Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos, Lívia; Reis, Rui L.; Gomes, Manuela E.

doi:10.1016/j.tibtech.2015.06.006

Cited by 91 publications

(89 citation statements)

References 96 publications

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“…PEMF is an FDA-approved therapy presenting benefices for the treatment of several musculoskeletal disorders, however there is controversy about the role of magnetic fields in oxidative stress. Some studies suggested that magnetic fields, accounting their strength and amplitude are endowed with the ability to generate potentially harmful ROS [29], while others demonstrated that magnetic fields selectively decreased the viability of malignant cells [30], reduced oxidative stress in the brain of Gerbils [31] and activated endogenous clearance mechanisms to remove ROS [16]. We found no differences in ROS production between static and magnetic conditions suggesting that environmental stress produced by magnetically actuated biomaterials is not perceived by hASCs as harmful.…”

Section: Discussionmentioning

confidence: 49%

“…When applied, these fields alter the cell shape and conformation of the plasma membrane in inflammatory cells in a time-dependent manner [13] and influence Ca 2+ dynamics [14,15] causing changes in the cellular oxidative stress potentially influencing the inflammatory response [16]. PEMF applied in combination with magnetic responsive materials enables delivery of two distinct mechanical stimulus, one from the PEMF alone and the second from the magnetic biomaterials substrates deformed on account of magnetic actuation [17,18].…”

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confidence: 99%

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In Vitro and In Vivo Assessment of Magnetically Actuated Biomaterials and Prospects in Tendon Healing

Santos

Silva

Gonçalves

et al. 2016

Nanomedicine (Lond.)

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Section: Discussionmentioning

confidence: 49%

mentioning

confidence: 99%

In Vitro and In Vivo Assessment of Magnetically Actuated Biomaterials and Prospects in Tendon Healing

Santos

Silva

Gonçalves

et al. 2016

Nanomedicine (Lond.)

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“…Magnetic forces have been reportedly used to improve transfection rates 177 , gene therapy 178 , stem cell differentiation 179 and tissue engineering. 180 For instance, Kriha et al has previously described the production of electrospun fibers containing MNPs whose movements can be controlled to establish connections between hippocampal neurons to facilitate neural repair.…”

Section: Discussionmentioning

confidence: 99%

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Tay

2018

Springer Theses

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Neural stimulation techniques for eliciting calcium influx can elucidate the physiological roles of specific neural populations. To overcome some of the limitations of existing techniques such as poor specificity and noxious effects of heat, I developed a technology for non-invasive control of neural activities using magnetic forces and magnetic nanoparticles (MNPs) which offer deep tissue penetration and controllable dosage. Extensive investigations with different neurotoxins and experimental conditions support that the mechanism of magnetic stimulation that involves membrane-bound MNPs transducing magnetic forces into mechanical stretching of the cell membrane to enhance the opening probability of mechano-sensitive N-type calcium ion channels to induce calcium influx.Making use of the ability of neural networks to actively regulate their ratio of excitatory to inhibitory ion channel/receptor, I also performed chronic magnetic stimulation on fragile X iii syndrome (FXS) model neural networks. We found that chronic magnetic stimulation reduced the density of N-type calcium ion channels whose expression is increased in FXS. This technique demonstrates the potential of using bio-magnetic/mechanical forces to modulate expressions of mechano-sensitive ion channels where they are over-expressed in diseases such as abnormal nociception.Nonetheless, there are still a few areas where the technique can be improved. Firstly, it is the use of MNPs with more uniform properties to have greater control on magnetic stimulations.Secondly, the technique needs to be useful for in vivo studies. Therefore, I started researching on magnetotactic bacteria (MTB) which produce biological MNPs with superior properties such as uniform sizes and highly homogenous magnetic properties with the goal of harvesting MNPs from them.MTB, however, grow extremely slowly and the number of MNPs produced/bacterium is low. One way to overcome this problem is to evolve MTB over-producers of MNPs but this strategy is constrained by the absence of a selection platform that is quantitative and offer high throughput. To overcome this problem, I combined random chemical mutagenesis and selection using a magnetic ratcheting platform to generate and isolate MTB over-producers that produce twice as many MNPs/bacterium after 5 rounds of mutation/selection. I next designed a magnetic microfluidic device and demonstrate as a proof of concept, that it can be coupled to a bioreactor for high throughput microfluidic selection of MTB over-producers.iv

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“…However, when the spherical MNPs have sizes up to 100 nm, cell uptake behavior is just the opposite. [75] Thus, the versatility of MPs in terms of particle design and consequently their intrinsic characteristics as well as MPs interaction and functionality with the environment and biological entities, should be carefully considered and investigated to address a particular need and TERM application.…”

Section: Dimension Oriented Applications Of Magnetic Particlesmentioning

confidence: 99%

Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface

Silva

Babo

Costa‐Almeida

et al. 2018

Nanomedicine: Nanotechnology, Biology and Medicine

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Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Cited by 91 publications

References 96 publications

In Vitro and In Vivo Assessment of Magnetically Actuated Biomaterials and Prospects in Tendon Healing

In Vitro and In Vivo Assessment of Magnetically Actuated Biomaterials and Prospects in Tendon Healing

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface

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