Effect of osteogenic protein‐1 on the matrix metabolism of bovine tendon cells

Abstract

Tendon rupture is a common sports injury in adults. However, the mechanical properties of repair tissue are inferior to those of normal tissue. To accelerate tendon healing, an in vivo approach using growth factors has been applied and has shown evidence for the efficacy of biological stimulation of the repair process. Recombinant human osteogenic protein-1 (rhOP-1) has been shown to be effective in stimulating matrix production by various connective tissues. To test the effect of rhOP-1 on the matrix metabolism of tendon cells in vitro, bovine tendon cells were cultured in monolayer with various doses of rhOP-1 for 7 days. The addition of rhOP-1 to cell culture media resulted in significant increases in cell proliferation, DNA content, and the synthesis of proteoglycans (PGs) and collagen, compared to control cultures. The relative percentage of large PGs in the OP-1 culture was higher than that in the control culture. In conclusion, we show for the first time that rhOP-1 stimulates the proliferation of tendon cells and their ability to synthesize and accumulate PGs and collagen in their extracellular matrix. These biological properties may be used in the tissue-engineering of tendon tissues. ß

Bone morphogenetic protein‐7 regulates differentially the mRNA expression of bone morphogenetic proteins and their receptors in rat achilles and patellar tendon cell cultures

“…The current results show that BMP-7 stimulated moderately the proliferation of Achilles and Patellar cells. By comparison, a recent study also showed that OP-1 (BMP-7) stimulated cell proliferation, DNA content, synthesis of collagen and proteoglycans in bovine tendon cell cultures [Yamada et al, 2008]. BMP-2, which was previously shown to enhance tendon healing in vivo, did not stimulate proliferation of two clonal Achilles tendon cell lines [Salingcarnboriboon et al, 2003].…”

BMPs are mediators in tissue crosstalk of the regenerating musculoskeletal system

“…BMP7 triggers the production of fibrous tissue between tendon and bone comparable with native regeneration processes in the tissue-connecting area (Rodeo et al 1993;Gerber et al 1999). BMP7 also increases the proliferation and expression of tenocyte markers such as CollagenI and proteoglycans in a dose-dependent manner (Yamada et al 2008). In gene therapy studies of BMP2-overexpressing rat cells embedded in calcium alginate gels, a positive effect on tendon-bone healing has been observed in rabbits after anterior cruciate ligament reconstructions.…”

Are BMPs Involved in Normal Nerve and Following Transection?: A Pilot Study

“…Until recently, although various neurotrophic factors were reported, nerve growth factor (NGF), glial cell-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3/4 (NT-3/4) were considered important molecules in these situations and decreased axonal degeneration and neuronal apoptosis [8,18,26]. Recent studies demonstrate recombinant human BMPs are capable of promoting regeneration in various tissues other than bone such as tendon or cartilage [7,31] and that they also possess neurotrophic functions, such as regulation of neuronal survival and differentiation [21,28,29].…”