Effect of novel bioresorbable scaffold composed of poly-l-lactic acid and amorphous calcium phosphate nanoparticles on inflammation and calcification of surrounding tissues after implantation

Feng, Gaoke; Qin, Chaoshi; Yi, Xin; Xia, Jinggang; Chen, Jingjing; Chen, Xiyu; Chen, Tao; Jiang, Xuejun

doi:10.1007/s10856-018-6125-6

Cited by 13 publications

(8 citation statements)

References 19 publications

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“…Notably, we observed reduced inflammation of tissues in mice implanted with PLA-RGD coupons (Figure b, E,F). PLA implants have been reported to cause inflammation in surrounding tissues due to the acidity of its degradation by products. − Our observation of reduced inflammation in mice following the implantation of PLA-RGD coupons makes the PLA-RGD brush copolymer a promising bioadditive for use in PLA-based implants to manage inflammatory responses arising from PLA biodegradation. Inflammatory response studies for long-term implantation of up to 6 months would be conducted in future studies to study the effect of biodegradation and inflammatory control of the material in vivo .…”

Section: Resultsmentioning

confidence: 90%

Bioactive PCL-Peptide and PLA-Peptide Brush Copolymers for Bone Tissue Engineering

Teo

Park

Guo

et al. 2022

ACS Appl. Bio Mater.

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We report the modular synthesis of bioactive brush-type polycaprolactone-peptide and polylactide-peptide copolymers for applications in bone tissue engineering. The brush copolymers containing pendant side chains of polycaprolactone (PCL) or polylactide (PLA) and PEGylated peptides, including linear Arg-Gly-Asp and collagen-like peptide (Gly-Pro-Hyp)3, were synthesized by ring-opening metathesis polymerization with high conversions and low dispersities (<1.5). These PCL-peptide and PLA-peptide copolymers exhibited good thermal stability for material processing using melt-extrusion-based methods. The copolymers were blended with commercial PCL or PLA, extruded into filaments, and 3D printed using fused filament fabrication methods. These bioactive PCL and PLA materials promoted osteogenic differentiation in vitro and showed good biocompatibility in in vivo murine model study. The promising results presented herein will serve as a useful guide for the design and functionalization of PCL or PLA materials for use in bone tissue engineering.

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Section: Resultsmentioning

confidence: 90%

Bioactive PCL-Peptide and PLA-Peptide Brush Copolymers for Bone Tissue Engineering

Teo

Park

Guo

et al. 2022

ACS Appl. Bio Mater.

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“…24 Several studies have reported that PLLA generated lactic acid during the processes of degradation; the accumulated degradation metabolites deprived the cell growth and provoked inflammation in the surrounding tissues. 55,56 This may explain why the cell viability of PUA2 and PUA3 did not increase from 10 to 14 days. On the other hand, the shear thinning behavior of materials is likely to result in cell injury during the printing process because of the shear stresses inflicted on cells in viscous fluids.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Characterization of Dual Stimuli-Sensitive Biodegradable Polyurethane Soft Hydrogels for 3D Cell-Laden Bioprinting

Hsiao

Hsu

2018

ACS Appl. Mater. Interfaces

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Three-dimensional bioprinting serves as an attractive platform to fabricate customized tissue-engineered substitutes from biomaterials and cells for the repair or replacement of injured tissues and organs. A common challenge for 3D bioprinting materials is that the structures printed from the biodegradable polymer hydrogels tend to collapse because of the poor mechanical stability. In this study, dual stimuli-responsive biodegradable polyurethane (PU) dispersions (PUA2 and PUA3) were synthesized from an eco-friendly waterborne process. Acrylate group was introduced in the PU chain end to serve as a photosensitive moiety for UV-induced cross-linking and improvement of the printability, while mixed oligodiols in the soft segment remained to be the thermosensitive moiety. The photo/thermal-induced morphological changes of PU nanoparticles were verified by dynamic light scattering, small-angle X-ray scattering, and rheological measurement of the dispersions. It was observed that these PU nanoparticles became more rod-like in shape after UV treatment and formed compact packing structures upon further heating. With the thermosensitive properties, these UV-cured PU dispersions underwent rapid thermal gelation with gel moduli in the range 0.5-2 kPa near body temperature. The rheological properties of the PU hydrogels including dynamic viscoelasticity, creep recovery, and shear thinning behavior at 37 °C were favorable for processing by microextrusion-based 3D printing and could be easily mixed with cells before printing to produce cell-laden constructs. The dual-responsive hydrogel constructs demonstrated higher resolution and shape fidelity as well as better cell viability and proliferation than the thermoresponsive control. Moreover, the softer hydrogel (PUA3) with a low modulus (<1 kPa) could offer neural stem cells a tofu-like, stable, and inductive 3D microenvironment to proliferate and differentiate. We expect that the photo/thermoresponsive biodegradable polyurethane ink may offer unique rheological properties to contribute toward the custom-made bioprinting of soft tissues.

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“…Besides, the expression level of IL-6 increased with time in the PLLA group. The above results suggested that the inflammatory reaction of the vascular tissue around the scaffold was gradually aggravated with the prolongation of the implantation time in the PLLA scaffold [17].…”

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confidence: 83%

Evaluation of Long-Term Inflammatory Responses after Implantation of a Novel Fully Bioabsorbable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles

Gao

Nguyen

et al. 2018

Journal of Nanomaterials

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Objectives. Our previous studies have confirmed the superior biocompatibility of the poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffolds compared to PLLA scaffolds at 1-month. In the present study, the long-term inflammatory responses of PLLA/ACP scaffolds in a porcine coronary model have been explored. Methods. The 24 PLLA scaffolds and 24 PLLA/ACP scaffolds were implanted into the coronary arteries of 24 miniature pigs. Serum levels of ALT, AST, and CRP were measured before operation, as well as 1-month, 6-months, 12-months, and 24-months. The vascular segments were taken for pathomorphological observation. HE staining was used for the inflammatory score and fibrosis score. Immunohistochemical staining detected positive expression indexes of MMP-9 and NF-κB. The expression of inflammation-related proteins of IL-1 and IL-6 was detected by Western Blot in surrounding tissues of scaffolds. Results. There was no significant difference between the two groups in ALT, AST, and UR at different time points (P < 0 05). The inflammation score in the PLLA/ACP group was lower than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 05), and the fibrosis score was reduced in the PLLA/ACP group than that in the PLLA group at 12-months and 24-months (P < 0 05). The expression of MMP-9 and NF-κB in the PLLA/ACP group was significantly less than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 05). The protein expression of IL-1 in the PLLA/ACP group was decreased than that in the PLLA group at 12-months and 24-months (P < 0 05). Furthermore, the protein expression of IL-1 was significantly lower than that in the PLLA group at 6-months, 12-months, and 24-months (P < 0 01). Conclusions. The supplement of ACP nanoparticles can effectively reduce the long-term inflammatory reaction caused by PLLA and has good safety and biocompatibility. The novel bioabsorbable PLLA/ACP scaffold provides reliable guidance for the development and clinical application of bioabsorbable scaffolds in the future.

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Effect of novel bioresorbable scaffold composed of poly-l-lactic acid and amorphous calcium phosphate nanoparticles on inflammation and calcification of surrounding tissues after implantation

Cited by 13 publications

References 19 publications

Bioactive PCL-Peptide and PLA-Peptide Brush Copolymers for Bone Tissue Engineering

Bioactive PCL-Peptide and PLA-Peptide Brush Copolymers for Bone Tissue Engineering

Synthesis and Characterization of Dual Stimuli-Sensitive Biodegradable Polyurethane Soft Hydrogels for 3D Cell-Laden Bioprinting

Evaluation of Long-Term Inflammatory Responses after Implantation of a Novel Fully Bioabsorbable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles

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