Effect of neonatal exposure to estrogenic compounds on development of the excurrent ducts of the rat testis through puberty to adulthood.

Abstract

Neonatal eposure to diethyistilbestrol (DES) can alter the structure of the testicular excurrent ducts in rats. We characterized these changes according to dose and time posttreatment and established whether potent estrogens (ethinyI estra , environmental estrogens (genistein, oct lbisphenol A, paraben) and tamoxifenainuce h c Ras were tered these compouinds neonatally and assesed at seerl time points during (day 10, or day 18 for some treatments) and after (cays 18, 25, 35, and 75) te treament period to detect any changes in testi weight, distension of the rete test and efferent ducts, epitelial cell height in the efferent ducts, and immunoexpression of the water channel aquaporin-1 (AQP-1).

Adverse effects of bisphenol A to spermiogenesis in mice and rats

“…There are those who say that BPA is not harmful to the male reproductive system (Ashby et al, 1999;Cagen et al, 1999a, b;Nagao et al, 1999Nagao et al, , 2002Atanassova et al, 2000). Others say that BPA affects the male reproductive system as evidenced by decreases in daily sperm production in mice (Vom Saal et al, 1998), increases in the prostate-weight in mice (Welshons et al, 1999), transient decreases in epithelial cell height of the rat efferent ducts at 3 weeks of age (Fisher et al, 1999), decreases in plasma free testosterone levels and the appearance of multinucleated giant cells in mouse seminiferous tubules (Takao et al, 1999), and the atrophy of rat seminiferous tubules (Takahashi and Oishi, 2001). Nakahashi et al (2001) showed that the decrease in daily sperm production was prevented by an administration of vitamin A along with BPA.…”

Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization

“…It is interesting to note that AQP-1 signals, like those of ER reported here, are intensely expressed in the efferent duct from the prenatal period through adulthood . Furthermore, in perinatal rats treated with diethylstilbesterol, AQP-1 immunoreactivity was abolished by postnatal day 18, accompanied by severe dilatation of efferent ducts, suggesting impaired function of fluid re-absorption (Fisher et al 1999). These  1.…”

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

“…3.2.4.1 Reproductive endpoints: Fisher et al (1999), supported by the European Centre for Ecotoxicology of Chemicals and Zeneca, examined the effect of neonatal bisphenol A exposure on excurrent ducts of the rat testis. On PND 2-12 (PND 1 5 day of birth), Wistar rat pups were s.c. injected with the corn oil vehicle or 37 mg/kg bw/day bisphenol A [purity not given].…”