Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation

Ádám-Vizi, Vera; Ördögh, Mária; Horváth, István T.; Somogyi, J.; Vizi, E.S.

doi:10.1007/bf01256639

Cited by 18 publications

(2 citation statements)

References 23 publications

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“…It is well known from several studies that noradrenaline is capable of increasing Na+ ,K+-ATPase activity in different parts of the brain (Schaefer et al, 1972;Yoshimura, 1973;Godfraind et al, 1974;Lee and Phillis, 1977;Adam-Vizi et al, 1979). Recently we have shown that vanadate can inhibit Na+,K+-ATPase activity without affecting Mgz+-ATPase activity in a synaptosomal preparation from rat brain cortex (Adam-Vizi et al, 1980). This inhibition could be partly reversed by noradrenaline, which is in line with an earlier finding of .…”

Section: M)supporting

confidence: 89%

“…It was suggested that the stimulatory effect of noradrenaline on enzyme activity might be related to its ability to reverse the inhibition of the enzyme caused by vanadate Adfim-Vizi et al, 1980). The suggestion seemed to be very feasible, particularly as when vanadium-free ATP was used as substrate noradrenaline was no longer able to increase enzyme activity in synaptosomes unless they had been previously exposed to vanadate (Adam-Vizi et al, 1980).…”

Section: M)mentioning

confidence: 99%

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Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

Ádám-Vizi

Váradi

Simon

1981

Journal of Neurochemistry

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The effect of ascorbic acid and noradrenaline on the inhibition of synaptosomal membrane ATPase by vanadate has been studied. Ascorbic acid (2 x 10(-3) M) and noradrenaline (10(-4) M) partly reversed the inhibition by vanadate (10(-6) M); however, when both were administered together the inhibition was completely eliminated. Using electron spin resonance (ESR) spectroscopy, we detected that ascorbic acid (10(-3) M) caused a 42% of reduction of vanadate (10(-4) M). Noradrenaline (10(-4) M) alone also reduced vanadate (10(-4) M) partially. When ascorbic acid and noradrenaline were present together all the vanadate was reduced to vanadyl. The concentration of ascorbic acid present in the brain under physiological conditions is identical to that found effective in our experiments. We suggest that ascorbic acid may protect the ATPase, at least in part, from inhibition by vanadate as a consequence of reducing vanadate to vanadyl. In those tissues where noradrenaline is also present a complete reduction of endogenous vanadium can be presumed.

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Section: M)supporting

confidence: 89%

Section: M)mentioning

confidence: 99%

Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

Ádám-Vizi

Váradi

Simon

1981

Journal of Neurochemistry

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In vivo electrical stimulation alters sensitivity of the brain (Na⁺ + K⁺)ATPase toward inhibition by vanadate

Krivánek

1981

J. Neurobiol.

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It has recently been shown that electrical stimulation of the brain cortex in vivo blocks invasion of cortical spreading depression (SD) into the stimulated area. The effect has been interpreted as a result of activating a K+ pumping mechanism that prevents the accumulation of this ion in the extracellular space to the high levels required for SD propagation. In the present experiments (Na+ + K+)ATPase activity was determined in the electrically stimulated region of the rat brain cortex. When ATP preparations containing vanadate were used as substrate, elevation of K concentration in the assay medium from 2 to 20 mM inhibited enzyme activity in homogenates from the normal cortex but not that from homogenates of the electrically stimulated cortical region. With vanadate-free ATP (Boehringer) as a substrate, slight stimulation by 20 mM K+ has been observed in both cases. Vanadate (0.25 microM) added to the assay medium containing Boehringer ATP and 20 mM K+ inhibited ATPase activity from the normal cortex but not that from the stimulated cortical area. Electrical stimulation may activate (Na+ + K+)ATPase at least partly by diminution of its susceptibility toward the inhibitory action of vanadate.

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Na+,K+-ATPase activity in cultured C6 glioma cells

1989

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Rat C6 glioma cells were cultured for 4 days in MEM medium supplemented with 10% bovine serum and Na+, K+-ATPase activity was determined in homogenates of harvested cells. Approximately 50% of enzyme activity was attained at 1.5 mM K+ and the maximum (2.76 +/- 0.13 mumol Pi/h/mg protein) at 5 mM K+. The specific activity of Na+, K+-ATPase was not influenced by freezing the homogenates or cell suspensions before the enzyme assay. Ten minutes' exposure of glioma cells to 10(-4) or 10(-5) M noradrenaline (NA) remained without any effect on NA+, K+-ATPase activity. Neither did the presence of NA in the incubation medium, during the enzyme assay, influence the enzyme activity. The nonresponsiveness of Na+, K+-ATPase of C6 glioma cells to NA is consistent with the assumption that alpha (+) form of the enzyme may be preferentially sensitive to noradrenaline. Na+, K+-ATPase was inhibited in a dose-dependent manner by vanadate and 50% inhibition was achieved at 2 x 10(-7) M concentration. In spite of the fact that Na+, K+-ATPase of glioma cells was not responsive to NA, the latter could at least partially reverse vanadate-induced inhibition of the enzyme. Although the present results concern transformed glial cells, they suggest the possibility that inhibition of glial Na+, K+-ATPase may contribute to the previously reported inhibition by vanadate of Na+, K+-ATPase of the whole brain tissue.

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Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation

Cited by 18 publications

References 23 publications

Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

In vivo electrical stimulation alters sensitivity of the brain (Na⁺ + K⁺)ATPase toward inhibition by vanadate

Na+,K+-ATPase activity in cultured C6 glioma cells

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Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation

Cited by 18 publications

References 23 publications

Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

Reduction of Vanadate by Ascorbic Acid and Noradrenaline in Synaptosomes

In vivo electrical stimulation alters sensitivity of the brain (Na+ + K+)ATPase toward inhibition by vanadate

Na+,K+-ATPase activity in cultured C6 glioma cells

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In vivo electrical stimulation alters sensitivity of the brain (Na⁺ + K⁺)ATPase toward inhibition by vanadate