Effect of Oxygen Tension on the Developmental Potential of Parthenogenetic Oocytes and Nuclear-Transferred Porcine Oocytes Receiving Fetal Fibroblast Cells.

Abstract

Abstract. The effects of oxygen tension (5% and 20%) during in vitro culture of oocytes and fetal fibroblast cells on the developmental potential of parthenogenetic and nuclear-transferred porcine oocytes were examined. The potential of parthenogenetic oocytes matured and cultured under different oxygen tensions to develop into blastocysts was not significantly different (5 to 16%). The proportions of enucleated oocytes receiving fetal fibroblast cells that developed into blastocysts were significantly lower when somatic cells were cultured under low oxygen tension (2 and 3%) than under high oxygen tension (7%). The effects of oxygen tension during culture of oocytes and somatic cells on the developmental potential of parthenogenetic and nuclear-transferred oocytes is discussed.

Stage-dependent Effect of Leptin on Development of Porcine Embryos Derived from Parthenogenetic Activation and Transgenic Somatic Cell Nuclear Transfer

“…It is believed that its lower efficiency is the result of errors in reprogramming, especially incomplete epigenetic reprogramming [1], which could be improved, to some extent, by modifications of donor preparation, oocyte maturation, activation and embryo culture systems. It has been shown that alteration of oxygen tension, basic culture media or supplementation of certain kinds of growth factors, cytokines, vitamins or amino acids can enhance preimplantational development or quality of cloned embryos [3][4][5][6]. Leptin, a 16KD reproductive hormone and product of the obese gene (ob), which is synthesized in many organs including the reproductive tract, might have a significant direct effect on normal reproduction [7][8][9][10].…”