Effect of Nutrient Media and Phytohormones on<i> In Vitro</i> Establishment of <i>Bambusa balcooa.</i> Roxb.

Abstract

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company’s green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the

Penggunaan Pupuk Conway Pada Media Kultur Terhadap Pertumbuhan Bibit Rumput Laut Eucheuma cottonii

“…Kerjasama antara Kementerian Kelautan dan Perikanan Indonesia dan SEAMEO BIOTROP tahun 2011, menghasilkan rumput laut kultur jaringan yang pertama kali diproduksi di Indonesia untuk meningkatkan kualitas bibit rumput laut E. cottonii atau K. alvarezii melalui teknik kultur jaringan (Basiroh et al, 2016). Kultur jaringan adalah kegiatan yang berkaitan dengan perbanyakan sel, organ, dan jaringan bagian lainnya pada tumbuhan secara aseptik dalam kondisi in vitro (Khan et al, 2014). Beberapa faktor yang mempengaruhi pertumbuhan dan perkembangan sel untuk kultur jaringan yaitu jenis media, zat pertumbuhan, sumber eksplan, dan lingkungan kultur jaringan (Yusnita, 2004).…”