Effect of Oxygen on the Development of Respiratory Activity in Escherichia Coli

Hino, Seiichi; Maeda, Misuzu

doi:10.2323/jgam.12.247

Cited by 28 publications

(19 citation statements)

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“…E. coli K12 W1895 was grown aerobically for 18 hr in a nutrient broth at 30°, as described by HINO and MAEDA (13). Cells were collected, washed three times with cold 20 mM phosphate buffer (pH 7.2) and suspended in the same buffer.…”

Section: Methodsmentioning

confidence: 99%

Inactivation by oxygen and stabilization by fluorocitrate of aconitase from Escherichia coli.

Hattori

Hino

1981

J. Gen. Appl. Microbiol.

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More than 50% of the total aconitase activity in Escherichia coli was lost when cell-free extracts were prepared under air by a French press, by osmotic rupture of spheroplasts or by sonic treatment, as shown by the fact that the enzyme activity of the extracts prepared by sonic treatment under N2 was more than twice that of the extracts prepared by the other methods. When the extracts prepared by sonic treatment under N2 were gently shaken under air at 25°, about 70 to 80% of the original activity was lost over 30 min, but a constant level of the enzyme activity remained even after prolonged incubation. The remaining aconitase activity after a long aerobic incubation was the same whether the extracts were prepared by sonic treatment under air or N2. Aconitase was stable when cell-free extracts were incubated under anaerobic conditions or when fluorocitrate, in concentrations less than 1 µM, was added during aerobic incubation. Citrate was less effective than fluorocitrate in preventing enzyme inactivation. Aconitase in the extracts, which had been incubated under air with fluorocitrate, was stable against oxygen inactivation and showed different Vmax and Km values from those of the aconitase in the extracts that had been incubated under air without the addition of flourocitrate.Aconitase (citrate (isocitrate) hydro-lyase, EC 4.2

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Section: Methodsmentioning

confidence: 99%

Inactivation by oxygen and stabilization by fluorocitrate of aconitase from Escherichia coli.

Hattori

Hino

1981

J. Gen. Appl. Microbiol.

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“…The media, cultural conditions, and procedures for harvesting and washing the cells were essentially the same as described in detail in the previous paper (1). Aerobic cultures were grown by shaking in a nutrient broth either with or without the addition of 1 % glucose.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous papers (1,2), it was disclosed that low respiratory activity of anaerobically grown cells of Escherichia coli K-12 increased to the level of aerobically grown cells by aeration under conditions that preclude cell division. It was also shown that the change of cellular permeability is one of the causes of this development of respiratory activity.…”

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confidence: 99%

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Biochemical Changes During the Development of Respiratory Activity in Escherichia Coli

Takahashi

Hino

1968

J. Gen. Appl. Microbiol.

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Cell-free extracts were prepared from four types of Escherichia coli cells; cells grown aerobically with glucose, cells grown aerobically without glucose, cells grown anaerobically with glucose, and cells obtained after aeration of the anaerobically grown cells. Examination of the activities of about 20 enzymes in these extracts showed that glucose and oxygen in growth environment generally do not significantly influence the level of enzymes in fermentative pathways. The presence of oxygen during the growth increased the level of formate dehydrogenase, NADH oxidase system, and several enzymes in the tricarboxylic acid cycle, particularly isocitrate dehydrogenase and succinyl-CoA synthetase. The presence of glucose during the growth repressed the level of several enzymes in the glyoxylate cycle, particularly isocitrate lyase and citrate synthase. When thick suspensions of the cells grown anaerobically with glucose were aerated in a glucose-free incubation mixture for 4 hr, initial low activities of about 10 enzymes increased to the level of cells grown aerobically in the absence of glucose, without accompanying significant cell multiplication.

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“…Hino et af. 3) and Takahashi et al 4 ) reported that the aconitase in E. coli was synthesized under the aerobic conditions. Furthermore, Takahashi 6 ) described that in E. coli, the aconitase was induced by the addition of fiuoroacetate, which is well known to act as an inhibitor of the enzyme after conversion to fiuorocitrate.…”

Section: )mentioning

confidence: 99%