Effect of Nanoparticles on the Cell Life Cycle

Mahmoudi, Morteza; Azadmanesh, Kayhan; Shokrgozar, Mohammad Ali; Journeay, W. Shane; Laurent, Sophie

doi:10.1021/cr1003166

Cited by 313 publications

(245 citation statements)

References 244 publications

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“…Previous research has suggested that metal nanoparticles might potentially induce oxidative stress, leading to intracellular changes and alterations in the cell cycle. 30 In this study, we measured the change in percent proliferation index (percent synthesis phase of DNA [%S] + percent post-synthesis phase of DNA [%G 2 ]) in the cell cycle to indicate the effects of the TiO 2 nanoparticles on MSC.…”

Section: Cell Viability and Proliferation Assaymentioning

confidence: 99%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

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Background:The purpose of this study was to investigate the influences of nanoscale wear particles derived from titanium/titanium alloy-based implants on integration of bone. Here we report the potential impact of titanium oxide (TiO 2 ) nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells (MSC) from the cellular level to the molecular level in the Wistar rat. Methods: A series of TiO 2 nanoparticles (14 nm, 108 nm, and 196 nm) were synthesized and characterized by scanning electron microscopy and transmission electron microscopy, respectively. Results: The TiO 2 nanoparticles had negative effects on cell viability, proliferation, and the cell cycle of MSC in a dose-dependent and size-dependent manner. Confocal laser scanning microscopy was used to investigate the effects of particle internalization on adhesion, spreading, and morphology of MSC. The integrity of the cell membrane, cytoskeleton, and vinculin of MSC were negatively influenced by large TiO 2 nanoparticles. Conclusion: The Transwell migration assay and a wound healing model suggested that TiO 2 nanoparticles had a strong adverse impact on cell migration as particle size increased (P , 0.01). Furthermore, alkaline phosphatase, gene expression of osteocalcin (OC) and osteopontin (OPN), and mineralization measurements indicate that the size of the TiO 2 nanoparticles negatively affected osteogenic differentiation of MSC.

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Section: Cell Viability and Proliferation Assaymentioning

confidence: 99%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

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“…Nanoparticles (NPs), nanotechnological products of increasing importance due to their unique properties, have been used in several applications such as cosmetics, cleaning materials, electronic components, food additives, medicines, sports equipment and surface coatings (Mahmoudi et al 2011). However, the properties of NPs are different from those exhibited by the same material at macroscale and may cause undesired toxicity.…”

Section: Introductionmentioning

confidence: 99%

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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In recent years, the use of commercial nanoparticles in different industry and health fields has increased exponentially. However, the uncontrolled application of nanoparticles might present a potential risk to the environment and health. Toxicity of these nanoparticles is usually evaluated by a fast screening assay in zebrafish (Danio rerio). The use of this vertebrate animal model has grown due to its small size, great adaptability, high fertilization rate and fast external development of transparent embryos. In this review, we describe the toxicity of different micro-and nanoparticles (carbon nanotubes, dendrimers, emulsions, liposomes, metal nanoparticles, and solid lipid nanoparticles) associated to their biophysical properties using this model. The main biophysical properties studied are size, charge and surface potential due to their impact on the environment and health effects. The review also discusses the correlation of the effects of the different nanoparticles on zebrafish. Special focus is made on morphological abnormalities, altered development and abnormal behavior. The last part of the review debates changes that should be made in future directions in order to improve the use of the zebrafish model to assess nanotoxicity.

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“…Macropinocytosis evidently occurred between cells and particles larger than 100 nm. Although the average LNTPs particle size was only 62.1 nm, some particles were measured over 100 nm [18] , which might be the reason that macropinocytosis was involved in the uptake. Clathrin-dependent pinocytosis is used by all eukaryotic cells to internalize nutrients and degrade or recycle substances.…”

Section: Discussionmentioning

confidence: 98%

“…Clathrin-dependent pinocytosis is used by all eukaryotic cells to internalize nutrients and degrade or recycle substances. Thus, this process is well-known for its role in the selective uptake of molecules mediated by specific receptors [18] . Several studies suggested that BSA nanoparticles could be recognized by SPARC.…”

Section: Discussionmentioning

confidence: 99%

Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism

Zhang

Ruan

et al. 2014

Acta Pharmacol Sin

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Aim: Lapatinib is a dual inhibitor of EGFR and human epidermal growth factor receptor 2 (HER2), and used to treat advanced breast cancer. To overcome its poor water solubility, we constructed lapatinib-incorporated lipoprotein-like nanoparticles (LTNPs), and evaluated the particle characteristics and possible anti-breast cancer mechanisms. Methods: LTNPs (lapatinib bound to albumin as a core, and egg yolk lecithin forming a lipid corona) were prepared. The particle characteristics were investigated using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The uptake and subcellular localization of LTNPs, as well as the effects of LTNPs on cell cycle were examined in BT-474 human breast cancer cells in vitro. Mice bearing BT-474 subcutaneous xenograft were intravenously injected with coumarin-6 loaded LTNPs (30 mg/kg) to study the targeting mechanisms in vivo. Results: The LTNPs particles were generally spherical but flexible under TEM and AFM, and approximately 62.1 nm in size with a zeta potential of 22.80 mV. In BT-474 cells, uptake of LTNPs was mediated by endosomes through energy-dependent endocytosis involving clathrin-dependent pinocytosis and macropinocytosis, and they could effectively escape from endosomes to the cytoplasm. Treatment of BT-474 cells with LTNPs (20 μg/mL) induced a significant cell arrest at G 0 /G 1 phase compared with the same concentration of lapatinib suspension. In mice bearing BT-474 xenograft, intravenously injected LTNPs was found to target and accumulate in tumors, and colocalized with HER2 and SPRAC (secreted protein, acidic and rich in cysteine). Conclusion: LTNPs can be taken up into breast cancer cells through specific pathways in vitro, and targeted to breast cancer xenograft in vivo via enhanced permeability and retention effect and SPARC.

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Effect of Nanoparticles on the Cell Life Cycle

Cited by 313 publications

References 244 publications

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism

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