Effect of Omental Angiogenic Lipid Factor on Revascularization of Autotransplanted Spleen in Dogs

Levy, Yael; Miko, Iren; Hauck, M.; Mathesz, K.; Furka, I.; Orda, Ruben

doi:10.1159/000008569

Cited by 18 publications

(12 citation statements)

References 20 publications

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“…In mongrel dogs, we found that autotransplanted spleen chips need at least 2.5-4 months to regenerate; until this point they behaved as an asplenic or hyposplenic state. 4,6,13 In this study, we found that spleen autotransplantation in mice restored normal function in this regard within 6 weeks.…”

Section: Discussionsupporting

confidence: 56%

“…13,16 The omentum could help angiogenesis, probably by vascular endothelial growth factor (VEGF) expressed mainly by omental adipocytes, rather than stromal-vascular cells. 15 Sasaki demonstrated by scan- ning electron microscopy that neovascularization in rat splenic autografts transplanted into the omentum is classified into four steps: 1) capillarization in the connective tissue surrounding the degenerated autograft, 2) connection of blood vessels between the still-surviving splenic cords and capillaries, 3) rebuilding of splenic sinuses, and 4) remodeling of regenerated blood vessels.…”

Section: Discussionmentioning

confidence: 99%

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Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: Preliminary results

et al. 2003

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Using a spleen autotransplantation model, we conducted hematological, hemorheological, immunological, and morphological studies in mice 6 weeks after splenectomy. Sixty male and female A/J inbred mice were equally divided into 3 groups: 1) SE group, splenectomy was performed; 2) AU group, spleen chips were autotransplanted into the omentum without vascular anastomosis following splenectomy; and 3) C group (controls), no intervention in these mice. At postoperative week 6, the following studies were performed: 1) measurement of hematological parameters; 2) hemorheological studies, including relative cell transit time (RCTT) and fibrinogen levels; and 3) activity of peripheral phagocytes, measured by zymozan-induced chemiluminescence, which was calculated in stimulation index values (SI). In addition, histological investigations of autotransplants were conducted. Erythrocyte mean cell volume and platelet counts, RCTT, fibrinogen levels, and activity of phagocytes were significantly higher in the SE group, compared to those in the C group. In the AU group, these parameters were similar to those in the C group. Morphologically, the transplanted spleen showed normal histology. These data indicate that the transplanted spleens restored their function. We conclude that spleen autotransplantation reserves the normal morphology of spleen and restores most of the spleen's hematological, hemorheological, and immunological functions. Both SI index and erythrocyte deformability can be an informative detection of decreasing splenic function. These data suggest that spleen autotransplantation may provide a useful tool to prevent complications following splenectomy in a clinical setting.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: Preliminary results

et al. 2003

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“…Prevascularization of the patch and rapid anastemosis of the patch vasculature with host myocardial vessels would ensure the rapid engraftment and integration of the patch into the infarcted myocardium. Implantation on omentum for inducing vasculogenesis in tissue explants was previously explored (16)(17)(18). Here, we have used the omentum for prevascularization and cardiac tissue engineering given that this tissue is enriched with blood vessels.…”

Section: Scar) (A and B) Nonpaced (Spontaneous) Electrical Signals Rmentioning

confidence: 99%

Prevascularization of cardiac patch on the omentum improves its therapeutic outcome

Dvir¹,

Kedem²,

Ruvinov³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

321

252

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The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.cardiac tissue engineering ͉ myocardial infarction ͉ SDF-1 ͉ vascularization ͉ affinity-binding alginate scaffolds

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“…9,10 Our study compared the multiple organ-chip model to a single liver, kidney, spleen, and intestine chip technique. The histological data revealed no difference: The spleen appeared to be the most preserved organ in our experimental model, as it had a recognizable structure without significant damage.…”

Section: Discussionmentioning

confidence: 99%

Multiorgan transplantation with a new organ‐chip technique in mice: Preliminary histological data

et al. 2003

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A simple model was developed for multiorgan liver-kidney-spleen-intestine transplantation on 108 inbred mice. Donor operations included hepatectomy, nephrectomy, splenectomy, and jejunum segment resection. Following removal of the organ, small slices or abdominal organ "chips" were prepared. During multiorgan recipient operations, chips from each of these organs were transplanted into the omentum; in the control single-organ groups, only 1 organ was transplanted. All animals survived. Biopsies were taken for histology after 6 weeks. All organs were found to have developed a blood supply. In the liver chips, hypertrophied cells could be detected. In the margin of the kidney tissue, both the glomeruli and tubules were preserved. Lymphoid zone and red pulp were intact in spleen chips. All layers of the intestinal chips were identifiable and contained intraluminal mucinous substances. This model is a simple surgical intervention with the possibility of the investigation of 4 organs.

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Effect of Omental Angiogenic Lipid Factor on Revascularization of Autotransplanted Spleen in Dogs

Cited by 18 publications

References 20 publications

Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: Preliminary results

Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: Preliminary results

Prevascularization of cardiac patch on the omentum improves its therapeutic outcome

Multiorgan transplantation with a new organ‐chip technique in mice: Preliminary histological data

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