Effect of Nutrient Media Composition on Sugar-Free Growth and Chlorophyll Fluorescence of Pinus Radiata Shoots in Vitro

Aitken-Christie, J.; Davies, Helen; Holland, L.; Kubota, Chieri; Fujiwara, Kazuhiro

doi:10.17660/actahortic.1992.319.14

Cited by 21 publications

(3 citation statements)

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“…Plantlets from the more photoautotrophicmedium (0.5% sucrose) adapted better to intermediate PPFD, and even at hiigh PPFD tended to show less severe photoinhibition than those from the more heterotrophic medium (3.0% sucrose). In this regard, recent reports on micropropagated Radiata pine (Aitken-Christie et al, 1992) and strawberry plantlets (see Hdider & Desjardins, 1994) show higher Fo/Fm ratios for shoots cultured with 0% or 1% sucrose media as compared to those with 3% or 5%. In summary our results support the hypothesis that the plantlets most affected by highest PPFD during culture in vitro would be those with lowest development of photosynthetic characteristics, either due to culture conditions or because they are at an earlier stage of micropropagation.…”

Section: Discussionmentioning

confidence: 87%

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Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation

Serret

Trillas

Matas

et al. 1996

Plant Cell Tiss Organ Cult

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This paper reports on the fast fluorescence responses of Gardeniajasminoides Ellis plantlets, at two successive stages (shoot multiplication and root induction) of culture in vitro. We test whether plantlets in vitro suffer photoinhibition during culture and whether the degree of photoautotrophy of these mixotrophic plantlets has any effect on the extent of photoinhibitory impairment. In this regard the effects of different sucrose levels in the medium and PPFD during growth on the development of photoautotrophy and the extent of photoinhibition were evaluated. Plantlets were grown under low, intermediate, and high (50, 100, and 300 #mol m -2 s -1) PPFD, and at 3 different sucrose concentrations (0.5, 1.5, and 3.0%, w/v) in the medium, during shoot multiplication. During root induction the same growth conditions were assayed except for the high PPFD. The development of photoautotrophy was assessed via the difference between the stable carbon isotope composition of sucrose used as heterotrophic carbon source and that of leaflets grown in vitro. Plantlets from root induction showed more developed photoautotrophy than those from shoot multiplication. For both stages the low-sucrose medium stimulated the photoautotrophy of plantlets in vitro. In addition, intermediate PPFD induced photoautotrophy during shoot multiplication. For plantlets of both culture stages at the lowest PPFD no photoinhibition occurred irrespective of the sucrose concentration in media. However, during the shoot multiplication stage chlorophyll fluorescence measurements showed a decrease in F J F m and in tl/2 as growing PPFD increased, indicating photoinhibitory damage. The decline of Fv/Fm was caused mostly by an increase in Fo, indicating the inactivation of PSII reaction centers. However plantlets growing under low sucrose showed reduced susceptibility to photoinhibition. During root induction, only plantlets cultured with high sucrose showed a decrease in F~/F,,~ as PPFD increased, although tj/2 remained unchanged. In this case, the decline of FolFm was mostly due to a decrease in Fro, which indicates increased photoprotection rather than occurrence of photodamage. Therefore, growth in low-sucrose media had a protective effect on the resistance of PSII to light stress. In addition, plantlets were more resistant to photoinhibition during root induction than during shoot multiplication. Results suggest that increased photoautotrophy of plantlets reduces susceptibility to photoinhibition during gardenia culture in vitro.Abbreviations: AP -apparent photosynthesis; Chl -total chlorophyll content; Chl a/b -chlorophyll a-to-b ratio; Chl/Car -total chlorophyll-to-carotenoids ratio; 613C -ratio of 13C/12C relative to PeeDee belemnite standard; F m -maximum chlorophyll fluorescence; Fo -fluorescence emission when all reaction centres are open and the photochemical quenching is minimal; Fo -variable chlorophyll fluorescence (Fro -Fo); FvlFm -the ratio of variable to maximum chlorophyll fluorescence, indicator photochemical efficiency of PSII; MS mediu...

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Section: Discussionmentioning

confidence: 87%

“…However, the absorption of excessive light energy by leaves has been recognized as a source of damage not only in the field (see Boyer et al, 1987) but also in vitro (Aitken-Christie et al, 1992). In addition, the combination of high light and high temperatures (e.g.…”

Section: Introductionmentioning

confidence: 99%

Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation

Serret

Trillas

Matas

et al. 1996

Plant Cell Tiss Organ Cult

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“…In fact, there were several reports on the effects of culture conditions on Fv/Fm. Nutrient composition of the medium affected Fv/Fm of in vitro cultured Pinus radiata (Aitken-Christie et al 1992). Differences in Fv/Fm due to the difference in culture conditions have also been reported in potato micropropagation (Ibaraki and Matsumura 2005).…”

Section: Introductionmentioning

confidence: 78%

Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity

Ibaraki

Gupta

2010

In Vitro Cell.Dev.Biol.-Plant

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The photochemical reflectance index (PRI) of micropropagated potato leaves was estimated nondestructively from outside the culture vessel using a PRI imaging system developed by the present group. The PRI was determined under low light intensity conditions after dark treatment and compared with the chlorophyll fluorescence parameter Fv/Fm, which denotes photosystem II maximum quantum yield. Short-term high-light treatment decreased Fv/Fm of the plantlets. Culture conditions such as temperature and sucrose concentration also affected Fv/Fm. A linear relationship between the PRI and Fv/Fm was observed in both cases of high-light treatment and different culture conditions, suggesting the potential of the PRI to be used as a substitute for Fv/Fm. PRI estimated from reflection images under low light intensity conditions may be used for rapid and noninvasive evaluation of photosynthetic properties of micropropagated plantlets in a similar manner to Fv/Fm.

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Plant Species Successfully Micropropagated Photoautotrophically

Kubota

Afreen²,

Zobayed³

Photoautotrophic (Sugar-Free Medium) Micropropagation as a New Micropropagation and Transplant Production System

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Effect of Nutrient Media Composition on Sugar-Free Growth and Chlorophyll Fluorescence of Pinus Radiata Shoots in Vitro

Cited by 21 publications

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Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation

Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation

Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity

Plant Species Successfully Micropropagated Photoautotrophically

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