In this study, food staples comprising of Zea mays (maize), Sorghum bicolor (guinea corn), Glycine max (soybean), Arachis hypogea (groundnut) and Procambarus clarkii (crayfish) were traditionally processed and formulated into maize-guinea corn based weaning diets at 16% protein level. A replica of each diet was formulated with inclusion of vitamin C (50 mg/100 g diet). Fifty four 3-week old weanling albino rats (Rattus norvegicus) weighing between 36-39 g were placed into 13 groups of 4 rats per group and administered different diet groups ranging from maize- guinea corn base diet alone, through staples-inclusive diet (singly and in combination) and their vitamin c fortified replica diets, and standard commercial infant formula (Cerelac®) diet. Animals were sacrificed on the 28th day from onset of feeding and evaluated for biochemical, hematological and biological parameters; and sensory evaluation also conducted on the diets. Results showed significant increases in serum concentrations of protein, urea, creatinine; in the levels of red blood cell (RBC), hemoglobin (Hb) and packed cell volume (PCV); in total body weight gain and organ weights and insignificant changes in white blood cell (WBC) and platelet counts in the staples-inclusive diets compared to base diet and also in the vitamin C- fortified diets compared to non-fortified diets. Incorporation of food staples enhanced the protein quality of the diets while fortification with vitamin C increased only the biological value. On the acceptability rating, only the base gruel-crayfish mix showed significantly higher taste and overall acceptability. Therefore, the inclusion of the food blends comprising of soybean, groundnut and crayfish improved the nutritive content, hematopoietic potential, protein quality, taste and overall acceptability of the maize-guinea corn based weaning diet. These were further improved by fortification of the diets with vitamin C, while crayfish played a prominent role in taste and overall acceptance of the formulated diets. Fortification also significantly bridges the gap between the formulated weaning diets and Cerelac®, a standard commercial weaning formula in Nigeria.