Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair

Abstract

This study evaluated the effects of oral mucosal transplantation on epidermal growth factor (EGF) and vascular endothelial growth factor C (VEGF-C) in skin wound repair. Sixty-four rats were randomly separated into group A, B, C and D (16 rats in each group). The right abdomen skin was excised 1, 3, 5 and 7 days after injury, respectively. Oral mucosa of the rat tongue was transplanted to the right abdomen skin. Fourteen days after the healing of the oral mucosa graft, the rat skin full-thickness model was prepared at the transplant site (the study group) and the contralateral site (the control group). Rats in each group were anesthetized and sacrificed at 1, 3, 5 and 7 days after injury. Expression of EGF and VEGF-C in skin tissue was detected by RT-qPCR and ELISA. At 3 days, expression levels of EGF and VEGF-C mRNA and protein in skin tissue were significantly higher than those at 1 day (P<0.05). At 5 days, expression levels of EGF and VEGF-C mRNA and protein in skin tissue were significantly higher than those at 3 days (P<0.05). At 7 days, expression levels of EGF mRNA and protein in skin tissue were significantly lower than those at 5 days (P<0.05), while VEGF-C levels were significantly increased (P<0.05). Expression levels of EGF and VEGF-C mRNA and protein in the skin tissue of the study group were significantly lower than those in the control group at all days (P<0.05). EGF and VEGF-C may be involved in scar formation, and play an important role in the process of skin wound repair.

Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway

“…The activated PI3K/Akt/mTOR pathway not only upregulates the expression of VEGF, FGF and EGF, but also promotes cell growth and migration, angiogenesis and collagen synthesis, induces EMT and stimulates wound healing [35,36]. EGF and VEGF-C may be involved in scar formation, and stimulate the process of skin wound repair [37]. In the wounds in STZinduced diabetic mice, suppressed levels of inflammationrelated cytokines, tumor necrosis factor-α (TNF-α), IL-8, and IL-6 were achieved after the treatment of an antiinflammation drug [38].…”

3D printing-mediated microporous starch hydrogels for wound hemostasis

“…VEGF is mainly expressed in endothelial cells, and its levels gradually increase from the early stage to the middle stage of skin wound healing. 39 As shown in Fig. 5(c) and (d).…”

Polymorphism analysis of candidate risk genes for pressure injuries in older Japanese patients: A cross‐sectional study at a long‐term care hospital

“…VEGFC is secreted by macrophages under inflammatory conditions 17 . VEGFC binds to the VEGF receptor 3, which is mainly expressed by lymphatic endothelial cells, and specifically promotes lymphangiogenesis 33 . Recently, the relationship of rs1485766 with several disease risks was investigated in five studies (Table 7).…”