Effect of oestrous cycle and early pregnancy on uterine production and expression of immune regulatory factors in gilts

Chabot, V.; Lambert, R.; Laforest, Jean-Paul; St‐Jacques, Sylvie; Matte, J. J.; Guay, Frédéric; Palin, Marie‐France; Lessard, Marie-Hélène

doi:10.1016/j.anireprosci.2003.08.008

Cited by 10 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is unlike what is found in bovine, where GMCSF is up‐regulated in response to conceptus‐derived PGE2 and interferon tau (IFNT) during pregnancy in immune and nonimmune cells, but is reduced at the site of implantation (Emond et al, ). These observations are further complicated by an earlier report that GMCSF was undetectable between Days 10 and 15 in either cycling or pregnant animals (Chabot et al, ). Thus, the response of GMCSF to PGE2 and its actions are quite complex, with both inhibitory and stimulatory effects (Li and Fox, ).…”

Section: Discussionmentioning

confidence: 82%

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Jalali

Kitewska

Wasielak

et al. 2014

Molecular Reproduction Devel

View full text Add to dashboard Cite

Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune changes in leukocyte populations were measured by flow cytometry and mRNAs for various cytokines by quantitative reverse-transcriptase PCR in porcine endometrium collected on Days 6 and 13 from cycling and pregnant animals or from animals given seminal plasma infusions. Seminal plasma infusion induced long-term modulatory effects, resulting in significantly more endometrial FoxP3-positive T-regulatory and T-helper cells 6 days after infusion as compared to cycling and pregnant animals. The number of T-cytotoxic and T-null cells did not change between the studied groups. The early molecular effects of seminal plasma were not observed at 13-days post-infusion, although animals on Day 13 of pregnancy did show significantly more T-cells (of any type investigated). Seminal plasma also showed a delayed effect on cytokine expression, specifically exhibiting a significant increase in interleukin 10 (IL10) and a decrease in granulocyte macrophage colony-stimulating factor (GMCSF) gene expression on Day 13 as compared to Day 6 of cycling or pregnant gilts. The results indicate a delayed regulatory effect of seminal plasma on immune responses in the porcine uterus, which are similar to immune changes generated by implanting conceptuses.

show abstract

Section: Discussionmentioning

confidence: 82%

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Jalali

Kitewska

Wasielak

et al. 2014

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…In the current study, the lower mRNA abundance of PPARd found in day 15 pregnant sows when compared with day 15 cyclic sows suggests a limited role of PPARd during the periattachment period. Previous studies have reported higher PGE 2 in uterine fluid of day 15 pregnant gilts when compared to the day 15 cyclic gilts (Chabot et al 2004) and higher PGF 2a and PGE 2 levels in uterine flushings of pregnant relative to non-pregnant gilts (Geisert et al 1982). Thus, the elevated levels of prostaglandins (Chabot et al 2004) and the lower mRNA abundance of PPARd (current study) in day 15 pregnant sows suggest that PPARd may be of modest significance in the mediation of action of endometrial and conceptus-derived prostaglandins during the attachment period.…”

Section: Discussionmentioning

confidence: 99%

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Lord

Murphy²,

Desmarais³

et al. 2006

Reproduction

View full text Add to dashboard Cite

Recent evidence points to a role for peroxisome proliferator-activated receptors (PPARs) d and g in embryo implantation and survival. In this study, we report the porcine PPARd complete coding sequence and mRNA abundance of PPARd, PPARg1 and g2, angiopoietin-like protein 4 (ANGPTL4) and adipocyte determination and differentiation-dependent factor 1 (ADD1) genes in the pregnant sow endometrium. Real-time PCR analysis was used to study the effect of parity (Yorkshire-Landrace multiparous (YL) and nulliparous (YLn)), site of endometrial tissue sampling (between and at embryo attachment sites) in crossbred Duroc!Yorkshire-Landrace (DYL) sows and stages of pregnancy (non-pregnant, day 15 and day 25 after mating) in Meishan-Landrace (ML) on mRNA levels. Parity effects were observed for PPARd, ANGPTL4, and ADD1, with higher mRNA levels in YL than YLn sows. In DYL sows, lower mRNA levels were present at attachment sites compared to between attachment sites for PPARd, PPARg1, and ANGPTL4. Finally, day 15 pregnant ML sows had lower PPARd mRNA levels compared to day 15 cycling ML sows. A significant increase of PPARg1 mRNA levels was found on day 25 pregnant ML and DYL sows relative to day 15 ML or DYL pregnant sows. PPARd and g immunostaining was detected in endometrial tissue of day 15 cycling sows, day 15 and 25 pregnant sows and epithelial cells of day 25 embryos. Collectively, our results suggest a role for PPARd, PPARg1, and ANGPTL4, but not PPARg2, during the peri-implantation period in pregnant sows. Reproduction (2006) 131 929-942

show abstract

“…Many authors have used the porcine model for such experiments (Kennedy et al 1994, Chabot et al 2004. Therefore, our results supply some new information on the understanding of mechanisms of influence of TGF and EGF systems on endometrial tissue growth, as well as tissue differentiation in bitches during various estrus cycle stages.…”

Section: Discussionmentioning

confidence: 96%

Differential expression of epidermal growth factor and transforming growth factor beta isoforms in dog endometrium during different periods of the estrus cycle

Bukowska

Kempisty²,

Jackowska³

et al. 2011

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Both epidermal growth factor (EGF) and transforming growth factor (TGF) play an important physiological role in the processes of proliferation and differentiation of several different cell types. However, the expression profiles of these factors in domestic bitches endometrium are still poorly recognized. The aim of the present study was to identify and analyze the differential expression of these factors in various stages of the estrus cycle.Endometrial tissue from proestrus (n=17), estrus (n=10), day 10 diestrus (n=15), day 35 diestrus (n=18) and anestrus (n=25) was collected soon after ovariohysterectomy. Total RNA was isolated from the endometrium by means of Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. Quantitative analysis of EGF, TGFβ1, TGFβ2, and TGFβ3 cDNA was performed by real-time quantitative polymerase chain reaction (RT-PCR).EGF expression in canine endometrium was increased in the estrus stage as compared to proestrus (P<0.05), day 10 diestrus (P<0.05), day 35 diestrus (P<0.01) and anestrus (P<0.001). We also found the differences in EGF expression between day 10 and day 35 of estrus as well as between day 35 of estrus with anestrus (P<0.05, P<0.01, respectively). The TGFf1 transcript contents were also higher in estrus as compared to other stages (P<0.01). The TGFβ2 and TGFβ3 in the estrus stage was increased compared to proestrus, day 10 diestrus, day 35 diestrus and anestrus (P<0.05).We proved that expression of EGF and TGFβ transcript isoforms is related to the phase of estrus in bitches and therefore may be regulated by specific hormone concentrations during these periods. Our results confirm the hypothesis that these growth factors play a role in the regulation of biochemical changes in the endometrial tissues during the estrus cycle.

show abstract

Effect of oestrous cycle and early pregnancy on uterine production and expression of immune regulatory factors in gilts

Cited by 10 publications

References 38 publications

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Differential expression of epidermal growth factor and transforming growth factor beta isoforms in dog endometrium during different periods of the estrus cycle

Contact Info

Product

Resources

About