Effect of olive mill wastewater on growth and bulb production of tulip plants infected by bulb diseases

Abstract

The effect of olive mill wastewater (OMW) on growth of tulip plants infected by common diseases as well as on their new bulbs production is analyzed in this work. Filtered and sterilized OMW was tested as growth inhibitor of Botrytis tulipae, Fusarium oxysporum, Aspergillus niger and Penicillium spp. mycelium. The effect of filtered OMW on uninfected tulip bulbs was also tested as well as on the growth of bulbs infected with the fungus B. tulipae and A. niger in vivo. The mycelium length, severity of scab-like lesions, plant height (PH), fresh mass (FM) and dry mass (DM) of plants and production of new bulbs were recorded. Only the filtered OMW inhibited the in vitro mycelium growth of all tested fungi. However filtered OMW caused infections when it sprayed on uninfected bulbs, malformations on 30% of the plants grown from these bulbs and decrease PH, FM and DM as well as new bulbs production at 75%, 72.4%, 79.1% and 50% respectively. The treatment of B. tulipae infected bulbs with filtered OMW reduced further the PH, FM, DM and the production of new bulbs in 92.1%, 81.4%, 78.7% and 97% respectively. In contrast the treatment of infected bulbs by B. tulipae + A. niger with filtered OMW did not affect PH, FM and the number of new bulbs produced and significantly improved plants DM and the mass of new bulbs.

Potential Synergistic Action of Liquid Olive Fruit Polyphenol Extract with Aqueous Extracts of Solid Wastes of Pomegranate or/and Orange Juice Industry as Organic Phyto-protective Agents against Important Plant Pathogens - Part 1 (in vitro Studies)

“…Furthermore, studies on the use of OMWW has been demonstrated that it can act as phytoprotective compound on fruits and vegetables during the growing season and after harvest during storage offering a promising solution for preventing losses of fruits and vegetables from post-harvest attacks such as those from the fungus B. cinerea [69,73]. Similar results have been reported by other studies [65,[91][92][93] where the use of OMWW can affect the growth of saprophytic fungi, the incidence of foliar diseases, and the growth of soil-borne pathogens before and after harvest in strawberries and in tulip plants. Furthermore, in another study has been reported that substances extracted from OMWW showing antifungal action inhibited the mycelium growth of Fusarium solani and R. solani [94].…”

Effectiveness of Olive Fruit Polyphenol Extract Combined with Aqueous Extracts of Solid Wastes of Pomegranate or/ and Orange Juice Against Important Plant Pathogens - Part 2 (in vivo studies)

“…Regarding the use of OMWW, it is well known that OMWW are the main pollutant parameter responsible for the severe environmental impact of wastewater mills especially in the Mediterranean countries. However, several research studies have demonstrated the potential use of these compounds as environmental friendly plant protective method applied on several plant species producing fruits, vegetables and flowers during the growing season and postharvest period under storage conditions [68][69][70][71]. Furthermore, Kotsou et al, [72] reported that OMWW shown antifungal action against R. solani in field experiments while Mavrakis,[73] also reported that various forms of OMWW polyphenols and especially the liquid one affect the development of several important plant pathogenic fungi such as B. cinerea, A. alternata, F. oxysporum f.sp., melonis, Rhizopus species, C. higginsianum, and P. parasitica var.…”

Olive Mill Wastes: A Source of Bioactive Molecules for Plant Growth and Protection against Pathogens

“…The strong fungicidal activity of water extracts of WPO, diluted 1:10, was demonstrated against Phytophthora capsica [139]. The filter sterilized OMWW also inhibited the in vitro mycelium growth of B. tulipae, F. oxysporum, Aspergillus niger, and Penicillium spp [140].…”