Effect of oral Bacillus coagulans administration on the density of vancomycin-resistant enterococci in the stool of colonized mice

Donskey, Curtis J.; Hoyen, Claudia K.; Das, Sarbani M.; Farmer, Sean; Dery, MarkAlain

doi:10.1046/j.1472-765x.2001.00948.x

Cited by 41 publications

(28 citation statements)

References 18 publications

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“…The VRE intestinal colonization experimental model was adapted from Donskey et al (7). Daily subcutaneous administration of clindamycin (1.4 mg/day) for 5 days was used to disrupt the intestinal microbiota as necessary to induce the VRE infection.…”

Section: Methodsmentioning

confidence: 99%

“…No recurrence was observed. Donskey et al (7) showed that a strain of Bacillus was able to reduce the fecal VRE concentration, but they did not implicate the production of a bacteriocin. Although the bacteriocin-producing strains are able to reduce the densities of VRE populations following an infection facilitated by clindamycin, these strains have no effect on the total Enterococcus spp.…”

Section: Fig 4 Changes In the Densities Of Total Vancomycin-resistantmentioning

confidence: 99%

See 1 more Smart Citation

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Millette

Cornut

Dupont

et al. 2008

Appl Environ Microbiol

139

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This study demonstrated the capacity of bacteriocin-producing lactic acid bacteria (LAB) to reduce intestinal colonization by vancomycin-resistant enterococci (VRE) in a mouse model. Lactococcus lactis MM19 and Pediococcus acidilactici MM33 are bacteriocin producers isolated from human feces. The bacteriocin secreted by P. acidilactici is identical to pediocin PA-1/AcH, while PCR analysis demonstrated that L. lactis harbors the nisin Z gene. LAB were acid and bile tolerant when assayed under simulated gastrointestinal conditions. A well diffusion assay using supernatants from LAB demonstrated strong activity against a clinical isolate of VRE. A first in vivo study was done using C57BL/6 mice that received daily intragastric doses of L. lactis MM19, P. acidilactici MM33, P. acidilactici MM33A (a pediocin mutant that had lost its ability to produce pediocin), or phosphate-buffered saline (PBS) for 18 days. This study showed that L. lactis and P. acidilactici MM33A increased the concentrations of total LAB and anaerobes while P. acidilactici MM33 decreased the Enterobacteriaceae populations. A second in vivo study was done using VRE-colonized mice that received the same inocula as those in the previous study for 16 days. In L. lactis-fed mice, fecal VRE levels 1.73 and 2.50 log 10 CFU/g lower than those in the PBS group were observed at 1 and 3 days postinfection. In the P. acidilactici MM33-fed mice, no reduction was observed at 1 day postinfection but a reduction of 1.85 log 10 CFU/g was measured at 3 days postinfection. Levels of VRE in both groups of mice treated with bacteriocin-producing LAB were undetectable at 6 days postinfection. No significant difference in mice fed the pediocin-negative strain compared to the control group was observed. This is the first demonstration that human L. lactis and P. acidilactici nisin-and pediocin-producing strains can reduce VRE intestinal colonization.

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Section: Methodsmentioning

confidence: 99%

Section: Fig 4 Changes In the Densities Of Total Vancomycin-resistantmentioning

confidence: 99%

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Millette

Cornut

Dupont

et al. 2008

Appl Environ Microbiol

139

View full text Add to dashboard Cite

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“…The stools were collected periodically at six hour intervals up to 48 hours and subjected for enterococci colonization density study. 9 …”

Section: Model-dependent Methodsmentioning

confidence: 99%

“…7 To be therapeutically active, probiotics must arrive in the intestine alive and in sufficient numbers which is suggested at 10 6 -10 storage requirements during transportation and storage. [7][8][9][10][11][12] After their consumption, the hydrolytic enzymes, the acidic conditions of stomach, and the bile salts in the gastrointestinal (GI) tract also adversely affect the viability of probiotics. 7,[12][13][14][15] Various approaches such as the addition of different growth promoters, manipulation of fermentation and storage conditions of the food carriers, careful selection of the culture organisms according to their interrelationships and acid-bile resistance, and employing microencapsulation technology have been investigated to improve the amount of viable cells arriving in the intestine.…”

Section: Introductionmentioning

confidence: 99%

Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres

Alli¹

2011

IJN

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Development of a novel delivery system has been attempted to deliver viable probiotic cells into the gut for a prolonged period of time while maintaining high numbers of viable cells within the formulation throughout the shelf-life of the product and during the gastrointestinal transit. Core mucoadhesive microspheres of Bacillus coagulans were developed employing several grades of hypromellose, a mucoadhesive polymer, following coacervation and phase separation technique and were subsequently enteric-coated with hypromellose phthalate. Microspheres were evaluated for percent yield; entrapment efficiency; in vitro swelling; surface morphology; particle size, size distribution, and zeta potential; flow property, mucoadhesion property by the ex vivo mucoadhesive strength test and the in vitro wash off test; in vitro release profile and release kinetic; in vivo probiotic activity; and stability. The values for the kinetic constant and regression coefficient of model-dependent approaches and the difference factor ( f 1 ), the similarity factor ( f 2 ), and the Rescigno index ( ξ 1 and ξ 2 ) of model independent approaches were determined for comparing in vitro dissolution profiles. Freeze dried B. coagulans cells were successfully formulated as enteric-coated mucoadhesive microspheres with satisfactory physical structure and yield. The viability of B. coagulans was maintained in the simulated gastric conditions and during processing; in simulated intestinal conditions exhibiting mucoadhesion, and controlling and extending the viable cell release following zero-order; and was satisfactorily stable at room temperature. Test results depict statistically significant effects of the hypromellose grade and their concentration on the performance and release profile of formulations.

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“…It is a good candidate for probiotic use, produces organic acids and possesses the capacity to sporulate. It secretes a bacteriocin, coagulin, which has activity against a broad spectrum of enteric microbes [15][16][17]. Bacillus subtilis is also a Gram-positive, lactic acid-forming bacterial species.…”

Section: Introductionmentioning

confidence: 99%

Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

Bhavan¹

2018

Insights Biol Med

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The probiotic effects of Bacillus coagulans and Bacillus subtilis were studied on survival, growth, concentrations of basic biochemical constituents, activities of digestive enzymes, and their colony establishments in the gut of Macrobrachium rosenbergii post-larvae (PL). Eleven groups of PL (2.03±0.05 in length and 0.18±0.01g in weight), each consists of 35 individuals maintained in 25 L of ground water and fed ad libitum with fi ve serially diluted concentrations, 10 , 10 -7 and 10 -9 of B. coagulans, and B. subtilis incorporated diets containing 40% protein, for 45 days. Diet without incorporation of any of these probiotics was served as control. These probiotics were found to be alive in the respective feed even on day-15 after their formulations. Signifi cant improvement in survival, nutritional indices (weight gain, specifi c growth rate, food conversion ratio and protein effi ciency ratio), contents of basic biochemical constituents (total protein, amino acid, carbohydrate and lipid) and activities of digestive enzymes (protease, amylase and lipase) were observed (P<0.05), particularly in 10 -7 concentration of B. coagulans, and B. subtilis incorporated diets fed PL when compared with control. The biochemical confi rmation tests revealed that presence of Escherichia coli, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., in the gut of control PL. In the gut of PL fed with B. coagulans incorporated diet, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., were found to be competitively excluded, whereas, in the gut of PL fed with B. subtilis incorporated diet, Acetonobacter sp., and Salmonella sp., only were found to be excluded competitively. Actually, colonies of Bacillus sp., and Lactobacillus sp., were found to be establishment in the gut of PL fed with B. coagulans, and B. subtilis incorporated diets. Overall, these probiotics incorporated diets produced better growth and survival due to better FCR and activities of digestive enzymes, which in turn led to better nutritional profi le. Therefore they are recommended as feed additives for sustainable culture of M. rosenbergii.

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Effect of oral Bacillus coagulans administration on the density of vancomycin-resistant enterococci in the stool of colonized mice

Cited by 41 publications

References 18 publications

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres

Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

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