Effect of Nitrate and Sulfate on Dechlorination by a Mixed Hydrogen-Fed Culture

Nelson, Denice; Hozalski, Raymond M.; Clapp, Lee W.; Semmens, Michael J.; Novak, Paige J.

doi:10.1080/10889860290777585

Cited by 32 publications

(29 citation statements)

References 16 publications

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“…A new method for direct hydrogen addition is the use of hollow-fiber membranes (Fang et al, 2002;Haugen et al, 2002;Muenzner et al, 2002;Nelson et al, 2002;Ma et al, 2003). This technology allows for the bubbleless dissolution of hydrogen, eliminating concerns over potential explosions (Gantzer, 1992).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Dechlorination by Membrane-Delivered Hydrogen: Small Field Demonstration

Edstrom

Semmens

Hozalski

et al. 2005

Environmental Engineering Science

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Biological reductive dechlorination can be used to remediate aquifers contaminated with chlorinated solvents. This process can be stimulated with the addition of hydrogen as an electron donor. A small field study was conducted in a trichloroethene (TCE)-contaminated aquifer at the Twin Cities Army Ammunition Plant site in Arden Hills, MN, to determine whether hollow-fiber membranes could be used to deliver hydrogen to the contaminated groundwater to stimulate bioremediation in situ. Nonporous siliconecoated membranes were placed in Geoprobe wells, where they were able to sustain hydrogen delivery over 20 months without a decline in gas transfer performance. High concentrations of hydrogen (168 Ϯ 13 M) were observed in the wells containing membrane modules. These concentrations were similar to that predicted using a clean water gas transfer gas correlation (255 M). High acetate concentrations (662 Ϯ 172 M) were also observed in the wells containing membrane modules during hydrogen addition. The addition of hydrogen via the hollow-fiber membranes stimulated vinyl chloride and ethene formation, likely as a result of the activity of acetotrophic dechlorinators.

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Section: Introductionmentioning

confidence: 99%

Stimulation of Dechlorination by Membrane-Delivered Hydrogen: Small Field Demonstration

Edstrom

Semmens

Hozalski

et al. 2005

Environmental Engineering Science

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“…The reported metabolic threshold for H 2 use for reductive dechlorination (<0.3 nmol L À1 ) and sulfate reduction (1-4 nmol L À1 ) are similar [9,11,12] and therefore have been reported to occur simultaneously. However, studies aimed at investigating the effect of sulfate on dechlorination reported either no inhibition (sulfate-reducers out-competed by dechlorinators at H 2 concentrations lower than 2.5 nmol L À1 ) [13,14], partial inhibition [15], or complete inhibition, even in the presence of excess H 2 [16].…”

Section: Introductionmentioning

confidence: 99%

Competition for H2 between sulfate reduction and dechlorination in butyrate-fed anaerobic cultures

Aulenta

Beccari

Majone

et al. 2008

Process Biochemistry

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“…In a recent study by Aulenta et al (2008), addition of sulfate (120 mg S/L) to a mixed dechlorinating culture resulted in a drastic decrease of the dechlorination rate of 1,1,2,2-tetrachloroethane compared to an identical culture devoid of sulfate. Previous reports on the effect of sulfate reduction on microbial dechlorination have shown either an absence of inhibition (Bagley and Gossett 1990;DeWeerd et al 1991;Hoelen and Reinhard 2004;Pavlostathis and Zhuang 1991), partial inhibition (Cabirol et al 1998), or complete inhibition (Nelson et al 2002). The difference among the above cited studies with respect to the effect of sulfate reduction on dechlorination may be due to the microbial diversity and relative population size of the different physiological groups resulting from difference in inocula origin and enrichment conditions (e.g., relative Fig.…”

Section: Pca Dechlorination and Sulfate Reductionmentioning

confidence: 95%

“…Inhibition of dehalogenation under sulfate-reducing conditions has been attributed to competition between sulfate and chlorinated compounds for electron donor (Alder et al 1993). Conflicting reports exist as to the effect of sulfate reduction on microbial dechlorination, from lack of inhibition (Bagley and Gossett 1990;DeWeerd et al 1991;Hoelen and Reinhard 2004;Pavlostathis and Zhuang 1991), to partial inhibition (Aulenta et al 2008;Cabirol et al 1998), or to complete inhibition (Nelson et al 2002). Considering the significant role molecular hydrogen (H 2 ) plays as a terminal electron donor in microbial reductive dechlorination reactions (Aulenta et al 2008;DiStefano et al 1992;Fennell et al 1997;Kassenga and Pardue 2006;Löffler et al 1999;Smatlak et al 1996;Yang and McCarty 1998), the outcome of competition among various microbial groups for H 2 utilization at relatively low H 2 concentrations may be explained by the fact that the H 2 threshold of dechlorinating bacteria (\0.3-2 nM) is significantly lower than that of hydrogenotrophic methanogens (5-95 nM), and similar to that of hydrogenotrophic sulfate reducers (1-4 nM) (Aulenta et al 2008).…”

Section: Introductionmentioning

confidence: 93%

Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture

Ismail

Pavlostathis

2009

Biodegradation

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The reductive dechlorination of pentachloroaniline (PCA) was investigated in the absence and presence of sulfate in batch assays using a PCA-dechlorinating mixed anaerobic culture with methanol as the external electron donor at neutral pH and 22 degrees C. PCA at an initial concentration of 7.8 microM was sequentially dechlorinated to dichlorinated anilines in the sulfate-free culture and the culture amended with 300 mg sulfate-S/L. At an initial concentration of 890 mg sulfate-S/L, a higher sulfate reduction rate was achieved, but PCA dechlorination was not observed until the sulfate concentration dropped to about 100 mg S/L. The transient inhibition of PCA is attributed to competition between sulfate reducing and dechlorinating species for electron donor, more likely for H(2) resulting from methanol fermentation. A long-term (118 days) PCA dechlorination assay with the sulfate-amended culture, which included five feeding cycles, resulted in accumulation of both sulfide (886 mg S/L) and acetate (1,900 mg COD/L). Under these conditions, the sulfate reducers were inhibited, while the rate and pathway of PCA dechlorination were not affected. The results of this study show that the rate of sulfate reduction rather than the sulfate concentration alone dictates the outcome of the competition between sulfate reducers and either dechlorinators or methanogens. The findings of the present study have significant implications relative to the fate and transport of PCA and its dechlorination products in sulfate-laden subsurface systems.

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Effect of Nitrate and Sulfate on Dechlorination by a Mixed Hydrogen-Fed Culture

Cited by 32 publications

References 16 publications

Stimulation of Dechlorination by Membrane-Delivered Hydrogen: Small Field Demonstration

Stimulation of Dechlorination by Membrane-Delivered Hydrogen: Small Field Demonstration

Competition for H2 between sulfate reduction and dechlorination in butyrate-fed anaerobic cultures

Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture

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