Effect of nano-topographical features of Ti/TiO2 electrode surface on cell response and electrochemical stability in artificial saliva

Demetrescu, Ioana; Pîrvu, Cristian; Mitran, Valentina

doi:10.1016/j.bioelechem.2010.02.001

Cited by 85 publications

(84 citation statements)

References 45 publications

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“…It has been shown that various types of cells have dissimilar reactions to different nanotubular surfaces of disparate tube sizes. [14][15][16]18,35 Sterilization also affects the cytocompatibility of the TiO 2 surface, and ultraviolet irradiation is one of the methods to improve the bioactivity of TiO 2 without unexpected biological contamination. 35 Application of FGF2 to periodontal tissue regeneration has been previously reported.…”

Section: Discussionmentioning

confidence: 99%

“…[14][15][16][17] In addition, the TiO 2 nanotubular surface with a tube size of ∼120 nm exhibits better electrochemical stability in artificial saliva as well as improved adhesion and proliferation of human gingival fibroblasts (HGFs). 18 Fibroblast growth factors (FGFs) are a large family of polypeptide growth factors that regulate the secretion of various extracellular matrix (ECM) proteins, which contribute to the formation of new blood vessels. 19,20 Fibroblast growth factor 2 (FGF2) is a potent mitogen in a variety of cells and is effective in regulating the proliferation and ECM-related gene expression of fibroblasts, 21,22 the most common cell type in peri-implant gingival connective tissue.…”

Section: Introductionmentioning

confidence: 99%

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Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Wang²,

Chu³

et al. 2012

IJN

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Background: Titanium (Ti) implants are widely used clinically, but peri-implantitis remains one of the most common and serious complications. Healthy integration between gingival tissue and the implant surface is critical to long-term success in dental implant therapy. The objective of this study was to investigate how different concentrations of immobilized fibroblast growth factor 2 (FGF2) on the titania nanotubular surface influence the response of human gingival fibroblasts (HGFs). Methods: Pure Ti metal was anodized at 20 V to form a vertically organized titanium dioxide nanotube array on which three concentrations of FGF2 (250 ng/mL, 500 ng/mL, or 1000 ng/mL) were immobilized by repeated lyophilization. Surface topography was observed and FGF2 elution was detected using enzyme-linked immunosorbent assay. The bioactivity changes of dissolvable immobilized FGF2 were measured by methyl-thiazolyl-tetrazolium assay. Behavior of HGFs was evaluated using adhesion and methyl-thiazolyl-tetrazolium bromide assays. Results: The FGF2 remained for several days on the modified surface on which HGFs were cultured. Over 90% of the dissolvable immobilized FGF2 had been eluted by Day 9, whereas the FGF2 activity was found to diminish gradually from Day 1 to Day 9. The titania nanotubular surface with an optimal preparing concentration (500 ng/mL) of FGF2 immobilization exhibited improved HGF functions such as cellular attachment, proliferation, and extracellular matrix-related gene expression. Moreover, significant bidirectional as well as concentration-and time-dependent bioactivity was observed. Conclusion: Synergism of the FGF2-impregnated titanium dioxide nanotubular surface revealed good gingival-implant integration, indicating that these materials might have promising applications in dentistry and other biomedical devices.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Wang²,

Chu³

et al. 2012

IJN

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“…Recent reports have shown better cell attachment to nanotubular surfaces that are more hydrophobic than unmodified surfaces. 20,41,42 But according to some results in the literature, fibroblasts prefer a smooth surface to a rough surface. 43 To prevent any side effect of CNN2 in the bone healing process, a two-stage solution may be a good choice for the further clinic application of the CNN2-loaded titania nanotube surface on percutaneous implant.…”

Section: Dovepressmentioning

confidence: 99%

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Wei¹,

Wu²,

Feng³

et al. 2012

IJN

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Infection and epithelial downgrowth are major problems associated with maxillofacial percutaneous implants. These complications are mainly due to the improper closure of the implant-skin interface. Therefore, designing a percutaneous implant that better promotes the formation of a stable soft tissue biologic seal around percutaneous sites is highly desirable. Additionally, the fibroblast has been proven to play an important role in the formation of biologic seals. In this study, titania nanotubes were filled with 11.2 kDa C-terminal CCN2 (connective tissue growth factor) fragment, which could exert full CCN2 activity to increase the biological functionality of fibroblasts. This drug delivery system was fabricated on a titanium implant surface. CCN2 was loaded into anodized titania nanotubes using a simplified lyophilization method and the loading efficiency was approximately 80%. Then, the release kinetics of CCN2 from these nanotubes was investigated. Furthermore, the influence of CCN2-loaded titania nanotubes on fibroblast functionality was examined. The results revealed increased fibroblast adhesion at 0.25, 0.5, 1, 2, 4, and 24 hours, increased fibroblast viability over the course of 5 days, as well as enhanced actin cytoskeleton organization on CCN2-loaded titania nanotubes surfaces compared to uncoated, unmodified counterparts. Therefore, the results from this in vitro study demonstrate that CCN2-loaded titania nanotubes have the ability to increase fibroblast functionality and should be further studied as a method of promoting the formation of a stable soft tissue biologic seal around percutaneous sites.

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“…Hori et al have also reported the age-dependent degradation of the protein adsorption capacity of titanium 11) . Cell adhesion is an important parameter for evaluating whether implants are suitable for medical use 22) . Thus, a stable connection between the biomaterial surface and surrounding tissue is an important prerequisite for the long-term success of implants.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of H2O2 hydrothermal treatment of aged titanium surface to enhance biofunctional activity

et al. 2013

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Surface modification of titanium has been extensively investigated in implant science and technology in an effort to improve its osteoconductivity. The rate of protein adsorption on titanium surfaces is known to vary depending on the chemistry, structure, morphology, and titanium-specific biological aging of the surface. It is thus desirable to modify smooth titanium surfaces of miniimplants used as orthodontic anchors immediately prior to use. In this study, we have developed a simple surface modification of titanium alloy that improves its biofunctional activity. The surface of a Ti-6Al-4V disk was modified by applying 3% H 2 O 2 hydrothermal treatment using an autoclave. A nanostructured porous network TiO 2 was observed on the treated surface. Treated surfaces exhibited higher hydrophilicity, protein adsorption, and cell proliferation than untreated surfaces. 3% H 2 O 2 hydrothermal treatment is thought to provide biofunctional activity for aged titanium surface.

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Effect of nano-topographical features of Ti/TiO2 electrode surface on cell response and electrochemical stability in artificial saliva

Cited by 85 publications

References 45 publications

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Increased fibroblast functionality on CNN2-loaded titania nanotubes

In vitro evaluation of H2O2 hydrothermal treatment of aged titanium surface to enhance biofunctional activity

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