1993
DOI: 10.1016/0308-8146(93)90142-3
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Effect of organic solvent and the lipid content on functionality of bovine lung protein isolates

Abstract: Protein was isolated from bovine lung and extracted with organic solvents of varying polarities. Lipid represented 20% by weight of the total isolate, and the amount extracted depended on the polarity of the organic solvents employed. Phospholipids, cholesterol and free fatty acids were the lipid classes detected, phospholipids being the major component left after solvent extraction. Water absorption characteristics, solubility of the protein and superficial hydrophobicity were related to the amount of lipids … Show more

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Cited by 3 publications
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“…Other than the solvent cost, requirement of explosion‐proof facilities and equipment, and negative environmental impact, the major drawback of solvent de‐oiling is the destabilizing effect it has on the tertiary‐structural confirmation of protein (Arêas et al, 1993). Utilizing organic solvents is often associated with protein denaturation due to solubilizing non‐polar side chains of amino acid residues and weakening the hydrophobic interactions in protein (Damodaran, 2017).…”
Section: Resultsmentioning
confidence: 99%
“…Other than the solvent cost, requirement of explosion‐proof facilities and equipment, and negative environmental impact, the major drawback of solvent de‐oiling is the destabilizing effect it has on the tertiary‐structural confirmation of protein (Arêas et al, 1993). Utilizing organic solvents is often associated with protein denaturation due to solubilizing non‐polar side chains of amino acid residues and weakening the hydrophobic interactions in protein (Damodaran, 2017).…”
Section: Resultsmentioning
confidence: 99%