Effect of Nano-zirconia on Microstructure and Biological Behavior of Hydroxyapatite-Based Bone Scaffolds

Maleki-Ghaleh, H.; Khalil‐Allafi, Jafar; Keikhosravani, Pardis; Etminanfar, Mohamadreza; Behnamian, Yashar

doi:10.1007/s11665-020-04927-2

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…Calcium ions in HA positively affect MSC proliferation in culture media [44]. The doped Zn in HA further promoted this phenomenon by stimulating signaling pathways that lead to boosting cell proliferation [18,19].…”

Section: Cellular Behaviormentioning

confidence: 99%

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Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

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Bacteria are one of the significant causes of infection in the body after scaffold implantation. Effective use of nanotechnology to overcome this problem is an exciting and practical solution. Nanoparticles can cause bacterial degradation by the electrostatic interaction with receptors and cell walls. Simultaneously, the incorporation of antibacterial materials such as zinc and graphene in nanoparticles can further enhance bacterial degradation. In the present study, zinc-doped hydroxyapatite/graphene was synthesized and characterized as a nanocomposite material possessing both antibacterial and bioactive properties for bone tissue engineering. After synthesizing the zinc-doped hydroxyapatite nanoparticles using a mechanochemical process, they were composited with reduced graphene oxide. The nanoparticles and nanocomposite samples were extensively investigated by transmission electron microscopy, X-ray diffraction, and Raman spectroscopy. Their antibacterial behaviors against Escherichia coli and Staphylococcus aureus were studied. The antibacterial properties of hydroxyapatite nanoparticles were found to be improved more than 2.7 and 3.4 times after zinc doping and further compositing with graphene, respectively. In vitro cell assessment was investigated by a cell viability test and alkaline phosphatase activity using mesenchymal stem cells, and the results showed that hydroxyapatite nanoparticles in the culture medium, in addition to non-toxicity, led to enhanced proliferation of bone marrow stem cells. Furthermore, zinc doping in combination with graphene significantly increased alkaline phosphatase activity and proliferation of mesenchymal stem cells. The antibacterial activity along with cell biocompatibility/bioactivity of zinc-doped hydroxyapatite/graphene nanocomposite are the highly desirable and suitable biological properties for bone tissue engineering successfully achieved in this work.

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Section: Cellular Behaviormentioning

confidence: 99%

“…Simultaneous Zn doping and rGO compositing improved the adsorption of molecules on the surface of nanoparticles. It has been reported that rGO facilitates the adsorption of ions and proteins at the surface of nanoparticles [29,30], leading to improvement in cell proliferation [44].…”

Section: Cellular Behaviormentioning

confidence: 99%

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

Self Cite

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“…While techniques such as directional freeze-casting have been previously employed for porogen-free and sintering-free approaches, they are limited in terms of pore size and mechanical strength, usually limited to microporosity and directionally anisotropic mechanical properties [15][16][17]. Additionally, additive manufacturing processes are amenable to the base calcium phosphate particle being structurally altered [18] or doped with additional bioactive ions, to improve osteo-inductivity [19] or mechanical strength [20] as well as to being combined with synergistic manufacturing processes such as freeze-casting [21] to avail benefits of both. Long term, additive manufacturing will have the ability to develop patient specific solutions to fit pre-imaged defect sites.…”

Section: Introductionmentioning

confidence: 99%

Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds

Montelongo

Chiou

Ong

et al. 2021

J Mater Sci: Mater Med

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Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds. Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability. Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic. The mechanical properties of the inks improved with increasing β-TCP content. The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved). The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods. The high interconnected porosity (45–60%) and fluid conductance (between 1.04 ×10−9 and 2.27 × 10−9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.

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Review of zirconia-based biomimetic scaffolds for bone tissue engineering

Weng

Hou

et al. 2021

J Mater Sci

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Effect of Nano-zirconia on Microstructure and Biological Behavior of Hydroxyapatite-Based Bone Scaffolds

Cited by 8 publications

References 32 publications

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds

Review of zirconia-based biomimetic scaffolds for bone tissue engineering

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