Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs

Jin, Jichun; Jin, Xinglin; Zhang, Xian; Piao, Yingshi; Liu, Shuangping

doi:10.3892/mmr.2013.1428

Cited by 24 publications

(21 citation statements)

References 27 publications

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“…OSW-1 is a natural compound that has shown considerable anticancer activity in several kinds of cancer cells, including pancreatic cancer, ovarian cancer, leukemia, human hepatomas, and malignant brain tumors (5,39). Herein we showed that OSW-1 can also inhibit colon cancer cells more powerfully than other drugs.…”

Section: Discussionmentioning

confidence: 74%

Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

et al. 2017

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Abstract. As a natural compound, Ornithogalum caudatumAit is primarily used as an anti-inflammatory and antitumor agent in Chinese folk medicine. In 1992, OSW-1 was isolated from this compound, which is a new member of cholestane saponin family. In numerous recent studies, OSW-1 has been shown to have powerful cytotoxic anticancer effects against various malignant cells. However, the therapeutic efficacy of OSW-1 on colon cancer and the underlying mechanism are not understood. To explore the mechanism underlying OSW-1 in antitumor therapy, a therapeutic function analysis of OSW-1 on colon cancer was performed in vitro and in vivo. It was shown that with low toxicity on normal colonic cells, OSW-1 suppresses colon cancer cells in vitro and this inhibition was via the intrinsic apoptotic pathway, which increased cellular calcium, changed mitochondrial membrane potential, disrupted mitochondrial morphology, and led to the release of cytochrome c and the activation of caspase-3. Furthermore, in a nude mouse model, OSW-1 had a powerful effect on suppressing colon tumor proliferation without significant side effects through the apoptosis pathway. Taken together, these results demonstrate that OSW-1 is a potential drug for colon cancer treatment.

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Section: Discussionmentioning

confidence: 74%

Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

et al. 2017

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“…All of the signaling pathways and biological processes that the putative target genes are involved in are concerned with adipocyte differentiation and metabolism, so we hypothesized that miR-1908 would have an effect on the differentiation and metabolism of human adipocytes. Additionally, miR-1908 has been identified as having a close relationship with the processes of metastatic invasion, angiogenesis, and colonization of melanomas [22]; it may be involved in the malignant progression of chordoma [33]; and may participate in the formation of hepatoma cells [34].…”

Section: Discussionmentioning

confidence: 99%

The biological effects of hsa-miR-1908 in human adipocytes

et al. 2014

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MicroRNAs (miRNAs) are small non-coding RNAs involved in the regulation of gene expression. MiR-1908 is a recently identified miRNA that is highly expressed in human adipocytes. However, it is not known what role of miR-1908 is involved in the regulation of human adipocytes. In this study, we demonstrate that the level of miR-1908 increases during the adipogenesis of human multipotent adipose-derived stem (hMADS) cells and human preadipocytes-visceral. Overexpression of miR-1908 in hMADS cells inhibited adipogenic differentiation and increased cell proliferation, suggesting that miR-1908 is involved in the regulation of adipocyte cell differentiation and metabolism, and, thus, may have an effect on human obesity.

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“…MiR-299-5p was downregulated in patients with t (11q23)/MLL-rearrangement and upregulated in t (15:17) cytogenetic subtypes [35]. Another study on hepatoma cells showed that hsa-miR-299-5p targeted osteopontin (OPN), which is known to be essential for enhancing proliferation and tumorigenicity [36]. Gain- and loss-of-function experiments revealed that miR-299-5p plays a role in the regulation of hematopoietic progenitor; it may therefore act as miRNA in the differentiation of megakaryoblasts [37].…”

Section: Discussionmentioning

confidence: 99%

Importance of miR-299-5p in colorectal cancer

Fateh

Feizi

Safaralizadeh

et al. 2017

aog

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BackgroundMicroRNAs (miRNAs) are effective regulators of gene expression that play a pivotal role in the pathogenesis of colorectal cancer (CRC) and various other cancers. The high prevalence of aberrant miRNA expression in CRC suggests that they can be used as biomarkers and anticancer molecules for therapeutic purposes. There is evidence that microRNA-299-5p (miR-299-5p) is associated with vital cell processes (e.g. epithelial-mesenchymal transition, proliferation, and tumorigenicity) and its improper expression with tumorigenesis in many types of human cancer. This prospective study investigated the contribution of miR-299-5p to CRC tumorigenesis.MethodsThe real-time reverse transcription-polymerase chain reaction was used to examine miR-299-5p expression levels prospectively in 40 sample pairs of CRC tissue and adjacent noncancerous tissue (>2 cm from cancer tissue). The ability of miR-299-5p to function as a tumor marker was also examined.ResultsThe expression levels of miR-299-5p were significantly downregulated in the group of CRC samples compared with matched noncancerous tissue samples. No significant relationship was found between miR-299-5p expression levels and clinicopathological features. Receiver operating characteristic analysis gave an area under the curve of 71% for miR-299-5p with 68% sensitivity and 78% specificity (P=0.001).ConclusionThe miRNA miR-299-5p may be considered as a tumor marker in CRC and could be of assistance as a potential predictive biomarker in the diagnosis of this cancer.

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Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs

Cited by 24 publications

References 27 publications

Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

The biological effects of hsa-miR-1908 in human adipocytes

Importance of miR-299-5p in colorectal cancer

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