Effect of natural high temperature and flooding conditions on cry1ac gene expression in different transgenic Bt cotton (Gossypium hirsutum L.) cultivars

Ahmad, Shabbir; Majeed, Dilawar; Ali, Abid; Sufian, Muhammad; Aslam, Zubair; Manzoor, Mujahid; Ahmad, Jam Nazeer

doi:10.30848/pjb2021-1(38)

Cited by 4 publications

(3 citation statements)

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“…Furthermore, Cry1Ac endotoxin content varies across different cotton organs, with higher concentrations in the leaves and lower levels in reproductive organs like squares, owers, and bolls [14][15][16]. Adverse environmental conditions, including extreme temperatures, especially low temperatures, can signi cantly decrease the expression of insecticidal proteins [17][18][19][20][21][22][23]. Among these adverse environments, prolonged exposure to low temperatures has been shown to decrease the Cry1Ac endotoxin content in leaves throughout the growth period, with a more pronounced decrease during the bollsetting period [24].…”

Section: Introductionmentioning

confidence: 99%

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Chen,

Dong,

Dai

et al. 2024

Preprint

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Background Sudden temperature drops, resulting from extreme weather events, often occur during the boll-setting period of cotton in Xinjiang, China, causing decreased expression of Bacillus thuringiensis (Bt) insecticidal proteins in cotton bolls. The precise threshold temperatures and durations that lead to significant changes in Cry1Ac endotoxin levels under low temperatures remain unclear. To address this, we investigated the effects of different temperatures and stress durations on Cry1Ac endotoxin levels in cotton bolls. In 2020–2021, two Bt transgenic cotton cultivars, conventional Sikang1 and hybrid Sikang3, were selected as experimental materials. Various low temperatures (ranging from 16 to 20°C) with different durations (12h, 24h and 48h) were applied during the peak boll-setting period. Results As the temperature decreased, the Cry1Ac endotoxin content in the boll shell, fiber, and seed exhibited a declining trend. Moreover, the temperature causing a significant change in Cry1Ac endotoxin content increased with the prolonged duration of low-temperature stress. Among the components of cotton bolls, seeds were most affected by low-temperature stress, with the threshold temperature for a significant reduction in Cry1Ac endotoxin content ranging from 17°C to 19°C. Correlation analysis indicated that low temperatures led to a decrease in protein synthesis capacity and an increase in degradation ability, resulting in reduced Cry1Ac endotoxin content. Pathway analysis revealed that both amino acid and peptidase had significant negative effects on Cry1Ac endotoxin content. Conclusion In summary, when the daily average temperature was ≤ 19°C, implementing cultural practices to reduce free amino acid content and peptidase activity could serve as effective cold defense strategies for Bt cotton production.

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Section: Introductionmentioning

confidence: 99%

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Chen,

Dong,

Dai

et al. 2024

Preprint

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“…However, the use of pesticides led to the poisoning of food and the eradication of nontarget organisms. Additional hazards include the emergence of sucking pests environmental pollution and degradation consumer and farmer health concerns, and the adoption of resistance to synthetic pesticides [3]. Concerns over the widespread use of synthetic pesticides grew, prompting searches for more effective insect control measures.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness and Performance of BT Cotton's Insecticidal Properties under Various Abiotic Stresses: A Review

Shahid,

Aziz,

Fatima

et al. 2023

AJRAF

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Over the past two decades, biotechnology has yielded Bt cotton, a huge success in the field of agriculture. In a number of countries, commercial Bt crop production has proven successful. The global food scarcity crisis has been alleviated by transgenic Bt crops, which have improved agricultural productivity. The Bt cotton crop displays variable levels of insect resistance to several environmental abiotic circumstances, including high temperatures, salt in the soil, water logging and scarcity, inadequate nitrogen supply, and humidity. It has been demonstrated that these components lessen the transgenic crop's endotoxin protein level, which in turn reduces the insecticidal efficacy of the crop. In unfavourable climatic conditions, plants have altered nitrogen metabolism and pain in their physiological systems. This article has discussed some of the primary stresses that Bt cotton encounters as well as the underlying mechanisms that explain its variable insecticidal efficacy under both single and combination pressures. While several important elements are covered in this study, such as gene silencing, post transcriptional changes, and protein breakdown, the most important aspect of this field that still need investigation is nitrogen metabolism. A thorough meta-analysis table provides an overview of the several significant research that have been carried out to examine the impacts of stress. A comprehensive study technique is needed to clarify the metabolic and physiological changes that transpire in transgenic cotton crops under high stress. In order to increase the production and performance of the cotton crop worldwide, more research is needed to address the unpredictable insect resistance of Bt crops.

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“…Even though cotton is the main fber crop that plays a key role in the economic development of many countries in the world [10], the growth, yield, and fber quality of cotton are constrained by several abiotic and biotic factors [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Adaptability of Genetically Engineered Bt Cotton Varieties in Different Growing Regions of Ethiopia

Gudeta

Kedisso

Gurmessa

et al. 2023

Advances in Agriculture

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Cotton varieties that are high yielding and resistant to pests are required to improve production and productivity and to capitalize on the crop’s enormous potential and its critical role in Ethiopia’s expanding textile industry. Lack of improved cotton technology has forced farmers to recycle local varieties for ages which have become very susceptible to pests which are the major causes of very low productivity and quality of cotton in the country. Among major pests, bollworms (Helicoverpa armigera and Pectinophora gossypiella) account for 36–60% of yield losses. In the absence of genetically resistant or tolerant varieties, genetically engineered bollworm-resistant Bacillus thuringiensis (Bt) cotton has offered a great opportunity to reduce crop losses from bollworms. The objective of the study was to evaluate the efficacy of bollworm resistance and adaptability of Bt cotton varieties across cotton growing environments in Ethiopia and provide recommendations. Two Bt cotton hybrids (JKCH 1947 and JKCH 1050), one Bt OPV (Sudan), and three OPV conventional varieties (Weyito 07, Stam-59A, and Deltapine-90) were evaluated at seven different agro-ecologies using a randomized complete block design (RCBD) with three replications. Results showed significant differences among genotypes for yield and other traits. Hybrids JKCH 1947 and JKCH 1050 were the top high yielders under high and mild bollworm infestations, with mean seed cotton yield of 3.10 t·ha−1 each and lint yield of 1.20 and 1.19 t·ha−1, respectively, whereas the standard check Deltapine-90 (popular variety) recorded a mean seed cotton and lint yield of 2.3 t·ha−1 and 0.8 t·ha−1, respectively. Combined analysis showed that genotypes, environment, and the genotypes × environment interactions had a highly significant effect ( P < 0.05) on fiber quality. Weyito 07 and the two hybrids (JKCH 1947 and JKCH 1050) had upper half mean fiber lengths in the range of 27.78 to 32.11 mm. For fiber strength, genotypes Weyito 07, JKCH 1050, Stam-59A, and JKCH 1947 had 33.50 g/tex, 28.59 g/tex, 28.00 g/tex, and 27.75 g/tex, respectively. The fiber quality values of the hybrids were within acceptable limits, with staple lengths ranging from 27.78 to 28.44 mm and fiber strengths ranging from 27.75 to 28.59 g/tex. Results show potential adaptation of the hybrids under different cotton growing environments and their superior yield performance due also to added protection of yield losses from damage by bollworms. The contrast is bigger under high insect pressure conditions due to the genetically engineered Bt trait compared to the conventional varieties. The effective field resistance against bollworms in most locations shows that wider use of these hybrids can enhance cotton productivity and quality in Ethiopia.

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Effect of natural high temperature and flooding conditions on cry1ac gene expression in different transgenic Bt cotton (Gossypium hirsutum L.) cultivars

Cited by 4 publications

References 0 publications

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

The Effectiveness and Performance of BT Cotton's Insecticidal Properties under Various Abiotic Stresses: A Review

Adaptability of Genetically Engineered Bt Cotton Varieties in Different Growing Regions of Ethiopia

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