Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection

Abstract

We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.

The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice

“…A number of scholars have previously demonstrated that C57Bl/6 and CBA mice have opposite properties. The differences consisted in varied reactivity of the immune system, the system of mononuclear phagocytes, and their regulation [7]. Furthermore, the responses of the immune and hormonal systems of CBA and C57Bl/6 mice to bacterial and fungal agents, M. tuberculosis [8] and C. albicans [1], were also different.…”

“…Furthermore, the responses of the immune and hormonal systems of CBA and C57Bl/6 mice to bacterial and fungal agents, M. tuberculosis [8] and C. albicans [1], were also different. CBA and C57Bl/6 mice are opposite in a number of ways: according to such properties as activity of metabolic processes in the liver [9], sensitivity to infectious agents [10, 11], structural and functional characteristics of the adrenal cortex [9], quantitative representation of cells of the mononuclear phagocyte system in various organs [12], and activation of immune cells [7]. According to the previously described interline differences, it can be assumed that mammals with different genetic programs have different ways of responding to the immune system.…”