High oxygen tension during in vitro oocyte maturation improves in vitro development of porcine oocytes after fertilization

Park, J.I.; Hong, Ji Young; Yong, Hae In; Hwang, Woo Suk; Lim, Jeong Mook; Lee, E.S.

doi:10.1016/j.anireprosci.2004.11.002

Cited by 45 publications

(36 citation statements)

References 33 publications

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“…However, we did not find the analogous phenomenon in our study because the rate of meiotic maturation is similar among human exhaled air, 5% O 2 and 20% O 2 in air. Consistent with our results, low oxygen concentration during in vitro maturation (IVM) has no significant effects on nuclear maturation of porcine oocytes (Park et al, 2005). This discrepancy could be due to the diverse maturation mediums or protocols used in the different labs.…”

Section: Discussionsupporting

confidence: 79%

“…Therefore, a stable and reliable incubation system for oocyte maturation in vitro is very important and deserves plenty of attention. In fact, it is discovered that culture conditions during in vitro maturation of oocyte encompass many facets, for example, compositions of culture medium, temperature, humidity, carbon dioxide and oxygen concentrations (Bavister and Poole, 2005;Iwamoto et al, 2005;Park et al, 2005;Swain, 2010;Wrenzycki and Stinshoff, 2013). The majority of studies focuses on the optimization of composition of culture medium used for oocyte maturation in vitro, however, other environmental factors affecting oocyte maturation, such as culture air phase, also need to be examined.…”

Section: Introductionmentioning

confidence: 99%

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Human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development

Cao¹,

Gao²,

Xu³

et al. 2017

Anim Reprod

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Air phase is an indispensable environmental factor affecting oocyte maturation and early embryo development. Human exhaled air was previously proved to be a reliable and inexpensive atmosphere that sustains normal early development of mouse and bovine embryos. However, whether human exhaled air can support in vitro maturation (IVM) of porcine oocytes is not yet known. To evaluate the feasibility of maturing oocytes in human exhaled air, we examined oocyte morphology, BMP15 expression, nuclear and cytoplasmic maturation. We found that cumulus expansion status, expression levels of BMP15 important for cumulus expansion and the rate of first polar body emission were similar among human exhaled air, 5% O 2 or 20% O 2 in air after IVM of 44 h. Furthermore, the percentage of metaphase II (MII) oocytes showing normal cortical and sub-membranous localization of cortical granules and diffused mitochondrial distribution patterns is comparable among groups. The cleavage, blastocyst rate and total cell number were not apparently different for parthenogenetic activated and somatic cloned embryos derived from MII oocytes matured in three air phases, suggesting oocytes matured in human exhaled air obtain normal developmental competence. Taken together, human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development

Cao¹,

Gao²,

Xu³

et al. 2017

Anim Reprod

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“…Several studies on oocytes maturing in vitro reached the conclusion that the use of a reduced oxygen concentration leads to superior results [19][20][21]. However, other studies reported little or no benefit in using reduced oxygen concentrations during oocyte maturation [22,23]. Furthermore, some types of cells proliferated better in the 20% oxygen environment than in a reduced oxygen environment [24].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Hirao

Shimizu

Iga

et al. 2012

Journal of Reproduction and Development

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Abstract:The oxygen environment in cell culture has a significant impact on the health and performance of cells. Here, we compared the effects of reduced (5%) and ambient (20%) oxygen concentrations on bovine oocyte-granulosa cell complexes, each containing a growing oocyte 90-102 µm in diameter, cultured for 14 days. Both oxygen concentrations showed some advantages and disadvantages; in 5% oxygen, the survival rate of oocytes was significantly higher than in 20% oxygen, but the resulting oocytes were significantly smaller, which was a serious disadvantage. During the first 4 days of culture, the growth and viability of oocytes were satisfactory using 5% oxygen. This observation led us to examine the effect of changing the oxygen concentration from 5% to 20% on Day 4 in order to minimize the expected disadvantages of constant 5% and 20% oxygen. The largest population of fully grown oocytes was obtained from cultures in which the oxygen concentration was changed in this way, which also led to higher oocyte viability than in constant 20% oxygen. A similar tendency was found in the frequency of oocytes becoming blastocysts after in vitro fertilization. Surviving oocytes eventually became located within an enlarged dome-like structure, and although the 5% oxygen environment may have been appropriate for oocyte growth in the early stages, 20% oxygen may have been necessary for the growth of oocytes in the dome-like structure. These results indicate an effective way of modulating oxygen concentration according to the growth of oocyte-granulosa cell complexes in vitro. Key words: Bovine, Granulosa cell, Growing oocyte, In vitro, Oxygen concentration (J. Reprod. Dev. 58: [204][205][206][207][208][209][210][211] 2012) M ost of the growing oocytes stored in the mammalian ovary are eventually lost, and in order to be able to utilize them, it is important to develop culture systems that effectively support oocyte growth and the acquisition of developmental competence. We have previously described a 2-week culture system [1] in which bovine oocytes obtained from early antral follicles are able to complete the growth phase. However, the resulting oocytes are on average smaller than those grown in follicles in vivo. Further improvement of the culture system is therefore necessary.Our previous work comparing bovine oocyte growth in reduced (5%) and ambient (20%) oxygen concentrations [2] suggests the importance of oxygen optimization in determining culture conditions. The oxygen environment has significant effects on cell activities in culture in various cell types, such as somatic cells [3,4], embryos [5,6] and stem cells [7]. Although a universal optimal oxygen concentration for cell culture has not been determined, many studies on embryos [8][9][10], preantral follicles [11], testicular germ cells [12] and other cells [13][14][15][16][17] have suggested that a reduced oxygen concentration gives better results than an ambient oxygen concentration. A partial explanation for this would be that there is less cell damage asso...

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“…In contrast, there are many studies that have examined the effect of O 2 concentration during in vitro maturation of oocytes across several species. Examples of such studies include hamster (Gwatkin & Haidri 1974), squirrel monkey (Yeoman et al 1999), cow (Hashimoto et al 2000, pig (Park et al 2005) and mouse (Haidri et al 1971, Eppig & Wigglesworth 1995, Hu et al 2001. Such studies have not yielded any consensus over the optimal O 2 concentration during maturation, as there are conflicting results, especially in regard to the benefits of a low O 2 atmosphere.…”

Section: Introductionmentioning

confidence: 99%

Mathematical modelling of oxygen concentration in bovine and murine cumulus–oocyte complexes

Clark¹,

Stokes²,

Lane³

et al. 2006

Reproduction

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Immature oocytes benefit from nutrient modification of the follicular environment by the surrounding cumulus mass. However, the oxygen concentration that the oocyte may be exposed to could be lower than the antral follicular concentration due to the metabolism of surrounding cumulus cells. Using metabolic data previously determined, we have developed a mathematical model of O 2 diffusion across the bovine and murine cumulus-oocyte complex. From this we have determined that across a physiological range of external pO 2 , less than 0.25% and 0.5% O 2 is removed by cumulus cells within the bovine and murine cumulus-oocyte complex respectively. Our model differs from others as it: incorporates a term that allows for nonlinear variation of the oxygen consumption rate with oxygen concentration; considers two regions (oocyte and cumulus) sharing a common boundary, both of which consume oxygen at different non linear rates. Cumulus cells therefore remove little O 2 , thus sparing this essential gas for the oocyte, which is dependent on ATP generation via oxidative phosphorylation.

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High oxygen tension during in vitro oocyte maturation improves in vitro development of porcine oocytes after fertilization

Cited by 45 publications

References 33 publications

Human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development

Human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Mathematical modelling of oxygen concentration in bovine and murine cumulus–oocyte complexes

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