Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni

Akbari, Fatemeh; Arminian, Ali; Kahrizi, Danial; Fazeli, Arash

doi:10.14715/cmb/2017.63.2.17

Cited by 12 publications

(3 citation statements)

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“…Akbari et al, (2018) [84] revealed that different doses of nitrogen in growth media influence the growth and expression of UGT85C2, UGT74G1, and UGT76G1 genes in stevia in in vitro conditions. Therefore, evidence from our study and the other recent studies [46,85] suggests that the expression of SGs biosynthetic genes can be manipulated in in vitro conditions. Thus, the development of the commercial production of the high-value rebaudioside A can be achieved through growth media manipulation in tissue cultures in order to meet the increasing demands for natural sweeteners.…”

Section: Discussionsupporting

confidence: 60%

In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

et al. 2022

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A plant tissue culture protocol from stevia was optimized for the production of planting materials and the natural sweetener, rebaudioside A. The highest survivability (88.90% ± 5.55) of explants was achieved at 15 and 30 days after culture initiation (DACI) on Murashige and Skoog (MS) media by sterilization with 30% Clorox (5 min) and 10% Clorox (10 min), respectively. Supplementation of MS with 0.50 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.10 mg/L zeatin produced 50% callus at 15 DACI while 1.50 mg/L 2,4-D and 0.10 mg/L zeatin at 30 DACI increased callus production to 76.67%. The highest shoot proliferation per callus was achieved with 10.00 mg/L 6-benzyl amino purine (BAP) in MS at 15 DACI (5.80) and 30 DACI (12.33). The longest shoots of 4.31 cm and 6.04 cm at 15 and 30 DACI, respectively, were produced using BAP (10.00 mg/L) and 1.00 mg/L naphthalene acetic acid (NAA). MS media (0.50 strength) induced 2.86 and 6.20 roots per shoot and produced 3.25 cm and 7.82 cm long roots at 15 and 30 DACI, respectively. Stevia grown on 0.25 MS accumulated the highest concentration of rebaudioside A (6.53%), which correlated with the expression level of its biosynthetic gene uridine-diphosphate-dependent (UDP)-glycosyltransferase (UGT76G1).

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Section: Discussionsupporting

confidence: 60%

In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

et al. 2022

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“…In rapeseeds (Brassica napus) some lncRNAs have been detected to be differentially expressed in cadmium-exposed plants (42). Gene expression has been assayed against different treatments (47)(48)(49)(50). In A. thaliana the expression of 1832 identified lncRNAs were reported to be regulated by various abiotic stresses (51).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of 3Î²-HSD and mlncRNAs in response to abiotic stresses in Digitalis nervosa

Salimi¹,

Maroufi²,

Majdi³

2018

Cell Mol Biol (Noisy-le-grand)

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Digitalis nervosa is an important medicinal plant species belonging to the family of Scrophulariaceae that has the potential to be used for heart failure.3β-hydroxysteroid dehydrogenase (3β-HSD) is a key gene in the biosynthesis of cardenolides for making digitalis effective compounds, hence identification of this gene is important for genetic engineering purposes towards increasing the yield of cardiac glycosides. In addition, mRNA-like non-coding RNAs (mlncRNAs), a class of long non coding RNAs, play key roles in various biological processes and may affect cardenolides pathway in digitalis plants. In the present work, full sequence of 3β-HSD was isolated from Digitalis nervosa. Gene expression patterns of 3β-HSD along with three mlncRNAs including mlncRNA23, mlncRNA28 and mlncRNA30 were studied and the results indicated that they are differentially expressed in different tissues including roots, stems and leaves, with the highest expression levels in leaves. Moreover, the transcript levels of these genes affected by the cold and drought stresses. The results obtained from the present study is important in order to understand the potential role of mlncRNAs in digitalis plants, especially in response to abiotic stresses.

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“…High-performance liquid chromatography (HPLC) showed higher stevioside and rebaudioside A content in the leaves of Stevia grown on a PGR-free medium compared with the PGR(s)-containing media. Akbari et al [ 61 ] established a positive effect of the addition of inorganic nitrogen sources, i.e., NH 4 NO 3 (1650 mg/L) and KNO 3 (950 mg/L), to the MS medium on some morphological traits of S. rebaudiana Bertoni derived from axillary nodes as explants. Moreover, the presence of nitrogen sources increases both the shooting and rooting of the plant.…”

Section: Direct Organogenesismentioning

confidence: 99%

Effects of Different Elicitors on Micropropagation, Biomass and Secondary Metabolite Production of Stevia rebaudiana Bertoni—A Review

Miladinova-Georgieva

Geneva

Stancheva

et al. 2022

Plants

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Stevia rebaudiana Bertoni is a valuable plant whose products are increasingly used in medicine, pharmacy and the food industry. This necessitates the use of biotechnological approaches for its mass propagation. Establishing optimal conditions for in vitro cultivation is essential for obtaining high biomass and secondary metabolites production. A large number of articles considering the role of plant growth regulators and other additives in the culture medium in the growth and development of Stevia are available in the literature. However, there are no summarized data about the use of nanoparticles in Stevia tissue cultures. Therefore, this review also includes the research conducted so far on the effect of nanoparticles on Stevia micropropagation. Furthermore, the influence of different elicitors on secondary metabolite production and antioxidant activity of in vitro-cultivated Stevia plants have been discussed. By referring to the collected literature, we concluded that biotechnological approaches applied to S. rebaudiana cultivation might improve the agronomic traits of plants and steviol glycosides production.

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Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni

Cited by 12 publications

References 0 publications

In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

Differential expression of 3Î²-HSD and mlncRNAs in response to abiotic stresses in Digitalis nervosa

Effects of Different Elicitors on Micropropagation, Biomass and Secondary Metabolite Production of Stevia rebaudiana Bertoni—A Review

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