Effect of one minute exposure to light at night on rat pineal serotonin <i>N</i>‐acetyltransferase and melatonin

Illnerová, Helena; Vanĕcek, J; Krecek, J; Wetterberg, Lennart; Sääf, Jan

doi:10.1111/j.1471-4159.1979.tb00407.x

Cited by 98 publications

(20 citation statements)

References 16 publications

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“…Light pulses are known to instantly suppress the nocturnal melatonin secretion in the rodent pineal tissue (20,27). When the animals in our experiment were exposed to a 15-min light pulse at circadian time (CT) 20, dialysate melatonin levels significantly decreased to basal levels within 2 hr after the start of light exposure (t ϭ 5.13, df ϭ 4, P Ͻ 0.01) (Fig.…”

Section: Circadian Melatonin Secretion Is Clock-controlled and Synchrmentioning

confidence: 80%

Bimodal circadian secretion of melatonin from the pineal gland in a living CBA mouse

Nakahara

Nakamura

Iigo

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Circadian melatonin secretion is the best-known output signal from the circadian pacemaker in the suprachiasmatic nucleus that indicates internal conditions of the body. We have established a system that enables long-term monitoring of melatonin secretion by implanting a transverse microdialysis probe in or near the pineal gland in a freely moving mouse. This in vivo method enabled continuous measurement of melatonin secretion over a period of >20 days in individual CBA mice, with simultaneous recording of the locomotor activity. Pineal melatonin secretion was completely matched to the circadian change of locomotor activity, and for the light-induced phase shift, the shift of melatonin secretion was clearer than the shift of locomotor rhythm. This analysis allowed us to detect rhythm with a high sensitivity: two peaks of daily secretion were observed, with the first small peak at the day-night transition time and the second large peak at midnight. The large nighttime peak was suppressed by tetrodotoxin, a Na ؉ channel blocker, and enhanced by both phenylephrine and isoproterenol, ␣-and ␤-adrenergic agonists, whereas daytime melatonin levels were not affected by tetrodotoxin infusion. This finding suggests that, in CBA mice, melatonin release at night is activated by adrenergic signaling from the superior cervical ganglion, but the enhancement of melatonin during daytime is not mediated by neuronal signaling.circadian rhythm ͉ locomotor activity ͉ microdialysis I n mammals, most physiological and behavioral events are subject to well controlled daily oscillations, and these rhythms are generated by the circadian clock in the suprachiasmatic nucleus (SCN) (1, 2). Recent progress in the genetic dissection of the clock genes has provided an outline of the molecular machinery of clock oscillation: the generation of rhythm begins at the transcription͞ translation feedback loops of clock genes (3-6). The dynamics of this core oscillatory loop is now monitored in transgenic animals carrying the mPer1 promoter driven luciferase reporter (7-9).From the Drosophila to mammals, an astonishing feature in circadian biology is that the rhythm of this gene transcription is integrated to cell, tissue, and organ, and finally to coordinated temporal organization at the system level. Because the molecular monitoring of gene transcription is now available, real-time rhythm-effector analysis is inevitable to clarify the linkage from the molecular oscillator to the effector. Of the circadian effectors, the behavioral sleep-awaking rhythm is the best known, and locomotor activity is used as a noninvasive output showing the clear-cut circadian expression in living mice. However, the behavioral rhythm is only one output of the SCN oscillator, and the real-time nature of the time information with regard to the body system has not been previously addressed.In our search for a measure of the circadian output that indicates internal conditions of the body, we focused on melatonin (N-acetyl5methoxytryptamine), a hormone primarily secreted by t...

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Section: Circadian Melatonin Secretion Is Clock-controlled and Synchrmentioning

confidence: 80%

Bimodal circadian secretion of melatonin from the pineal gland in a living CBA mouse

Nakahara

Nakamura

Iigo

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…Effect of Light Exposure at Night. Acute light exposure at night induces a rapid and complete inhibition of AA-NAT activity and MEL synthesis in the rat pineal gland Illnerova et al, 1979). A 1-min light pulse is sufficient to reduce AA-NAT activity and MEL concentrations to daytime values within 20 min Drijfhout et al, 1996c).…”

Section: Mechanisms Involved In the Termination Of Nocturnal Melatonimentioning

confidence: 98%

“…In addition, the activity of these three structures is directly (IGL, raphe) or indirectly (PVN) modulated by light. Since short light exposure at night induces a very rapid diminution of MEL synthesis and release Illnerova et al, 1979;Drijfhout et al, 1996c), it is possible that the central pathway involved in this rapid light inhibition (Dafny, 1980) that the central IGL-pineal pathway could be involved in the rapid inhibitory effect of a light flash on the metabolic activity of the pineal gland (Cipolla-Neto et al, 1995;Bartol et al, 1997).…”

Section: Multiple Regulation Of Pineal Melatonin Synthesis In Mammalsmentioning

confidence: 99%

Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters

2003

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“…Fís/UEM, v. 26, n. 1, p. 97-106, 1. trim. 2015 noturno (vigília do rato) é capaz de reduzir cerca de 30% a atividade da enzima n-acetiltransferase da glândula pineal (ILNEROVÁ et al, 1979). Tal condição poderia influenciar na produção e liberação da melatonina, causando dificuldades no controle do ritmo circadiano de inúmeras variáveis fisiológicas capazes de influenciar a adequada execução do exercício físico.…”

Section: Introductionunclassified

Horário do dia, luminosidade ambiental e exercício físico sobre parâmetros inflamatórios e de performance em ratos nadadores

2015

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RESUMOO modelo animal é utilizado em inferências de muitos mecanismos fisiológicos ao modelo humano, entretanto, investigações tem negligenciado o completo controle ambiental e cronobiológico. O objetivo deste estudo foi analisar respostas inflamatórias decorrentes do exercício físico realizado até a exaustão em intensidade individualmente determinada na transição aeróbia/anaeróbia em diferentes horários do dia e luminosidades ambientais. Ratos Wistar foram submetidos a dois tipos de luminosidade ambiental durante o período noturno: luminosidade padrão (LP; escuridão total) ou experimental (LE; >600nm; <15lux). Os animais foram avaliados pelo teste de lactato mínimo e então submetidos a um exercício exaustivo nesta intensidade (TE).Todos os procedimentos experimentais foram realizados às 12:00h (período de repouso) ou 20:00h (vigília). TE não sofreu influência do horário ou luminosidade, porém, ratos avaliados sob LP às 20:00h apresentaram menores valores para marcadores inflamatórios, mostrando a importância deste controle em experimentos que envolvam animais noturnos.Palavras-chave: Cronobiologia. Performance. Ratos. Inflamação. Luminosidade ambiental.

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Effect of one minute exposure to light at night on rat pineal serotonin N‐acetyltransferase and melatonin

Cited by 98 publications

References 16 publications

Bimodal circadian secretion of melatonin from the pineal gland in a living CBA mouse

Bimodal circadian secretion of melatonin from the pineal gland in a living CBA mouse

Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters

Horário do dia, luminosidade ambiental e exercício físico sobre parâmetros inflamatórios e de performance em ratos nadadores

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