Effect of oxygen and glucose availability during in vitro maturation of bovine oocytes on development and gene expression

Whitty, Annie; Kind, Karen L.; Dunning, Kylie R.; Thompson, Jeremy G.

doi:10.1007/s10815-021-02218-w

Cited by 11 publications

(8 citation statements)

References 72 publications

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“…There is a large diversity of IVM protocols in use and different media and conditions might influence mitochondrial configuration to a different degree. It was demonstrated that high oxygen concentrations (20%) result in a higher embryo yield after IVM in bovine compared to low concentrations (5–7%) [ 80 , 81 ]. However, supraphysiological oxygen levels lead to the increased risk of reactive oxygen species (ROS) formation, which in its turn might compromise mitochondrial function.…”

Section: Mitochondria Patterns During Ivmmentioning

confidence: 99%

The Role of Mitochondria in Oocyte Maturation

Kirillova

Smitz

Сухих

et al. 2021

Cells

163

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With the nucleus as an exception, mitochondria are the only animal cell organelles containing their own genetic information, called mitochondrial DNA (mtDNA). During oocyte maturation, the mtDNA copy number dramatically increases and the distribution of mitochondria changes significantly. As oocyte maturation requires a large amount of ATP for continuous transcription and translation, the availability of the right number of functional mitochondria is crucial. There is a correlation between the quality of oocytes and both the amount of mtDNA and the amount of ATP. Suboptimal conditions of in vitro maturation (IVM) might lead to changes in the mitochondrial morphology as well as alternations in the expression of genes encoding proteins associated with mitochondrial function. Dysfunctional mitochondria have a lower ability to counteract reactive oxygen species (ROS) production which leads to oxidative stress. The mitochondrial function might be improved with the application of antioxidants and significant expectations are laid on the development of new IVM systems supplemented with mitochondria-targeted reagents. Different types of antioxidants have been tested already on animal models and human rescue IVM oocytes, showing promising results. This review focuses on the recent observations on oocytes’ intracellular mitochondrial distribution and on mitochondrial genomes during their maturation, both in vivo and in vitro. Recent mitochondrial supplementation studies, aiming to improve oocyte developmental potential, are summarized.

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Section: Mitochondria Patterns During Ivmmentioning

confidence: 99%

The Role of Mitochondria in Oocyte Maturation

Kirillova

Smitz

Сухих

et al. 2021

Cells

163

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show abstract

“…Alterations in metabolic activity are known culture-induced negative effects, and IVM-inflicted perturbations in COC metabolism have been observed in several species [30][31][32][33][34]. Recently, we have shown an impaired precocious increase in glycolytic activity in CCs during the pre-IVM phase of the mouse CAPA-IVM when compared to their in vivo matured counterparts [35], which may contribute to the lower in vitro oocyte competency.…”

Section: Introductionmentioning

confidence: 93%

Effects of lactate, super-GDF9, and low oxygen tension during bi-phasic in vitro maturation on the bioenergetic profiles of mouse cumulus–oocyte complex

Akin

Ates

Mengden

et al. 2023

Biology of Reproduction

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In vitro maturation (IVM) is an alternative assisted reproductive technology (ART) with reduced hormone related side-effects and treatment burden compared to conventional IVF. Capacitation (CAPA)-IVM is a biphasic IVM system with improved clinical outcomes compared to standard monophasic IVM. Yet, CAPA-IVM efficiency compared to conventional IVF is still suboptimal in terms of producing utilizable blastocysts. Previously we have shown that CAPA-IVM leads to a precocious increase in cumulus cell (CC) glycolytic activity during cytoplasmic maturation. In the current study, considering the fundamental importance of CCs for oocyte maturation and cumulus-oocyte complex (COC) microenvironment, we further analyzed the bioenergetic profiles of maturing CAPA-IVM COCs. Through a multi-step approach, we (i) explored mitochondrial function of the in vivo and CAPA-IVM matured COCs through real-time metabolic analysis with Seahorse analyzer; and to improve COC metabolism (ii) supplemented the culture media with lactate and/or super-GDF9 (an engineered form of growth differentiation factor 9) and (iii) reduced culture oxygen tension. Our results indicated that the pre-IVM step is delicate and prone to culture related disruptions. Lactate and/or super-GDF9 supplementations failed to eliminate pre-IVM induced stress on COC glucose metabolism and mitochondrial respiration. However, when performing pre-IVM culture under 5% oxygen tension, CAPA-IVM COCs showed a similar bioenergetic profiles compared to in vivo matured counterparts. This is the first study providing real-time metabolic analysis of the COCs from a biphasic IVM system. The currently used analytical approach provides the quantitative measures and the rational basis to further improve IVM culture requirements.

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“…Oxygen during in vitro embryo culture is an essential chemical factor that improves development from gametes to the blastocyst stage, intervening in their metabolism, genetics and epigenetics [1][2][3][4][5]. In some mammals including humans, the oviduct and uterus oxygen concentrations are between 2-8% [6], showing lower concentrations of oxygen in the uterus [7], thus concentrations close to these should represent a more physiological environment.…”

Section: Oxygen Tension In Embryo Culturementioning

confidence: 99%

Improving Embryo Quality by Strictly Controlling IVF Laboratory Environment

García-Ferreyra¹,

Sánchez-Pavón²

2023

Embryology Update

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In recent years, several changes have been made in different aspects of in vitro fertilization to improve embryo quality and ultimately the clinical outcomes in assisted reproduction technology (ART). These approaches include improvements in air quality inside the lab to ensure VOCs-free air, use of tri-gas incubator and embryo-tested devices and plastics, adequate control of pH and osmolarity of culture media, and strict quality control that allows an adequate development of the embryos until blastocyst stage. Other strategies to improve the embryo quality during in vitro culture include volume reduction of drop culture media, and individual or group culture of embryos. This work summarizes several strategies to improve embryonic quality during their in vitro culture in assisted reproduction procedures.

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Effect of oxygen and glucose availability during in vitro maturation of bovine oocytes on development and gene expression

Cited by 11 publications

References 72 publications

The Role of Mitochondria in Oocyte Maturation

The Role of Mitochondria in Oocyte Maturation

Effects of lactate, super-GDF9, and low oxygen tension during bi-phasic in vitro maturation on the bioenergetic profiles of mouse cumulus–oocyte complex

Improving Embryo Quality by Strictly Controlling IVF Laboratory Environment

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