Effect of olive oil on the production of mycelial biomass and polysaccharides of Grifola frondosa under high oxygen concentration aeration

Hsieh, Chienyan; Liu, Chia-Jang; Tseng, Mei-Hua; Lo, Chaur-Tsuen; Yang, Yuan-Chang

doi:10.1016/j.enzmictec.2005.11.033

Cited by 49 publications

(31 citation statements)

References 19 publications

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“…The residual reduced sugar (RRS) in culture broth was determined by HPLC (Shimadzu LC-10AS) with an ionic exchange column [20]. The broth was centrifuged at 10,290g (10,000 rpm) for 10 min, and the supernatant was collected and filtered through 0.45 lm cellulose acetate filter.…”

Section: Determination Of Glucose Concentrationmentioning

confidence: 99%

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Chen

et al. 2010

Bioprocess Biosyst Eng

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This work aimed to develop the submerged cultivation conditions for improved exopolysaccharides (EPS) production by Armillaria luteo-virens Sacc. The effects of culture temperature, aeration rate, inoculum level, initial pH, and additives on EPS formation and mycelial growth are investigated. The aeration rate, initial pH, and inoculum level significantly affected EPS production under the submerged cultivation. The developed conditions were as follows: cultivation temperature 23 °C, initial pH 5.0, aeration rate 0.5 vvm, 0.5% Tween 80, inoculum level 5% (v/v), and shaking speed 120 r/min. Under the developed conditions, the highest EPS production was 13.01 g/L at 5 days culture time. EPS production was examined in a 5 L bioreactor, and an unstructured kinetic model for EPS formation was well developed. The verified investigations in the large-scale cultivation system showed that the developed models are able to predict the submerged cultivation process of EPS formation. Current results revealed that the submerged cultivation conditions can be utilized to control EPS production, and the unstructured models developed are suitable for explaining EPS production by A. luteo-virens Sacc QH in a large-scale cultivation bioreactor.

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Section: Determination Of Glucose Concentrationmentioning

confidence: 99%

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Chen

et al. 2010

Bioprocess Biosyst Eng

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“…3. It is well known that vegetable oils exhibit significant stimulatory effects on both mycelial biomass and EPS productions in many mushrooms cultures [15,18,23]. However, it was of interest to find that all the oils exhibited negative effects on the mycelial biomass and EPS productions in G. umbellata culture.…”

Section: Vegetable Oils Additionmentioning

confidence: 97%

“…In this study, in order to search for a proper additive to accelerate mycelia biomass and EPS productions in G. umbellata culture, the additives with stimulatory effects [14][15][16][17][18][19], including organic acids, alcohols, vegetable oils, surfactants and polymers were employed to test their stimulatory effects on both mycelia growth and EPS production in G. umbellata culture. Meanwhile, the effective additive for mycelia growth and EPS production was selected and the cultivation conditions were investigated and discussed.…”

Section: Introductionmentioning

confidence: 99%

The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata

Chen

Huang

Chen

et al. 2009

Bioprocess Biosyst Eng

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In this study, various additives including organic acids, alcohols, vegetable oils, surfactants and polymers were added in the cultural medium to investigate their stimulatory effects on Grifola umbellate mycelia growth and exopolysaccharide (EPS) production. It was found that the commonly used stimulatory additives, effective in other mushrooms' cultures, exhibited negative results in Grifola umbellata submerged culture. In contrast, the polymer additive, polyethylene glycol (PEG), displayed an effective stimulatory effect on both biomass and EPS productions. With the addition of PEG8 (molecular weight: 8,000 Da), the mycelial biomass production at day 12 was increased from 4.69 to 6.30 g/L, accounting for a 34% increase. Meanwhile, the EPS production was enhanced from 0.478 to 0.767 g/L, accounting for 60% increase.

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“…When choosing the type of vegetable oil to be used as inductor of polysaccharide production, it is necessary to take into account the fatty acid composition of the oil since several researchers have reported that important amounts of linoleic acid suppress the production of biomass and polysaccharides, while the presence of oleic promotes their production (Park et al 2002;Hsieh et al 2006Hsieh et al , 2008. Soy and olive oils have been used as promoters of cell growth and polysaccharide production.…”

Section: Submerged Fermentation For Production Of Fungal Polysaccharidesmentioning

confidence: 99%

Polysaccharide Production by Submerged Fermentation

Montoya

Vargas

2015

Polysaccharides

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Effect of olive oil on the production of mycelial biomass and polysaccharides of Grifola frondosa under high oxygen concentration aeration

Cited by 49 publications

References 19 publications

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata

Polysaccharide Production by Submerged Fermentation

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