours later, the solution with MTT was discarded and replaced by 400 μL dimethyl sulfoxide (DMSO, SigmaAldrich) to dissolve the formazan crystals.…”

Section: Mtt Assay For Cell Proliferationmentioning

confidence: 99%

“…In vitro studies showed that the nano-structured HA bioceramics could stimulate cell adhesion, proliferation, and osteogenic differentiation of BMSCs and adipose-derived stem cells (ASCs), while the mnHA achieved the best stimulatory effect. [18][19][20] However, whether the HA bioceramics with mnHA could have an enhanced effect on osteogenic and cementogenic differentiation of PDLSCs is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…42 In our previous studies, HA bioceramics with nanorod, nanosheet, and micronano-hybrid (the hybrid of nanorods and microrods) surfaces have been found to stimulate osteogenic differentiation of BMSCs and ASCs, while the mnHA possessed the best stimulation activity. 18,20 However, whether the micro/ nano-structured HA bioceramics could promote PDLSCs differentiation into periodontal tissue was not known. In this study, we aimed at investigating the effects of HA bioceramics with mnHA on viability and osteogenic and cementogenic differentiation of hPDLSCs, which might estimate the possibility of micro/nano-structured HA bioceramics for periodontal tissue regeneration.…”

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confidence: 99%

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Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Mao¹,

Liu²,

Zhao³

et al. 2015

IJN

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The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA bioceramics with flat and dense surface. Moreover, mnHA bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 ( LRP5 ) and β-catenin , which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

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“…Various studies have widely demonstrated the ability of adipose-derived stem cells (ADSCs) to differentiate into mesenchymal-derived cell lines (adipocytes, osteoblasts, chondroblasts, and endothelial cells) and not mesenchymal cell lines (skeletal muscle cells, cardiac cells, and neuronal cells) (12)(13)(14)(15). Numerous papers have been published in recent years on bone regeneration for skeletal and mandibular repair using ADSCs (16)(17)(18) …”

Section: Introductionmentioning

confidence: 99%

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

et al. 2017

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The aim of this study was to evaluate in vivo bone regeneration, mediated by adipose-derived stem cells (ADSCs), induced to differentiate into osteoblasts and carried by a scaffold gel. In the test group, bone regeneration was mediated by ADSCs, induced to differentiate into osteoblasts, and carried by a scaffold gel. In the control group a scaffold without cells was used. The scaffold, consisting of chitosan and glycerol phosphate, was maintained in situ by a cross-linked resorbable membrane. The osteogenic potential of ADSCs was confirmed by osteocalcin assay and Von Kossa staining performed before implantation. Histological assays detected an initial increase in bone formation in the test group compared with the control group. Microcomputed tomography analysis did not show significant differences between the two groups. Both histological and microcomputed tomography analysis were performed on the ex vivo specimens after a follow-up period of 8 weeks. We observed that differentiated ADSCs could increase bone regeneration and that the scaffold used here can be a suitable carrier to entrap and maintain the cells in situ. On the contrary, the membrane used was not functional in isolating the site of the defect from surrounding soft tissues and caused a significant inflammatory reaction.

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Effect of nano-structured bioceramic surface on osteogenic differentiation of adipose derived stem cells

Cited by 175 publications

References 59 publications

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

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